1,720,970 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Aptamer based-microarraysfor protein detection: a potential tool for diagnostic applications
No full textAptamers are an emerging class of nucleic acid molecules with applications in several fields. They are RNA or single stranded DNA molecules selected for their folding in complex tridimensional structures: their conformation, encoded in their nucleotide sequence, determines the specific interaction with diverse target molecules in solution. Aptamers bind to specific protein with high specificity and affinity, making them attractive alternatives to the commonly used monoclonal antibodies both for therapeutic and diagnostic applications. Besides lower production costs, added advantages over antibodies are their relative ease of isolation and modifications, tailored binding affinity and reversible denaturation, making them suitable candidates for use in detection systems.
We have developed a Sandwich Aptamer Microarray (SAM) for human thrombin detection immobilizing one aptamer on a glass slide (capture aptamer) and using a secondary aptamer for the detection of the analyte. This aptasensor represents a potential diagnostic system for thrombin and exploits two non-overlapping DNA antithrombin aptamers recognizing different exosites of the target protein, TBA1 and TBA2. The 15-mer aptamer TBA1 binds the fibrinogen binding site, whereas the 29-mer aptamer TBA2 binds the heparin binding domain of human thrombin. This distinct recognition pattern allows their use in tandem, since a ternary complex could possibly be formed by simultaneous recognition of thrombin.
To adapt aptamers to a defined solid phase and to a specific detection technique, appropriate chemical modifications must be introduced. In the case of the microarray, immobilization to the solid support and labeling for detection imply precise chemistries that could sensibly alter the aptamer structure. An extensive analysis on the binary complexes formation has been therefore performed in solution by Electrophoretic Mobility Shift Assay (EMSA) to verify the sandwich complex formation by a Supershift assay.
The system validated in solution was finally applied to the solid phase using an appropriate control and two different protocols for detection, resulting in an aptamer-based microarray for recognition of human thrombin with high specificity, using standard microarray slides and a fast and easy protocol. The aptamer-based biosensor could be used to analyze thrombin concentration in pathologic conditions, therefore representing a potential tool for diagnostic application
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
L'artrografia del ginocchio nello studio degli spandimenti posteriori. Tecnica e primi risultati
No full textAppropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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