90 research outputs found
ESPRESSIONE DI SEL1L NEL CARCINOMA MAMMARIO - CORRELAZIONI CLINICO PATOLOGICHE
No full textMalignant cells exhibit an exceptional ability to maintain homeostasis in an hostile environment, upregulation of proteins involved in the stress response with an increase in the apoptosis resistance, and in diminishing cell senescence or permanent arrest of cell division. SEL1L is a member of endoplasmic-reticulum (ER)-associated protein degradation (ERAD)-specific ubiquitin ligase complex that functions in the detection, targeting, and degradation of malfolded and normal endoplasmic reticulum (ER) resident proteins in fisiologic or pathologic conditions. Past studies have focused on the role of SEL1L in various aspects of malignant transformation and tumorigenic processes, providing significant in vitro and in vivo evidences to link its increased expression to a decrease in breast tumor aggressiveness. In this study it has been suggested that SEL1L protein could be also a tumor breast markers with a prognostic and/or predictive response and this may be important in determining the successive therapeutic approach. In patients of Black African ethnicity, breast cancer strongly differ from Western populations in ethnicity, lifestyle and environmental exposures and is reportedly to be characterized by aggressive, poorly differentiated phenotype(s). To highlight possible differences between breast cancer in indigenous sub-Saharan African and European patients, two breast cancer case series, from Central Sudan (Khartoum) and Northern Italy (Milan), were compared for clinicopathological characteristics, expression of oestrogen receptor (ER), progesterone receptor (PR), Her-2/neu, and breast cancer subtypes and for SEL1L expression. SEL1L protein expression was evaluated by immunohistochemistry using the mouse MAb MSel1 raised against the NH2 terminus of the recombinant human SEL1L protein. Compared with the Italian series, the Sudanese breast cancer series showed younger age and worse prognostic features, including larger tumour size, higher grade and LN+ status, consistent with more advanced stage at disease diagnosis.
Results showed that no clear significant differences between the Sudanese and Italian series were found for SEL1L expression levels combined with clinicopathological characteristics, including nodal involvement, tumor size, grade, with hormone receptor status (ER and/or PR), Her-2/neu status and with breast cancer subtypes (Luminal A, Luminal B, Her-2/neu+ and basal-like). The clinicopathological differences between breast cancer in indigenous sub-Saharan African and European patients probably reflects the young demographic structure of the Sudanese population, the lack of breast cancer prevention, the extreme rarity of healthcare centres able to treat breast cancer and, possibly, the influence of socio-cultural and logistic factors which could limit travel and access to healthcare. In conclusion, SEL1L protein expression is not a tumor marker however the role of SEL1L during tumorigenesis and in breast cancer needs further investigation
Sviluppo e implementazione di un sistema semplificato basato su SBTool per la valutazione energetico-ambientale del progetto di edifici residenziali situati nella provincia di Treviso
No full textEvaluation of a multiplex real-time polymerase chain reaction assay for the detection of influenza and respiratory syncytial viruses
No full textNasopharyngeal swabs from 424 children were used to compare the performances of the new multiplex real-time polymerase chain reaction (RT-PCR) RIDA®GENE Flu & RSV kit and monospecific RT-PCR assays in detecting respiratory syncytial and influenza viruses. The easy-to-use kit was highly sensitive and specific and is recommended for routine practice
Identification of Human Adenovirus in Respiratory Samples with Luminex Respiratory Virus Panel Fast V2 Assay and Real-Time Polymerase Chain Reaction
No full textIn order to compare the last version of the Respiratory Virus Panel (RVP) Fast assay for human Adenovirus (hAdv) detection with a specific real-time polymerase chain reaction (qPCR), which is considered the gold standard for hAdv detection, nasopharyngeal samples collected from 309 children (age range, four months to eight years) with respiratory tract infection were tested using the RVP Fast v2 assay (Luminex Molecular Diagnostics, Inc., Toronto, ON, Canada) and a specific TaqMan qPCR to identify hAdv DNA. The RVP Fast v2 assay detected 30/61 (49.2%) hAdv infections that had been identified by real-time qPCR, demonstrating a significantly lower detection rate (p < 0.001). The sensitivity of the RVP Fast v2 assay in comparison to qPCR was lower in younger children (42.9% vs. 57.7%; Cohen's kappa coefficient, 0.53); in samples with co-infections (40.0% vs. 56.7%; Cohen's kappa coefficient, 0.52); and in samples with hAdv type C (45.9% vs. 57.1%; Cohen's kappa coefficient, 0.60). Samples with lower viral loads were associated with a significantly lower sensitivity of the RVP Fast v2 assay (35.1% vs. 68.2%, p = 0.01; Cohen's kappa coefficients, 0.49). The RVP Fast v2 assay has important limitations for the detection of hAdv and cannot be used to evaluate whether hAdvs are the main etiologic agent responsible for an outbreak or when epidemiological studies are performed
Ablazione della Fibrillazione Atriale con Encircling Elettro-anatomico: Esperienza Personale
No full textECHOCARDIOGRAPHIC IDENTIFICATION OF ANOMALOUS ORIGIN OF THE LEFT CIRCUMFLEX CORONARY-ARTERY FROM THE RIGHT SINUS OF VALSALVA
No full textEchocardiographic identification of anomalous origin of the left circumflex coronary artery from the right sinus of Valsalv
Left Atrial Irrigated Tip RF Catheter Ablation of Persistent and Permanent Atrial Fibrillation
No full textVariabilità anatomica delle vene polmonari nei soggetti sottoposti ad ablazione della fibrillazione atriale.
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