1,721,212 research outputs found
HOOKING TRAIL-R2 TO ¿EDUCATE¿ IMMUNE SYSTEM TO RECOGNIZE CANCER CELLS
Full text linkUse of recombinant human tumor necrosis factor (TNF) related apoptosis inducing ligand (rhTRAIL) or TRAIL-receptors agonistic monoclonal antibodies (mAbs) promotes apoptosis in most cancer cells preserving normal cells. Furthermore, TRAIL-R2, one of the receptor that can trigger cell apoptotic machinery, is expressed at higher levels in tumor tissue as compared to normal tissues. Agonistic mAbs could mimic the tumor killing properties of rhTRAIL, with the advantage to have a prolonged half-life in vivo and to avoid binding of decoy TRAIL-receptors unable to transmit apoptotic signals. Although mAbs have proven to be highly effective, their use still unsolved the problem of TRAIL-resistance that some tumor cells are able to develop. To increase the anti-tumor activity of some mAbs, in last decades, bispecific antibodies (BsAbs) able to redirect cytotoxic activity of tumor resident/ circulating T cells against tumor cells in an MHC-independent fashion have been developed.
In this study, we isolated a BsAb able to bind the CD3, invariant component of the T-cell receptor, and TRAIL-R2. Among the different isolated BsAbs, one in particular presented good thermodynamic characteristics and showed a very good stability even after 2 years its purification. The BsAb was biochemically and functionally well characterized and the production method was optimized. We demonstrated that the BsAb could act with two mechanisms: retargeting T-cells to lyse tumor cells when acting as BsAb and activating apoptotic pathway by triggering TRAIL-R2 when acting as agonistic mAb. T-cell activation was confirmed by up-regulation of CD69 and CD25 and by production of inflammatory cytokines without off target toxicity. In vitro we demonstrated that, after BsAb-mediated retargeting, T cells were able to lyse tumors of different histotypes, like melanoma, breast and ovarian cancer while sparing normal cells
Influenza della rotazione e della curvatura della linea di corrente sullo strato limite turbolento in giranti con pale comunque curve
No full textCalcolo del flusso viscoso sulle pale delle giranti di turbomacchine radiali (Calculation of compressible boundary layer on radial rotating blades)
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
THE DESIGN OF A RADIAL TURBINE FOR MICROGASTURBINE APPLICATIONS
No full textSviluppo di una procedura per il progetto di turbomacchine radiali basata sull'uso di tecniche CF
Analyse expérimentale de l'écoulement à débit partiel dans un modèle en air de pompe-turbine fonctionnant en pompe (Experimental analysis of reduced flow rates in an air model of a pump-turbine operating as a pump)
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Prove in aria su un modello di macchina idraulica reversibile nel funzionamento come pompa
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