1,721,042 research outputs found
STUDIO DI TECNOLOGIE DI AMPLIFICAZIONE E GENOTIPIZZAZIONE DEL GENOMA SU CAMPIONI DI DNA PROVENIENTI DA SANGUE E DA CELLULE DELLA BOCCA PER APPLICAZIONI IN AMBITO EPIDEMIOLOGICO
Get PDFIn epidemiological studies the amount of biological material available is a limiting factor. Many studies use DNA as biological sample obtained by venipuncture, but this collection method is invasive especially if donors are children and the elderly. The use of mouth cells can be an alternative source, although you get DNA of poor quality and quantity. To increase the amount of DNA extracted from buccal cells, you can use the "Whole Genome Amplification”. The aim of my PhD project was to develop a method to extract DNA from buccal cells and to study amplification technologies and subsequent genotyping of DNA extracted from blood and buccal cells. The accuracy of WGA was evaluated with different techniques of molecular biology and genotyping: direct sequencing, allelic discrimination assays, microsatellite genotyping and ”genome wide analysis”. Our analysis showed that the WGA can be used to increase the amount of starting biological material, however, it has some limitations, the fact that direct sequencing and analysis with microsatellites in some cases, may cause a loss of 'genetic information’. According to the data found using DNA from buccal cells and WGA, we have genotyped GSTP1 gene polymorphism Ile105/Val105 about 103 people in the Milan area through Real Time. The study of allele frequencies of this polymorphism in the GSTP1 gene is part of a project aiming to determine whether in patients with respiratory diseases there is an interaction between individual genetic predisposition and exposure to a common external agent coming from urban pollution. The genotypic frequencies obtained in our population were not significantly different from those of Tuscany population genotyped for the HapMap project, so our samples will be used as reference for future studies. Furthermore, we showed that both buccal cells and the WGA can be used in epidemiological analysis for genotyping through Real Time PCR. WGA may be a useful way to increase the amount of DNA; DNA extracted from buccal cells can be a valuable resource for genetic studies
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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