1,725,607 research outputs found
The heat-shock protein Apg-2 binds to the tight junction protein ZO-1 and regulates transcriptional activity of ZONAB
No full textThe tight junction adaptor protein ZO-1 regulates intracellular signaling and cell proliferation. Its Src homology 3 (SH3) domain is required for the regulation of proliferation and binds to the Y-box transcription factor ZO-1-associated nucleic acid binding protein (ZONAB). Binding of ZO-1 to ZONAB results in cytoplasmic sequestration and hence inhibition of ZONAB's transcriptional activity. Here, we identify a new binding partner of the SH3 domain that modulates ZO-1-ZONAB signaling. Expression screening of a cDNA library with a fusion protein containing the SH3 domain yielded a cDNA coding for Apg-2, a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 heat-shock proteins, which is overexpressed in carcinomas. Regulated depletion of Apg-2 in Madin-Darby canine kidney cells inhibits G(1)/S phase progression. Apg-2 coimmunoprecipitates with ZO-1 and partially localizes to intercellular junctions. junctional recruitment and coirnmunoprecipitation with ZO-1 are stimulated by heat shock. Apg-2 competes with ZONAB for binding to the SH3 domain in vitro and regulates ZONAB's transcriptional activity in reporter gene assays. Our data hence support a model in which Apg-2 regulates ZONAB function by competing for binding to the SH3 domain of ZO-1 and suggest that Apg-2 functions as a regulator of ZO-1-ZONAB signaling in epithelial cells in response to cellular stress
Regulation of tight junction assembly and epithelial morphogenesis by the heat shock protein Apg-2
No full textBackground: Tight junctions are required for epithelial barrier formation and participate in the regulation of signalling mechanisms that control proliferation and differentiation. ZO-1 is a tight junction-associated adaptor protein that regulates gene expression, junction assembly and epithelial morphogenesis. We have previously demonstrated that the heat shock protein Apg-2 binds ZO-1 and thereby regulates its role in cell proliferation. Here, we addressed the question whether Apg-2 is also important for junction formation and epithelial morphogenesis.Results: We demonstrate that depletion of Apg-2 by RNAi in MDCK cells did not prevent formation of functional tight junctions. Similar to ZO-1, however, reduced expression of Apg-2 retarded de novo junction assembly if analysed in a Ca-switch model. Formation of functional junctions, as monitored by measuring transepithelial electrical resistance, and recruitment of tight and adherens junction markers were retarded. If cultured in three dimensional extracellular matrix gels, Apg-2 depleted cells, as previously shown for ZO-1 depleted cells, did not form hollow polarised cysts but poorly organised, irregular structures.Conclusion: Our data indicate that Apg-2 regulates junction assembly and is required for normal epithelial morphogenesis in a three-dimensional culture system, suggesting that Apg-2 is an important regulator of epithelial differentiation. As the observed phenotypes are similar to those previously described for ZO-1 depleted cells and depletion of Apg-2 retards junctional recruitment of ZO-1, regulation of ZO-1 is likely to be an important functional role for Apg-2 during epithelial differentiation
APG III: Bibliographical Information and Synonymy of Magnoliidae Abstract Kew words Introduction
No full textReveal, James L., Chase, Mark W., Iii, -Apg, Iii, Apg (2011): APG III: Bibliographical Information and Synonymy of Magnoliidae Abstract Kew words Introduction. Phytotaxa 19 (1): 71-134, DOI: 10.11646/phytotaxa.19.1.4, URL: http://biotaxa.org/Phytotaxa/article/view/phytotaxa.19.1.
„Person-/Klientenzentrierte Supervision und Organisationsentwicklung“: Statut und Ausbildungsordnung von ÖGwG und IPS der APG
No full textPERSON dokumentiert im folgenden das Statut mitsamt der Ausbildungsordnung der neuen gemeinsamen Aus-, Fort-und Weiterbildung „Person-/Klientenzentrierte Supervision und Organisationsentwicklung“ des Instituts für Personzentrierte Studien der APG (IPS) und der ÖGwG. Im Anhang finden sich Hinweise für die Anrechenbarkeit für Personen, die bereits eine Ausbildung bei APG oder ÖGwG absolviert haben oder dort in Ausbildung sind
A new scaling for adverse pressure gradient turbulent boundary layers
No full textA new scaling for strong adverse pressure gradient (APG) turbulent boundary layers (TBL) is presented. The new scaling is applied to both the author’s unsteady and steady APG TBL experiments as well as several previously published studies. The scaling is shown to provide a remarkable collapse of the mean velocity profiles in each case. The new scaling is motivated by the recognition that the physics of the strong APG TBL is dominated by the inflectional instability of an embedded shear layer within the boundary layer. The implications of the scaling on the physics of APG TBL flows in general is also discussed
Cyrillaceae Lindl., Veg. Kingd.
No full text343. Cyrillaceae Lindl., Veg. Kingd.: 445. 14–28 Mar 1846, nom. cons.Published as part of Reveal, James L., Chase, Mark W., Iii, - Apg & Iii, Apg, 2011, APG III: Bibliographical Information and Synonymy of Magnoliidae Abstract Kew words Introduction, pp. 71-134 in Phytotaxa 19 (1) on page 104, DOI: 10.11646/phytotaxa.19.1.
Platanaceae T. Lestib., Botanogr. Elem.
No full text117. Platanaceae T.Lestib., Botanogr. Elém.: 526. 12–19 Jun 1826, nom. cons.Published as part of Reveal, James L., Chase, Mark W., Iii, - Apg & Iii, Apg, 2011, APG III: Bibliographical Information and Synonymy of Magnoliidae Abstract Kew words Introduction, pp. 71-134 in Phytotaxa 19 (1) on page 84, DOI: 10.11646/phytotaxa.19.1.
Byblidaceae Domin, Acta Bot. Bohem.
No full text384. Byblidaceae Domin, Acta Bot. Bohem. 1: 3. 1922, nom. cons.Published as part of Reveal, James L., Chase, Mark W., Iii, - Apg & Iii, Apg, 2011, APG III: Bibliographical Information and Synonymy of Magnoliidae Abstract Kew words Introduction, pp. 71-134 in Phytotaxa 19 (1) on page 110, DOI: 10.11646/phytotaxa.19.1.
Acorales Mart., Consp. Regn. Veg.
No full text9. Acorales Mart., Consp. Regn. Veg.: 6. Sep–Oct 1835Published as part of Reveal, James L., Chase, Mark W., Iii, - Apg & Iii, Apg, 2011, APG III: Bibliographical Information and Synonymy of Magnoliidae Abstract Kew words Introduction, pp. 71-134 in Phytotaxa 19 (1) on page 75, DOI: 10.11646/phytotaxa.19.1.
Picramniaceae Fernando & Quinn
No full text234. Picramniaceae Fernando & Quinn, Taxon 44: 177. 15 Mai 1995Published as part of Reveal, James L., Chase, Mark W., Iii, - Apg & Iii, Apg, 2011, APG III: Bibliographical Information and Synonymy of Magnoliidae Abstract Kew words Introduction, pp. 71-134 in Phytotaxa 19 (1) on page 94, DOI: 10.11646/phytotaxa.19.1.
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