1,721,057 research outputs found
Redox proteomics: chemical principles, methodological approaches and biological/biomedical promises
No full text1. Introduction
2. Reactive Oxygen and Nitrogen Species
2.1. Exogenous and Endogenous Cellular Sources of ROS and RNS
2.1.1. Superoxide Anion
2.1.2. Hydrogen Peroxide and Hydroxyl Radical
2.1.3. Nitric Oxide
2.1.4. Peroxynitrite
2.1.5. Derivatives from Lipid Peroxidation
2.2. Molecular Systems Involved in the Maintenance of Cell Redox Homeostasis
2.3. Biological Effects Associated with ROS/RNS Activity
2.4. Oxidative/Nitrosative Damage and Pathological Aspects
3. Proteins as Targets of ROS/RNS Activity
3.1. Cysteine Susceptibility to Redox Reactions
3.1.1. Protein S-Glutathionylation
3.1.2. Protein S-Nitrosylation
3.2. Methionine Sulfoxidation
3.3. Protein Carbonylation
3.4. Tryptophan and Histidine Oxidation
3.5. Tyrosine and Tryptophan Nitration
3.6. Tyrosine Halogenation
4. Proteomic Analysis of Oxidized, Nitrosylated, and Nitrated Proteins
4.1. Proteomic Methods
4.2. Analysis of Protein Adducts Resulting from ROS/RNS-Induced Modifications at Cysteine
4.2.1. Analysis of Protein Adducts Resulting from Disulfide Bond Formation
4.2.2. Analysis of Protein Adducts Resulting from S-Glutathionylation
4.2.3. Analysis of Protein Adducts Resulting from Cysteine Oxidation
4.2.4. Analysis of Protein Adducts Resulting from S-Nitrosylation
4.2.5. The OxMRM Method
4.3. Analysis of Protein Adducts Resulting from ROS/RNS-Induced Modifications at Methionine
4.4. Analysis of Protein Carbonylation Products
4.4.1. Analysis of Protein Adducts Resulting from Oxidation at Lysine, Arginine, Threonine, and Proline
4.4.2. Analysis of Adducts Resulting from Protein Reaction with Lipooxidation End-Products
4.4.3. Analysis of Protein Glycooxidation End-Products
4.5. Analysis of Protein Adducts Resulting from ROS/RNS-Induced Modifications at Tyrosine
4.6. Analysis of Protein Adducts Resulting from ROS/RNS-Induced Modifications at Tryptophan and Histidine
4.6.1. Analysis of Protein Adducts Resulting from Tryptophan Oxidation
4.6.2. Analysis of Protein Adducts Resulting from Tryptophan Nitration
4.6.3. Analysis of Protein Adducts Resulting from Tryptophan Halogenation
4.6.4. Analysis of Protein Adducts Resulting from Histidine Oxidation
5. Concluding Remarks and Future Perspective
Characterization and analysis of D-amino acids.
No full textThe need to screen a large number of natural extracts, with the aim of detecting D-amino acids or isolating and characterizing peptides containing them, has stimulated the development of novel and improved procedures for the analysis of amino acid enantiomeric mixtures, with special attention paid to automation. Different methods for the analysis of D-amino acids are described and discussed
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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