1,720,984 research outputs found
A fucose-containing O-glycoepitope on bovine and human nucleolin
No full textIn this paper, we demonstrate the existence and localization
of fucosyl-containing O-glycoforms of nucleolin in cultured
bovine endothelial cells (CVEC) and malignant cultured
human A431 cells. The tool for this discovery was an
antibody raised against gp273, a glycoprotein ligand for the
sperm–egg interaction in the mollusc bivalve Unio elongatulus.
The function and immunological properties of gp273
mainly depend on clustered Lewis-like, fucose-containing
O-glycans. Here an anti-gp273 antibody was used to evaluate
whether glycoepitopes similar to those of gp273 are part
of potential ligands of selectins in endothelial cells.We found
that anti-gp273 strongly and exclusively interacted with a
110 kDa protein in CVEC and A431 tumor cells. After partial
purification, mass spectrometry identified the protein
as nucleolin. This was confirmed by comparing anti-gp273
and anti-nucleolin antibody immunoblotting after nucleolin
depletion.We confirmed that anti-gp273 binding to nuclear
and extranuclear nucleolin was against a fucose-containing
O-glycoepitope by immunoblot analysis of the protein after
chemically removing O-glycans and by lectin-blot analysis
of control and nucleolin-depleted samples. Using anti-gp273
IgG, we detected nucleolin on the plasma membrane and
cytoplasm. O-glycosylation may regulate the plethora of
functions in which nucleolin is involved
Phosphoprotein modulated by Sel1L induced expression in MCF-7 human mammari carcinoma cell line
No full textFUNCTIONAL PROTEOMIC INVESTIGATION OF AN ESCHERICHIA COLI K-12 MUTANT EXPRESSING A GASP PHENOTYPE
No full textImmunoproteomics of myasthenia
No full textAutoantibodies to nAChR are found in 85% of patients with Myasthenia Gravis (seropositive) while in 15% of them the standard immunoprecipitation test for anti-AChR is negative (seronegative). Some of these seronegative patients have antibodies against muscle specific Kinase ( MuSK). The aim of this study is to try to define auto-antibodies against protein different from AChR and MuSK in seronegative patients. We used the cell line TE671, that expresses human AChR. Through our proteomic approach we identified some proteins that could represent potential antigens different from AChR and MuSK
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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