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New emergent methodologies for an easy detection of analytes of high clinical relevance. The case of celiac disease
No full textThe autochthonous agerolese breed: genetic structure at CSN1S1 and CSN3 loci.
No full textThis work arises as targets the genetic characterization at CSN1S1 and CSN3 loci, using techniques of
molecular biology, of the agerolese cattle, an autochthonous genetic type reared in Campania, which is at
risk of extinction.In particular, we proceeded to the identification of the CSN1S1 G and CSN3 B allele
carriers, whose choice was dictated by the documented importance of their products (as1???CnG and k???
CnB).The molecular event that characterizes the CSN1S1 G allele is an insertion of a truncated retroposonlike
element (LINE) of 371 bp realized between nucleotides 58 and 59 of 19th exon. Consequently, the 19th
exon of bovine CSN1S1 G allele appears to be 756 bp long instead of 385 bp[1].The cause of a reduced
efficiency of protein synthesis has been hypothesized to be an interaction between the LINE nucleotide
sequence and the poly(A) sequence of the mature transcript, with consequent reduction of mRNA stability
and its rapid degradation.The mutation responsible for the CSN3 B allele is a nucleotide substitution
(GATAsp???GCTAla) at nucleotide 416 of 4th exon. This mutation is located relatively close to several
glycosylation sites and probably affects the structure of the protein and glycosylation patterns[2]. It was
reported that the CSN1S1 G allele is responsible for a considerable reduction of the content of as1 casein
that involves a lower coagulation time and curd firmness of milk compared to that produced by cows
bearers of the remaining alleles[3].Similarly, it was shown that the k???CnB variant influences the processes
of cheesemaking, giving the milk a better attitude to cheese, with lower clotting times and firming, greater
consistency of the curd and higher yield. From the milk obtained by the CSN3 BB cows it can get a higher
yield in "Parmigiano Reggiano" equal to ~10%[4]
The genomic DNA was extracted from 79 individual samples of bovine blood reared in different farms
located in Campania. For the genotyping of CSN1S1 G allele carriers was applied a PCR protocol[1].The
genotyping of CSN3 B allele was achieved by means of Hinf I PCR???RFLP[5]
Indicating with N the other alleles at CSN1S1 and CSN3 loci, the genotype distribution of investigated
population was 74N/N, 5N/G, 0G/G for CSN1S1 with a G allele frequency of 0.03 and 25N/N, 33N/B, 21B/B
for CSN3 with a B allele frequency of 0.47
It can be assumed that the evidenced genetic variability at CSN3 locus for the agerolese cattle genetic type
plays an important role in the determination of some fundamental parameters, both from the nutritional
point of view that technological, for the production and quality of milk and of DOP products derived from it
as the historians cheeses "Provolone del Monaco" and "Fior di latte??
Genetic variability of Prolactin (PRL) and Prolactin Receptor (PRLR) genes in the Italian Mediterranean river buffalo.
No full textIn mammals, the hormone prolactin (PRL) is best known for its role in the regulation of lactation (Lee et al. 2007). PRL affects multiple reproductive and metabolic functions through its receptors (PRLR), characterized by the ability to activate Janus kinase 2 and signal transducer and activator of transcription (Fleenor et al. 2006). PRL is an anterior pituitary peptide hormone involved in many endocrine activities essential for reproductive success (Vaclavicek et al. 2006).
Several polymorphic sites have been detected within PRL and PRLR genes, and significant associations among their variants with milk production traits have been described in dairy cattle (Alfonso et al. 2012; Zhang et al. 2008).
PRL (EMBL EF054878) and PRLR (EMBL GQ339914) genes consist of 5 and 9 exons, coding for a protein of 219 and 581 amino acids, respectively.
A partial sequencing of PRL exon 5 and PRLR exon 9 performed on 10 Italian Mediterranean river buffaloes allowed us to identify 1 SNP for PRL (C108→T, numbering from the 1st nt of exon 5) and 7 SNPs for PRLR (G128→A, C374→T, G517→A, C520→A, C622→T, G626→A and T707→C, numbering from the 1st nt of exon 9). The PRL SNP is silent (Leu202) as well as 626 (Ala494) and 707 (Asp521) PRLR SNPs. The other 5 PRLR SNPs are responsible of the following amino acid changes: His328→Arg, Ala410→Val, Asp458→Asn, Gln459→Lys and Thr493→Met.
574 buffaloes of several farms in Salerno province (South Italy) were genotyped in out-sourcing (http://kbioscience.co.uk) for 108 PRL and 128 PRLR SNPs. The genotype distribution of investigated population was 10 C/C, 122 C/T and 442 T/T (frequency of T allele 0.87) for PRL and 9 G/G, 108 G/A and 457 A/A for PRLR (frequency of A allele 0.89). For both SNPs, the investigated population was in Hardy–Weinberg equilibrium. Such polymorphisms could represent useful genetic markers for association studies with quali-quantitative milk characteristics, but further studies are needed to evaluate their potential use
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
New trends in bio/nanotechnology: stable proteins as advanced molecular tools for health and environment
No full textIn this work the thermophilic trehalose/maltose-binding protein from Thermococcus litoralis is presented as a probe
for the design of a high stable fluorescence biosensor for glucose. In particular, we show the possibility of modulating
the protein specificity by changing temperature. In addition to glucose sensing, we also report on the possibility of
utilizing odorant-binding proteins as a probe for the development of optical sensors for analytes of environmental
interests
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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