6 research outputs found
Enzyme immobilization: an update
No full textCompared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immo-bilized enzyme systems allows an easy recovery of both enzymes and products, multiple re-use of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and greater variety of bioreactor designs. This paper is a review of the recent literatures on enzyme immobilization by various techniques, the need for immobilization and different applications in industry, covering the last two decades. The most recent papers, patents, and reviews on immobilization strategies and application are reviewed
Purification and characterization of a novel thermostable luciferase from Benthosema pterotum
No full textInvestigating the effects of inelastic soil–foundation interface response on the seismic demand of soil–structure systems
No full textThe effect of inelastic response of the soil–foundation interface is explored on the seismic demand of structures attached on top of shallow foundations. An ensemble of 20 strong ground motions recorded on National Earthquake Hazards Reduction Program site class D was employed for analyzing soil–structure systems with the Winkler foundation model and an equivalent single-degree-of-freedom system as the superstructure. Results show that there are key parameters that control the amount of difference between the elastic and inelastic modeling of the soil at the soil–foundation interface. Depending on the structural aspect ratio, the elastic modeling leads to an overestimated result for the total lateral displacement demands. Also, more than 50% reduction in the superstructure demands is desired when nonlinear soil modeling is considered and foundation sliding is allowed. The benefits from the “shrinking-dominated rocking motion” can be acquired with inelastic soil material that limits the transferred inertial force into the superstructure.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
Lipoid congenital adrenal hyperplasia with cholestatic jaundice
No full textBACKGROUND AND OBJECTIVE: Lipoid congenital adrenal hyperplasia (LCAH) is the most severe form of adrenal hyperplasia and mutations in the StAR gene are the most common cause of the disease. Adrenal insufficiency and cholestasis are reported in few patients. The aim of this study was to report the results of treatment of two sisters with lipoid CAH and cholestatic jaundice. CASE REPORTS: Here, we present two sisters at the age of 30 and 60 days with conjugated hyperbilirubinemia and elevated liver enzymes and adrenal insufficiency. They had a 46,XY karyotype with external female genitalia without uterus and ovaries. LCAH was detected through electrolyte abnormalities, increased ACTH, decreased levels of cortisol and sex hormones and was confirmed by determination of exome sequencing and Sanger sequencing. In these patients, a homozygous mutation (c.653C>T) in exon 6 of STAR gene was identified. The patients were treated with 10 mg of hydrocortisone IV every 8 hours for 3 days; oral hydrocortisone was then administered at a dose of 2.5 mg every 8 hours and 0.2 mg fludrocortisone daily. One month after the therapy, levels of bilirubin and liver enzymes of these patients became normal. The first patient died 7 months after her mother stopped giving the drugs to the child. The other patient is now 9 years old. She is in good clinical condition as her treatment goes on. CONCLUSION: Considering the reported cases, adrenal lipoid hyperplasia should be considered as a rare cause of cholestasis with adrenal insufficiency in patients. © 2021, Babol University of Medical Sciences. All rights reserved.
Author keywords
Adrenal Insufficiency; Karyotype; Lipoid CAH; Neonatal Cholestasi
Strong linkage disequilibrium between -670 A > G and -1377 G > A polymorphisms in FAS gene in patients with breast cancer and controls
No full textBreast cancer is the second cause of mortality in women. The etiology of breast cancer is multi factorial. Genetic factors play an important role in the etimology of breast cancer. The FAS gene, has a critical role in the tumor growth and metastasis. Gene polymorphisms including -1377 G>A and -670 A/G in FAS gene have shown to change the transcription activities of this gene. The FAS genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 115 breast cancer patients, 115 healthy controls, all female and selected from city of Mashhad in north-eastern part of Iran. BstuI and ScrfI were used as endonuclease enzymes to detect -1377G/A and -670A/G gene polymorphisms, respectively. GG, GA and AA genotype frequencies for FAS -1377 polymorphism in patients were 30.4%, 49.6% and 20%, whereas in healthy controls were 26.1%, 50.4% and 23.5%, respectively and there was no significant difference between case and controls (p=0.7).Additionally, genotype frequencies of FAS -670 AA, AG and GG in patient groups were 52.2%, 39.1% and 8.7% respectively . The result of genotype frequency in controls for FAS -670 AA, AG and GG was 47%, 41% and 10.4%, which Showed insignificant difference as compared with patients genotypes (p=0.78). These results showed that FAS−1377G/A and FAS -670 A/G gene polymorphisms had a strong linkage disequilibrium with p value <0.001. These results indicated the lack of association between FAS−1377G>A and FAS -670 A/G gene polymorphisms and risk of breast cancer. Moreover, a significant linkage disequilibrium was found betweenFAS−1377G/A and FAS -670 A/G gene polymorphisms in case and control groups
Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean?
No full textChitinolytic enzymes are capable to catalyze the chitin hydrolysis. Due to their biomedical and biotechnological applications,
nowadays chitinolytic enzymes have attracted worldwide attention. Chitinolytic enzymes have provided numerous useful materials in many different industries, such as food, pharmaceutical, cosmetic, or biomedical industry. Marine enzymes are commonly employed in industry because they display better operational properties than animal, plant, or bacterial homologs. In this
mini-review, we want to describe marine chitinolytic enzymes as versatile enzymes in different biotechnological fields. In this
regard, interesting comments about their biological role, reaction mechanism, production, functional characterization, immobilization, and biotechnological application are shown in this work.Beygmoradi, Azadeh-4357f5bc-fbca-487c-bd3f-f974a9fc2a62-600Homaei, Ahmad-0cc8be4a-ecd4-4ddf-9da7-1c55d129d669-600Hemmati, Roohullah-234501a6-44ca-44cc-8237-20488fcb6269-600Santos-Moriano, Paloma-4ab69e5b-7dab-4c12-a5d6-a1a9493b4384-600Hormigo, Daniel-915adee9-292d-4836-8d92-b79ac158d173-600Fernández-Lucas, Jesús-454d03d3-2cec-45b1-a796-51012f85b786-60
