1,720,998 research outputs found
An evaluation study of enzyme-linked immunosorbent assay (ELISA) using recombinant GRA1 protein for detection of igg antibodies againts Toxoplasma gondii infections
No full textReliable laboratory testing is important to detect Toxoplasma gondii infection and focuses on improving the low cost and easy to use the diagnostic instrument. Enzyme-linked immunosorbent assay (ELISA) method can be used to determine a large number of samples within a short period of time which based on antibody or antigen detection. We tested the sensitivity and specificity of GRA1 protein of as antigen using the ELISA method to toxoplasmosis diagnosis and compared it with commercial ELISA kit. Seventy sera samples were collected and tested using indirect ELISA, commercial ELISA kit and GRA1 protein-coated as antigen. The results showed 48 and 51 samples had positive IgG antibody using ELISA-GRA1 and commercial ELISA kit, respectively. The GRA1 sensitivity and specificity on ELISA were 100% and 86.36%, respectively. Whereas positive predictive value (PPV) was 94.11%. This result indicated that the recombinant GRA1 protein is a highly immunogenic protein in human toxoplasmosis and marker for toxoplasmosis screening
KARAKTERISASI ANTIBODI MONOKLONAL TERHADAP PROTEIN MEMBRAN Toxoplasma gondii ISOLAT LOKAL
No full textKeragarnan Genetik Gen NADH Dehydrogenase Subunit 6 pada Monyet Hantu (Tarsius Sp.)
No full textTarsius merupakan< pri~ata yang memiliki daya tarik tersendiri karena merriiliki ukuran tubuh
yang sangat kecil dan bola mata yang besar. Berdasar habitat, Tarsius adalah salah satu sa twa endemik
Indonesia yang jumlah populasinya mulai memprihatinkan. Usaha untuk melestarikan Tarsius sp., perlu
dilakukan suatu tindakan melalui upaya konservasi baik dilakukan secara in~situ maupun ex-situ. Dalam
upaya konservasi, identifikasi terhadap spesies Tarsi us berdasar pada karakter morfologi dan molekuler
sangatlah diperlukan, karena identifikasi berdasarkan morfologi dan voaklisasi seperti yang selama ini
dilakukan masih belum tepat, sehingga diperlukan peneguhan identifikasi. Tujuan dari penelitian ini
adalah untuk mengkaji adanya keragaman genetik dari gen ND6 Tarsius sp. dan diharapkan dapat
digunakan sebagai penanda genetik untuk mengidentifikasi Tarsius sp. dan mengungkap afiliasi, hubungan
filogen:·~tik Tarsius sp. dengan anggota primata lain. Sampel diperoleh dari beberapa habitat asal Tarsius
sp. yaitu Sulawesi Utara sebanyak tiga ekor, Sulawesi Tengah satu ekor, Kalimantan tiga ekor dan dari
Sumatera Selatan sebanyak tiga ekor. Hasil isolasi DNA selanjutnya digunakan sebagai cetak.an untuk
ampli.fikasi fragmen DNA dengan teknik Polymerase chain reaction (PCR). Amplikon (produk PCR) diperoleh
566 bp dan 629 bp. Hasil sekuensing nukleotida didapatkan 513 nukleotida, terdapat 126 nukle~tida dan
46 asam amino yang berbeda. Jarak genetik paling kecil 0%, paling tinggi 30,2% dan rataan 16,3%.
Sekuen nukleotida dan asam amino ND6 dapat digunakan sebagai penanda genetik an tara T. spectrum,
T. dianae, dan T. bancanus namun tidak dapat sebagai penanda genetik antara T. bancanus asal Sumatra
dan asal Kalimantan.
KARAKTERISASI ANTIBODI MONOKLONAL TERHADAP PROTEIN MEMBRAN Toxoplasma gondii ISOLAT LOKAL
No full textIsolasi dan identifikasi ekspresi protein rekombinan granule-1 (GRA-1) Toxoplasma gondii isolat lokal
No full textToxoplasmosis is a diseases caused by intracellular protozoa called Toxoplasma gondii
Deteksi Genotipe K-Kasein dengan Teknik Polymerase Chain Reaction-Single Strand Conformational Polymorphism dan Efek Genetiknya terhadap Kadar Protein dan Lemak Susu Kambing Peranakan Etawah
Full text linkArtikel dalam bentuk PD
Aktivitas Fagositosis Makrofag Peritoneum dan Sel-Sel Penghasil IgA dalam Jaringan Intestinum pada Mencit Yang Diberi Laktoferin Susu Kuda
No full textLactoferin is an iron-binding glycoprotein, has a very broad range of biological functions relating to the host defence system, e.g as immunomodulator. The aim of this research were to study of horse milk lactoferrin glycan type, and immune responses (phagocytosis activity and IgA producing cells) of mice fed horse milk lactoferin. Horse milk lactoferin was purified on cation exchange chromatography and gel filtration, and glycan type was analyzed with spesific lectin. Female Balb/c mice 6-8 weeks old, fed 0.0 (control); 0.1; 0.5; 1.0 mg/mice/day of horse milk lactoferin orally, in assay period of 28 day. In vitro phagocytosis activity were assayed with latex beads, and IgA producing cells was analyzed with IHC (immunohistochemical) methods. The result showed that molecular weight of horse milk lactoferin was 79.18 kDa, and its glycan type reacted with WGA (wheat germ agglutinine), but not with Con-A (concanavalin A) lectin. The phagocyte cells and IgA producing cells of mice fed horse milk lactoferin were higher than the control mice. In conclusion, horse milk lactoferin had complex type glycan and could stimulate the mice immune responses through the increasing phagocytosis activity of periteal macrophage and IgA producing cells in the intestinal tissue
Key Words: Horse Milk Lactoferin, Glycan, Phagocytosis, IgA Producing Cell
Study of Local Herb Potency as Rumen Modifier: Red Ginger (Zingiber Officinale Var. Rubrum) Addition Effect on In Vitro Ruminal Nutrient Digestibility
Full text linkRed ginger is herb that commonly used as part of traditional medicine due to its essential oil content. Some essential oil have proven as rumen fermentation modifier. Addition of red ginger in ruminant diet was studied using in vitro gas production technique to evaluate its effect on nutrient digestibility. Red ginger meal was added to meet essential oil level in fermentation medium of 0 mg/l as control, 25, 50, 75 and 100 mg/l. The diet consisted of Penisetum hybride, rice bran, wheat pollard in ratio 60:20:20 DM basis. Feed fermentation was incubated for 24 h at 39°C. At the end of incubation data of gas production volume was taken, and residual feed were collected for further nutrient analysis to calculate the digestibility of dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF). Variance analysis was used to analysis collected data, followed by DMRT analysis. Addition of red ginger increase total volume of gas production at level 50 mg/L and above. DM and OM digestibility did not affected by red ginger addition whereas CP digestibility were significantly decreased start at level 50 mg/l. In contrast, CF digestibility of treatment level of 50 mg/l significantly higher than control. In conclusion, addition of red ginger correspond to level essential oil of 50 to 100 mg/l improve ruminal nutrient fermentation
Kajian Molekuler Daerah D-Loop Parsial Deoxyribonucleic acid (DNA) Mitokondria Kuda (Equus caballus) Asli Priangan
No full textThe Effect of Cumin (Cuminum cyminum) Addition as Source of Essential Oils on Nutrien Digestibility, VFA, Amonia and Methan Production
No full textIn vitro gas production technique was used to evaluate the effects of different doses of cumin (Cuminum cyminum) on rumen in vitro nutrient digestibility, VFA, ammonia and methane production. Addition of cumin correspond to essential oil levels of 0, 25, 50, 75 and 100 mg/L of fermentation medium, Fermentation was conducted according to Menke and Steingab methode for 24h. After 24 h, incubation was stopped and gas produced in each fermenter were collected to measured methane production, then fermentation broth was filtered to determine nutrient digestibility from the residual feed. Filtrate was used as sample for VFA and ammonia determination. Addition of cumin did not has detrimental effect on rumen fermentation. It did not alter pH, dry matter and organic matter digestibility, VFA and methane production. However ammonia concentration, crude protein and crude fiber digestibility were changed by cumin addition. Crude protein digestibility at cumin level 75 and 100 mg/L lower than control and cumin levels 25, 50 mg/L groups. Ammonia concentration reduced as effect of cumin addition. Crude fiber digestibility was higher at all level of cumin addition. In conclusion, cumin have capability to alter rumen fermentation and it has been potency to be used as additive
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