1,720,980 research outputs found
Stability of neuroactive amino acids in cerebrospinal fluid under various conditions of processing and storage.
No full text
Changes in membrane lipid composition in marine species of Euplotes adapted to different thermal environments
No full textCell integrity and activities are largely influenced by membrane fluidity, which is temperature-dependent. Below the so-called transition temperature, phospholipids mobility is drastically reduced. Therefore, life adaptation to extreme environments is supposed to involve significant variations in membrane lipids.
We determined the phospholipid composition in three ecologically separated species of Euplotes: one (E. raikovi) from temperate areas, one (E. focardii) endemic to Antarctic, and one (E. polaris) colonizer of both Arctic and Antarctic.
Lipids were extracted from cell cultures and analyzed by RPLC-ESI-QqQ-MS in positive mode. E. polaris and E. raikovi contained several species of phosphocholines (PC), lyso-PC (L-PC), phosphatidylethanol-amines (PE), lyso-PE, sphingomyelins (SM), phosphatydilinositol (PI) and phosphatidylglycerol (PG). E. focardii contained all the classes but PE and SM. Very interestingly, E. focardii contained the lowest number of lipid species and showed a greater unsaturation index, while total PE of E. polaris were highly unsaturated compared to PE the other two species
Biological variability of myoglobin in healthy elderly and younger subjects
No full textTo study the effect of age on serum myoglobin more clearly, the analytical, intra-individual and inter-individual components of variation were estimated from duplicate analyses of specimens collected from 18 healthy elderly subjects [ages 74-97 years; 9 men (EM)], and 14 healthy younger subjects [ages 25-31 years; 7 men (YM)] over a period of 6 weeks. The mean values (μg/L) were EM: 53.7; EW: 44.9; YM: 34.2; YW:24.8. Estimated analytical (CV(A)), intra- (CV(I)) and inter-individual (CV(G)) variations as CV% were: CV(A): 2.2. CV(I): EM: 13; EW: 9.9; YM: 12.4; YW: 9.6. CV(G): EM: 37.6; EW: 28; YM: 18.5; YW: 13.4. The data obtained were used to derive the desirable analytical goal for imprecision (i.e., ≤6.5% in EM; ≤4.9% in EW and ≤6.2% in YM; ≤4.8% in YW); inaccuracy (i.e., ≤9.9% in EM; ≤7.7 in EW and ≤5.5% in YM; ≤4.12% in YW); the change required for serial results to be significantly different (i.e., 36% in EM; 28% in EW and 34% in YM; 27.2% in YW), the numbers of specimen collections required to produce a more precise estimate of the homeostatic set point of an individual within 5% (i.e., 26 in EM; 16 in EW and 24 in YM; 15 in YW), and the index of individuality (i.e., 0.34 in EM; 0.35 in EW and 0.67 in YM; 0.71 in YW). This study shows that intra-individual biological variation of myoglobin in healthy elderly subjects is not different from that in young subjects. Inter-individual variation, instead, is greatly influenced by differences in age and sex. (C) 2000, Editrice Kurtis
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
- …
