1,721,065 research outputs found
Developmental Biology of Teleost Lymphocytes
No full textThis book is presented as a series of short overviews that report on the current state of various relevant fields of immunobiology from an evolutionary perspective. The overviews are written by authors directly involved in the research, and most are members of the IADCI or have otherwise been involved in the related research for their respective overview. This publication offers scientists and teachers an easy and updated reference tool
Immunity in Dicentrarchus labrax culture: in vivo effects of arginate immunostimulation.
No full textParameters of cellular and humoral immunity wereanalysed in Dicentrarchus labrax to evaluate themodulatory activity of Ergosan®, an algal extractcontaining 1% alginic acid. Four cycles using normal fishfeed formulation supplemented with 0.5% Ergosan wereperformed at 60-days intervals (15-days treatment + 45-days suspension). Serum complement (C), lysozyme andtotal proteins and tissue HSP were measured 15, 30 and45 days after the first 15-days feeding cycle (short-term)and 45 days after each feeding cycle over a 35-weeksperiod (long-term). The percentages of B and Tlymphocytes in PBL and gut were measured over longtermtrial using mAbs against homologous Ig and T cells.Short-term alginic acid treatment raised serum C activity(p<0.05) at 15 days and serum C and lysozyme, gill andliver HSP (p<0.05) at 30 days. No significant differenceswith control group were found at 45 days. Over the longtermperiod, innate and specific immune parameters,survival, growth performances and conversion index didnot differ in treated and control fish.An experimental group received an oral Vibrioanguillarum vaccine (AVL, UK) according to the followingadministration protocol: 5-days vaccine and 0.5%Ergosan diet, 5-days normal diet, 5-days vaccine andErgosan diet (vaccination cycle, total dose: 0.2 ml/fish).Controls received the same dose of vaccine and were fedwith normal diet throughout the cycle. Total and specificserum Ig, C activity and percentages of lymphocytes inPBL and gut were measured 30 days after the start of thevaccination cycle.Oral vaccine raised significantly PBL Ig+ cells (p<0.05)and gut T-cells (p<0.01) in the Ergosan group. The titre ofspecific serum anti-Vibrio Ig was higher (p<0.05) inErgosan group compared with controls, while total Ig wereunmodified. These effects were accompanied by a rise ofPBL (T-cells +12%, B-cells +27%), intestine lymphocytes(T-cells +17%) and serum C (+19%) compared withvaccinated controls.The present studies in the sea bass indicate veryinteresting immunostimulatory properties of alginate.This work was granted by MIPAF Projects 5C68 and5C116.[...
Immunohistochemical, ultrastructural and biochemical evidence of adrenal chromaffin cell subtypes in sea bass Dicentrarchus labrax (L.).
No full textStimulation of gut immune system by early administration of probiotic strains in Dicentrarchus labrax and Sparus aurata
No full textImpact of marine contaminants of emerging concern on the cetacean transcriptome
No full textContaminants of emerging concern (CECs) are widely distributed in the environment, but their occurrence and potential toxicity are only now being evaluated. CECs are increasingly being detected in the waters and many act as endocrine disruptor compounds (EDCs), causing a variety of effects on health. The worldwide distributed perfluorooctanoic acid (PFOA) and bisphenol A (BPA) are CECs falling in the EDCs category.Skin samples from the bottlenose dolphin (Tursiops truncatus), a top predator that spends its entire life in the water and therefore subject to accumulation and magnification of contaminants, were collected to analyze the impact that environmentally relevant concentrations of CECs may have on global gene expression. We combined transcriptomic analysis and ex vivoassays using small skin slices cultured and treated for 24 h with PFOA or BPA or vehicle.RNA from dolphin biopsies was labeled and hybridized to a species-specific oligomicroarray. The skin transcriptome displayed changes related to contaminant exposure, potentially predictive about long-term effects on health, being the genes affected involved in immune modulation, response to stress, lipid homeostasis, and development. Within the genes differentially expressed in the transcriptome after CECs treatment, 4 were tested as potential gene markers of anthropogenic contaminants exposure on skin samples from wild cetaceans. RNA from 12 individuals, including the species Stenella coeruleoalba, T.truncatus, and Grampus griseuswere sampled in 3 areas (Adriatic, Ionian and Tyrrhenian seas). Three out of the 4 genes tested showed higher expression in the samples collected from the Adriatic sea.The transcriptomic signature of a dolphin skin could be relevant as classifier for a specific contaminant whilst giving information of the specific geographic location where the marine mammal spent its life, due to the different impact on gene expression exerted by different contamination levels
Rotavirus di gruppo A circolanti nel Nord Italia: caratterizzazione molecolare e implicazioni ai fini vaccinali.
No full textFine cytology of interrenal cells in sea bass Dicentrarchus labrax (L.): influence of different salinity concentrations.
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Studies on the sea bass Dicentrarchus labrax (L.) immune system for its control in aquaculture.
No full textMonoclonal antibodies anti-thymocytes and T-cells (DLT15) and anti-Ig and B-cells (DLIg3) were developed and used to perform a more comprehensive analysis of the immune system of the sea bass.
The MAbs were useful to define the morpho-physiology of the immune system of this teleost species, the only one for which such tools are available. These will be indispensible for the monitoring of humoral and cellular responses after antigen challenge and fish vaccination in aquaculture
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