1,725,332 research outputs found
Carnets de littératures africaines
No full text Le carnet diffuse des informations, des appels, des résultats, des chroniques, des analyses... en relation avec la recherche en littératures africaines. Il est animé par des membres de l'Association pour l'Etude des Littératures Africaines (APELA), association sans but lucratif ("loi 1901"), fondée en septembre 1983 par un groupe de chercheurs qui se sont rassemblés pour promouvoir les domaines littéraires africains et les travaux critiques qui les concernent : toutes les productions littéra..
Arts et textes en Afrique
No full textVous les cherchiez depuis longtemps ! ils ont été retrouvés grâce à Michel Naumann et sont désormais accessibles en ligne : les actes du colloque Arts et textes en Afrique. Cingal, Guillaume ; Naumann, Michel (éd.). Arts et textes en Afrique [Actes de la journée d'études du 22/09/2000]. Paris : Daniel Delas ; APELA, 2000, 97 p. Le volume intégral [pdf] peut être téléchargé sur Mukanda : https://mukanda.univ-lorraine.fr/s/mukanda/item/83592 Sommaire détaillé et liens de téléchargement des arti..
Povezanost oglašivačkih apela i namjere kupnje
No full textOvaj diplomski rad bavi se oglašivačkim apelima i povezanošću apela s namjerom kupnje. Ideja rada je pomoći oglašivačima u odabiru pravog oglašivačkog apela i omogućiti im da bolje prepoznaju koji su apeli prikladni za pojedine ciljne skupine. U teorijskom dijelu rada definiran je pojam oglašavanja, oglašivačkih apela, njihova podjela i kupovne odluke potrošača. U praktičnom dijelu ispitane su reakcije ispitanika na oglase s različitim apelima prema spolu i generacijama. Analizom rezultata ankete došlo se do zaključaka da postoje razlike među spolovima kao i različitim dobnim skupinama. Kod apela humora i seksa muškarci su pokazali veće sviđanje i veću sklonost kupnji oglašavanog proizvoda. Mlađe generacije Y i Z su te kojima se humoristični oglasi više sviđaju, a generacija Z sklonija je kupnji u odnosu na najstariju generaciju X. Oglasi s apelom seksa više se sviđaju najstarijoj generaciji X nego generaciji Z, a među preostalim istraživanim apelima (rješenje problema, komparativno oglašavanje i apel topline) ne postoje razlike niti prema spolu niti prema generacijama
Povezanost oglašivačkih apela i namjere kupnje
No full textOvaj diplomski rad bavi se oglašivačkim apelima i povezanošću apela s namjerom kupnje. Ideja rada je pomoći oglašivačima u odabiru pravog oglašivačkog apela i omogućiti im da bolje prepoznaju koji su apeli prikladni za pojedine ciljne skupine. U teorijskom dijelu rada definiran je pojam oglašavanja, oglašivačkih apela, njihova podjela i kupovne odluke potrošača. U praktičnom dijelu ispitane su reakcije ispitanika na oglase s različitim apelima prema spolu i generacijama. Analizom rezultata ankete došlo se do zaključaka da postoje razlike među spolovima kao i različitim dobnim skupinama. Kod apela humora i seksa muškarci su pokazali veće sviđanje i veću sklonost kupnji oglašavanog proizvoda. Mlađe generacije Y i Z su te kojima se humoristični oglasi više sviđaju, a generacija Z sklonija je kupnji u odnosu na najstariju generaciju X. Oglasi s apelom seksa više se sviđaju najstarijoj generaciji X nego generaciji Z, a među preostalim istraživanim apelima (rješenje problema, komparativno oglašavanje i apel topline) ne postoje razlike niti prema spolu niti prema generacijama
Apela promotes blood vessel regeneration and remodeling in zebrafish
No full textAbstract In contrast to adult mammals, zebrafish display a high capacity to heal injuries and repair damage to various organs. One of the earliest responses to injury in adult zebrafish is revascularization, followed by tissue morphogenesis. Tissue vascularization entails the formation of a blood vessel plexus that remodels into arteries and veins. The mechanisms that coordinate these processes during vessel regeneration are poorly understood. Hence, investigating and identifying the factors that promote revascularization and vessel remodeling have great therapeutic potential. Here, we revealed that fin vessel remodeling critically depends on Apela peptide. We found that Apela selectively accumulated in newly formed zebrafish fin tissue and vessels. The temporal expression of Apela, Apln, and their receptor Aplnr is different during the regenerative process. While morpholino-mediated knockdown of Apela (Mo-Apela) prevented vessel remodeling, exogenous Apela peptide mediated plexus repression and the development of arteries in regenerated fins. In contrast, Apela enhanced subintestinal venous plexus formation (SIVP). The use of sunitinib completely inhibited vascular plexus formation in zebrafish, which was not prevented by exogenous application. Furthermore, Apela regulates the expression of vessel remolding-related genes including VWF, IGFPB3, ESM1, VEGFR2, Apln, and Aplnr, thereby linking Apela to the vascular plexus factor network as generated by the STRING online database. Together, our findings reveal a new role for Apela in vessel regeneration and remodeling in fin zebrafish and provide a framework for further understanding the cellular and molecular mechanisms involved in vessel regeneration
Expression and function of APELA : a potential regulator of cell growth in human cancers
No full textApela, a novel gene identified by our laboratory, is expressed in mouse definitive endoderm, neural tube, and mouse embryonic stem cells (mESCs). In humans, APELA is expressed in embryonic stem cells, induced adult pluripotent stem cells (iPSCs) as well as adult kidney and prostate. APELA peptide signals through the G-protein coupled receptor, the APJ receptor, to regulate zebrafish definitive endoderm migration and cardiac development. Interestingly, the mRNA of Apela can mediate p53-dependent mESCs cell apoptosis. These findings suggest that Apela can functions as a peptide or as a lncRNA. Signaling pathways that are critical during embryogenesis are also important in cancer development and progression. However, thus far, whether APELA exerts any biological functions that regulate cancer progression is completely unknown. In this study, analysis of the cancer genome atlas (TCGA) RNA sequencing datasets reveals that APELA mRNA is expressed in different human cancer including in ovarian cancer. Real-time quantitative PCR analyses of clinical human ovarian cancer samples show that APELA mRNA levels are higher in ovarian clear cell carcinoma (OCCC), than other subtypes. Using a CRISPR/Cas9-mediated knockout approach, I have demonstrated that APELA knockout suppresses cell growth in the ovarian clear cell carcinoma cell line, OVISE. Decreased cell growth induced by APELA knockout can be partially attenuated by treating cells with recombinant human APELA protein. In addition, flow cytometry analyses show that APELA knockout induces G2/M phase arrest in OVISE cells. Western blot results show that the phosphorylation levels of ERK1/2, AKT, and cyclin B1 expression levels are significantly down-regulated in the APELA deficient OVISE cells. Moreover, our results indicate that in the APELA knockout cells, decreased cell growth is dependent on the expression of wildtype p53. Unexpectedly, knockout APELA does not affect cell growth in Ewing sarcoma cell line A673, which has high expression of APELA at mRNA level. Interestingly, the APJ receptor is expressed in A673 cells but not in OVISE cells, which strongly suggests that APELA can exert its function through APJ-independent pathway in OVISE cells. In summary, our study demonstrates that APLEA may be an important factor that mediates the progression of OCCC.Medicine, Faculty ofGraduat
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
APELA Expression in Glioma, and Its Association with Patient Survival and Tumor Grade
No full textGlioblastoma (GBM) is the most common and deadliest primary adult brain tumor. Invasion, resistance to therapy, and tumor recurrence in GBM can be attributed in part to brain tumor-initiating cells (BTICs). BTICs isolated from various patient-derived xenografts showed high expression of the poorly characterized Apelin early ligand A (APELA) gene. Although originally considered to be a non-coding gene, the APELA gene encodes a protein that binds to the Apelin receptor and promotes the growth of human embryonic stem cells and the formation of the embryonic vasculature. We found that both APELA mRNA and protein are expressed at high levels in a subset of brain tumor patients, and that APELA is also expressed in putative stem cell niche in GBM tumor tissue. Analysis of APELA and the Apelin receptor gene expression in brain tumor datasets showed that high APELA expression was associated with poor patient survival in both glioma and glioblastoma, and APELA expression correlated with glioma grade. In contrast, gene expression of the Apelin receptor or Apelin was not found to be associated with patient survival, or glioma grade. Consequently, APELA may play an important role in glioblastoma tumorigenesis and may be a future therapeutic target
An Apela RNA-Containing Negative Feedback Loop Regulates p53-Mediated Apoptosis in Embryonic Stem Cells
No full textSummaryMaintaining genomic integrity is of paramount importance to embryonic stem cells (ESCs), as mutations are readily propagated to daughter cells. ESCs display hypersensitivity to DNA damage-induced apoptosis (DIA) to prevent such propagation, although the molecular mechanisms underlying this apoptotic response are unclear. Here, we report that the regulatory RNA Apela positively regulates p53-mediated DIA. Apela is highly expressed in mouse ESCs and is repressed by p53 activation, and Apela depletion compromises p53-dependent DIA. Although Apela contains a coding region, this coding ability is dispensable for Apela’s role in p53-mediated DIA. Instead, Apela functions as a regulatory RNA and interacts with hnRNPL, which prevents the mitochondrial localization and activation of p53. Together, these results describe a tri-element negative feedback loop composed of p53, Apela, and hnRNPL that regulates p53-mediated DIA, and they further demonstrate that regulatory RNAs add a layer of complexity to the apoptotic response of ESCs after DNA damage
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