1,721,151 research outputs found
Modelli 3D della città e del territorio: uno strumento per l’analisi urbana e territoriale
Microtechnologies For Rapid Detection Of Aflatoxin M1 In Milk
In dairy industry, one of most pressing unmet needs is the timely detection of aflatoxin M1 contaminations that originate from animal feed and are secreted into milk and represents a hazard for human health and an economic loss for the dairy industry. The available technology for aflatoxin detection is laboratory-based, in most cases requires sample preparation and does not provide timely identification of contaminants, thus fails to deliver cost-effective management of milk quality. We have investigated further advancements with respect the traditional methodologies (e.g. ELISA, HPLC), by evaluating an novel twofold approach based on microfluidic technologies, biochemistry for sample preparation and photonic integrated sensors leading toward precision process management in dairy industry. Our aim was to develop a detection instrument2 that will integrate sample preparation and highly sensitive and selective label-free biosensing based on innovative photonic sensors functionalised with aptamers for the detection (Limit of detection < 50 ng/Kg) of aflatoxin M1 in milk samples. The main purpose of the sample preparation stage is to clean the milk sample from unwanted components like fats, which may interfere with the following stages and cause clogging of the system, and to concentrate and make the toxin available for detection. The core of the system is the detection module based on a low cost Si-based photonic device with aptamers to provide selectivity to targets and improved sensor stability. This work aim is to present the current technical achievements of this research activity and stimulate the discussion on bio-microtechnologies application in the dairy industry
Coronelli’s virtual globe
Nowadays globes are an important subject to study both for their history, their cartographic content and their artistic aspects. Moreover some softwares, such as Google Earth, Virtual Globe and many others, are very widespread because of their usability and, above all, because they represent a complex object, the Earth, in a intelligible way. This works deals with the possibility of applying digital technologies to the cartographic globes, especially Coronelli’s one, to obtain a virtual copy to navigate and to query
Interacting with virtual reconstructions in museums: The etruscanning project
Starting from our experience in this domain, we discuss some fundamental concepts about the potentialities of the virtual reconstructions of cultural sites inside museums, with a specific focus on the communication needs, the design, the combination of media, the interaction interfaces, and the embodiment. We conceive a virtual reconstruction as a digital ecosystem, whose main peculiarities are (1) 3D reconstruction, (2) inclusivity, and (3) interactivity. A virtual reconstruction, in a wide sense, should integrate different levels of visualization, both realistic and symbolic; 3D models; metadata; storytelling; behaviors; and tools of visualization and interaction, in order to "reconstruct" and communicate a cultural context, an ecosystem where all the information is integrated. Despite the great advancements of the last years in the digitization process, computer graphics techniques, and archiving strategies, a basic limit of most of virtual museums is that they do not fire up the attention and the involvement of the public: they lack stimulating activities for visitors, narratives metaphors, and emotional impact. The interaction interfaces are not always simple to understand and to control in a few minutes, and they can generate a sense of frustration that causes users to abandon the application after a short and superficial approach. No gap should exist between knowledge and communication. But how can we translate the complexity of the knowledge in appealing to users and into simple applications that fit with the public's need? This article focuses on some communication rules and criteria that are often considered of minor importance by the researchers working in the field of digital cultural heritage but that are really essential to cultural transmission, especially inside museums. We believe that a stronger collaboration between research institutions and museums and among different disciplines would be recommended. Given this premise, we present the Etruscanning EU project, developed in 2011- 2013, focused on the virtual reconstruction of two important Etruscan tombs of the Orientalizing period: the Regolini-Galassi tomb in Cerveteri and the tomb n.5 of Monte Michele in Veii. © 2014 ACM
Fabrication of Single Crystal Silicon Micro-/Nanowires and transferring them to Flexible Substrates
This work describes the method to fabricate micro-structured single-crystal Si wires and ribbons from silicon-on-insulator (SOI) wafers using “topdown” fabrication strategy. The ordered wires of Si have been successfully transferred to flexible PDMS substrates. The processing and combining stiff and brittle materials like Si with highly compliant substrates such as PDMS is a challenging task. The result presented here is an important milestone for fabricating electronic circuits over large and ultra flexible substrates
An unconventional approach to impedance microbiology: detection of culture media conductivity variations due to bacteriophage generated lyses of host bacteria
A novel and unconventional approach to impedance microbiology has been under investigation. In our approach, solution conductivity variations are generated from bacteriophage lyses of infected host cells and the consequent release of conductive endoplasmic material. To sensitively detect the lysis, low conductive growth media have been developed. A microchip has been fabricated to perform the analysis. The microchip is made of two bare gold electrodes and PDMS microchamber of 36 nL volume. Escherichia coli and selective phages T4 have been used as case study. Proof-of-principle experiments are here presented and discussed. The method was characterised in a wide range between 104 and 108 CFU/mL, where linear relation was found between conductivity variation and cell concentration in a log10 vs. log10 plot. The method is suited to integration with sample preparation based on phage-functionalised magnetic beads. It has a potential detection limit below 1 CFU/chamber and a total assay time of less than 1 hour
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