688 research outputs found
Simplified HPLC-UV method for the determination of alfa-tocopherol in plasma.
No full textVitamin E, known for its great nutritional importance, is normally included in animal diets as DL-tocopherol acetate.
The authors propose a method that makes it possible to determine the concentration of vitamin E in plasma without
saponification. This method enable to avoid aggressive treatments on the analyte and complex procedures; it detects
vitamin E only in form of DL-x-tocopherol.
Lipoproteins of analysed plasma were denaturised by methanol. Vitamin E was extracted by petroleum ether in presence
of NaCl. The extract was dried by rotavapor at 45 °C, solubilized by methanol and injected in HPLC (C18 column, reversed
phase). The quantitative determination was carried out by UV detector settled on 294 nm.
Tests of repeatability inter-analysis and intra-analysis gave coefficient of variability (CV%) respectively of 1.64 and
2.41%. The mean recovery was 100%
Author response: Alterations of functional connectivity of the motor cortex in Fabry disease: An RS-fMRI study
No full textEFFETTI DELL’ALIMENTAZIONE SULLA ATTIVITA’ PRODUTTIVA DI GALLI APPARTENENTI A DUE LINEE GENETICHE DIVERSE: PRIMI RISULTATI
No full textObstructive oligospermia : the role of interventional radiology in its diagnosis and treatment
No full textPurpose: According to the latest World Health Organization guidelines (2010), oligo-sperm it is due to a sperm concentration of less than 15 million/ml of seminal fluid. The cause can be obstructive and non-obstructive. Interventional radiology allows diagnosis and, in some cases, minimally invasive treatment. Case presentation: A 28-year-old man with oligospermia (7 million/ml of seminal fluid), surgically treated 2 years ago for clinical grade III bilateral varicocele (according to Dubin’s classification), was admitted to the Urology Department for suspected accidental surgical ligation of the left vas deferens. The patient underwent several diagnostic tests including phlebography of the left pampiniform plexus, bilateral vesico-deferentography. The steno-occlusion of the ejaculatory ducts was diagnosed, which was resolved through an innovative interventional radiology treatment. Conclusions: Interventional radiology has played a decisive role in the diagnosis and treatment of the causes of male infertility. In our experience, it has considerable potential in the minimally invasive treatment of steno-obstructive pathologies of the vesico-deferential system
L’IMPIEGO DEI SEMI DI PISELLO ESPANSI (PISUM SATIVUM L.) NELL’ALIMENTAZIONE DELLE BOVINE DA LATTE
No full textL’IMPIEGO DEI SEMI DI PISELLO ESPANSI (PISUM SATIVUM L.) NELL’ALIMENTAZIONE DELLE GALLINE OVAIOLE
No full text
Is the distance between mammillary bodies predictive of a thickened third ventricle floor?
No full textThe aim of this study was to correlate intraoperative endoscopic third ventriculostomy (ETV) findings in
hydrocephalic patients with the MR imaging appearance of the mammillary bodies (MBs), the fundamental anatomical
landmarks of the third ventricle floor (TVF) region.
The authors reviewed brain MR images and intraoperative ETV records in 23 patients with hydrocephalus
as well as MR imaging data from 120 randomized control volunteers of various ages to define the normal
intermammillary distance (IMD).
In control volunteers, no measurable IMD (“kissing” configuration) was observed in 91 (85%) of 107
cases, and there was mild MB splitting (mean ± standard deviation, 0.18 ± 0.12 cm) in only 16 cases with age-related
cerebral atrophy. Among the 21 patients with complete MR imaging and ETV data sets, 12 ETV procedures were
hindered by anatomical anomalies such as a thickened TVF or an “upward ballooning” phenomenon. On preoperative
MR imaging in these 12 patients, there was an increased IMD (0.55 ± 0.41 cm) compared with that in the remaining 9
patients (0.27 ± 0.25 cm) who had a normal thin TVF during ETV and in the control group (0.03 ± 0.08 cm). Magnetic
resonance imaging and ETV data concordantly displayed nonsplit MBs in 6 of 9 cases with a thin TVF and split MBs
in 10 of 12 cases with a thick TVF.
The normal configuration of MBs is no measurable IMD, with mild splitting occurring in patients
with age-related brain atrophy. In hydrocephalic patients, a thickened TVF was present almost exclusively with an
increased IMD on preoperative MR imaging and separated MBs on endoscopic viewing. Large retrospective series
are needed to confirm that a preoperative increased IMD is predictive of a thickened TVF during ETV
Endovascular treatment of chronic hemoptysis in patients with pulmonary tuberculosis
No full textBackground: We aimed to demonstrate the safety and efficacy of bronchial artery embolization (BAE) in patients with pulmonary tuberculosis in the planned management of “mild” hemoptysis. This treat-ment, already widely documented and used as a life-saving therapy in an emergency regimen, if properly planned in poorly controlled patients through medical therapy alone, can provide a valid opportunity by reducing the frequency and extent of non-fatal bleeding, but which still worsen the quality of life of these already significantly traumatized patients. Methods: All procedures were conducted through a right common femoral access with a 5 Fr catheter and a 2.7 Fr super-selective catheter coaxial technique of the branches of the bronchial arteries with suspected bleeding sources. Embolizations were performed with 500-700 micron Terumo PVA plastic microparticles. We decided to adopt the following inclusion criteria for the selection of patients to be enrolled: documented diagnosis of pulmonary TB, the presence of at least one bleeding epi-sode that required at least two blood transfusions, evaluation with bronchoscopic examination to ascertain the bronchial origin of bleeding and the affected lobar site, execution of an angio-ct radiological study for the evaluation of the bronchial systemic anatomy as well as the patency of the pulmonary arterial circulation, general hemodynamic compensation and an age of enrollment between 25 and 65 years. Results: All selective embolization interventions demonstrated a technical success of 100% of the total number of patients. 11 out of 12 patients did not show any signs of relapse or complications related to the interventional procedure at a first check-up carried out at 48 hours, instead a fatal massive hemoptysis occurred in only one patient. At the next three-month follow-up, no relapses were documented in all selected patients. Only one patient required a second embolization four months after the first procedure. Conclusions: Radiological-interventional approach in the elective regimen of super-selective embolization of the bronchial arteries (BAE) in the management and control of “mild” hemoptysis in patients with pulmonary tuberculosis not controlled exclusively by medical therapy, according to a strategy systematic of planned intervention and respecting clear and standardized inclusion criteria, represented in our experience a safe and effective procedure, free from significant short and long term complications, especially in well selected patients, which, although not always allows a definitive and stable control of hemoptysis, can in any case significantly limit the risks, also allowing a better planning of the most appropriate therapeutic intervention strategy
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