3,845 research outputs found
W. F. Mitchell
No full textEarlier this year, an article was published in the News Bulletin
(February 2012) on the background of Captain Boyns Hedley
Hocking, a dentist who became one of the first casualties in the
bombing of Darwin in 1942. The author, W F Mitchell, has kindly
provided a summary of the 70th anniversary activities held in
Darwin in February 2012 to commemorate this significant event in
the Northern Territory?s historyDate:2012-09News Bulletin no. 413, p. 36 - 37
Joni Mitchell: New Critical Readings
No full textJoni Mitchell: New Critical Readings presents a wide range of critical approaches for thinking about the oeuvre of musician and artist Joni Mitchell. It showcases work by leading academics from the fields of popular music and culture on subjects as varied as Mitchell’s environmentalism, the politics of ageing in her work, and her often fraught relationship with feminism.</p
Joni Mitchell: New Critical Readings
No full textJoni Mitchell: New Critical Readings presents a wide range of critical approaches for thinking about the oeuvre of musician and artist Joni Mitchell. It showcases work by leading academics from the fields of popular music and culture on subjects as varied as Mitchell’s environmentalism, the politics of ageing in her work, and her often fraught relationship with feminism.</p
David Mitchell, World Vision
No full textDavid Mitchell lives in Connecticut just outside of NYC and talks about how World Vision is strategizing to help support countries around the world during the COVID19 pandemic, as well as giving us insight regarding what it\u27s like to be close to NYC, which has become the new epicenter for the coronavirus in the US
Foreword: Assisted Reproductive Technology and the Law
No full textThis foreword introduces Issue 2: Assisted Reproductive Technology and the Law of the 35th Volume of the William Mitchell Law Review. It begins by outlining the author\u27s personal experience with ART, and contrasts her reasoning for using ART with the traditional need for ART. Finally, it lists some of the many legal questions yet to be conclusively answered
An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans
No full text
Candida albicans
is the major fungal pathogen of humans and is the subject of intense biomedical and discovery research. Until recently, the pace of research in this field has been hampered by the lack of efficient methods for genome editing. We report the development of a highly efficient and flexible genome editing system for use with
C. albicans
. This system improves upon previously published
C. albicans
CRISPR systems and enables rapid, precise genome editing without the use of permanent markers. This new tool kit promises to expedite the pace of research on this important fungal pathogen.
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Regulation of Yeast-to-Hyphae Transition in <i>Yarrowia lipolytica</i>
No full textThe yeast Yarrowia lipolytica undergoes a morphological transition from yeast-to-hyphal growth in response to environmental conditions. A forward genetic screen was used to identify mutants that reliably remain in the yeast phase, which were then assessed by whole-genome sequencing. All the smooth mutants identified, so named because of their colony morphology, exhibit independent loss of DNA at a repetitive locus made up of interspersed ribosomal DNA and short 10- to 40-mer telomere-like repeats. The loss of repetitive DNA is associated with downregulation of genes with stress response elements (5'-CCCCT-3') and upregulation of genes with cell cycle box (5'-ACGCG-3') motifs in their promoter region. The stress response element is bound by the transcription factor Msn2p in Saccharomyces cerevisiae. We confirmed that the Y. lipolytica msn2 (Ylmsn2) ortholog is required for hyphal growth and found that overexpression of Ylmsn2 enables hyphal growth in smooth strains. The cell cycle box is bound by the Mbp1p/Swi6p complex in S. cerevisiae to regulate G1-to-S phase progression. We found that overexpression of either the Ylmbp1 or Ylswi6 homologs decreased hyphal growth and that deletion of either Ylmbp1 or Ylswi6 promotes hyphal growth in smooth strains. A second forward genetic screen for reversion to hyphal growth was performed with the smooth-33 mutant to identify additional genetic factors regulating hyphal growth in Y. lipolytica. Thirteen of the mutants sequenced from this screen had coding mutations in five kinases, including the histidine kinases Ylchk1 and Ylnik1 and kinases of the high-osmolarity glycerol response (HOG) mitogen-activated protein (MAP) kinase cascade Ylssk2, Ylpbs2, and Ylhog1. Together, these results demonstrate that Y. lipolytica transitions to hyphal growth in response to stress through multiple signaling pathways. IMPORTANCE: Many yeasts undergo a morphological transition from yeast-to-hyphal growth in response to environmental conditions. We used forward and reverse genetic techniques to identify genes regulating this transition in Yarrowia lipolytica. We confirmed that the transcription factor Ylmsn2 is required for the transition to hyphal growth and found that signaling by the histidine kinases Ylchk1 and Ylnik1 as well as the MAP kinases of the HOG pathway (Ylssk2, Ylpbs2, and Ylhog1) regulates the transition to hyphal growth. These results suggest that Y. lipolytica transitions to hyphal growth in response to stress through multiple kinase pathways. Intriguingly, we found that a repetitive portion of the genome containing telomere-like and rDNA repeats may be involved in the transition to hyphal growth, suggesting a link between this region and the general stress response
Mitchell, Dr. Henry H. Interview of Willie Ella Asberry With E.P. Mitchell and Jack Catherill of Sacramento Side 1, Rev. Woody at 216 N. Bonny Brae Side two
No full textDr. Henry Mitchell interviews several people associated with the Pentecostal movement and the Azusa street revival.The Atlanta University Center Robert W. Woodruff Library acknowledges the generous support of the National Endowment for Humanities - Humanities Collections and Reference Resources Implementation Project Grant in supporting the processing and digitization of a number of its major archival collections as part of the project: Spreading the Word: Expanding Access to African American Religious Archival Collections at the Atlanta University Center Robert W. Woodruff Library.</em
Publicpension governance and performance : lessons for developing countries
No full textThe author examines the relationship between public sector pension plan performance and management practices to improve the design and governance of public pensions in developing countries. Understanding this relationship is important because better yields on public pension plan investment reduce the need for additional taxes to support retirees - and well-funded plans stand a better chance of paying promised benefits. The author's model relates investment returns on public pension assets, as well as plan funding status, to features characterizing the pension systems'governance structure and authority, using new data set on U.S. state and local public sector plans. The following findings stand out. The higher the fraction of retirees elected to the pension board, the stronger the negative effect on investment return in 1990, and the more variable the returns. Systems fared about the same whether they had in-house or external money managers, or independent performance analysis (even if the external managers were drawn from the top 10). But public pensions performed better when fund and actuarial computations were done by professional actuarial and investment counselors rather than relying on former or current employees to choose investment strategies. Social investment rules hurt public pension yields. Public pension plans which mandated that a certain portion of investments be director to instate projects generated much lower returns. The data show that many public pension systems funded their plans satisfactorily but others did not. The results show the following. Fiscal stress reduced stock funding ratios. Stock funding rates were lower, the higher the fraction of elected retirees and elected active workers represented on the pension system board. Stock funding ratios were higher when a system had in-house actuaries, when the board authorized benefit levels, and when board members had liability insurance. Stock funding rates were unaltered by state statutes guaranteering that benefits be guaranteed by law, or by legally set funding requirements, or by the state's ability to carry budget deficits from one year to the next. Nor did they vary when dedicated or special taxes were earmarked for pension revenue. Policymakers in developing countries can profit from the mistakes made and lessons learned by U.S. pension analysis. Although no single package of pension plan practices can optimize investment performance for all systems across all time periods, care must be taken when designing the regulatory and investment environment in which these plans operate. Developing countries should study the work of the U.S. Government Accounting Standards Board. The author discusses some of the complex issues that must be confronted when establishing funding norms for defined benefit pension plans in the public sector.ICT Policy and Strategies,Financial Intermediation,Economic Theory&Research,Pensions&Retirement Systems,Economic Stabilization
Common features of fungal biofilms.
No full text<p>Gene expression has been compared between planktonic cells and biofilm cells of both <i>A. fumigatus</i> and <i>C. albicans</i>. The major functional categories of genes upregulated in biofilms are summarized in the blue box. The micrographs below show a cryo SEM view of an <i>A. fumigatus</i> biofilm (left; Stephanie Guadagnini, Anne Beauvais, and J.P. Latge, Institut Pasteur, Paris, France) and a scanning EM view of <i>C. albicans</i> in vitro biofilm cells (right; Fanning, Suhan, and Mitchell, unpublished). In both cases, extracellular matrix (ECM) is evident.</p
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