1,743,562 research outputs found
Synthesis of 9-deoxo-9a-aza-9a-homoerythromycin A 11,12-Hydrogen Borate and Azithromycin 11,12-Hydrogen Borate. A New Procedure to Obtain Azithromycin Dihydrate
No full textSynthesis of
9-deoxo-9a-aza-9a-homoerythromycin A
11,12-Hydrogen Borate and Azithromycin
11,12-Hydrogen Borate. A New Procedure
to Obtain Azithromycin Dihydrat
CircRNA-407 sponged miR-9a-5p to regulate ovarian development.
No full text(A) The putative miR-9a-5p binding site in circRNA-407 was predicted by RNAhybrid. (B) Schematic diagram of dual-luciferase reporter gene structure based on pmir-GLO plasmid. The putative binding sites (WT-circRNA-407) and the mutant version (MUT-circRNA-407) for circRNA-407 was presented. (C) Relative luciferase activity was determined 48 h after co-transfecting HEK293T cells with miR-9a-5p/NC-mimics (n = 3) and circRNA-407-WT/MUT (n = 3). Relative luciferase activity in NC-mimics groups was set as 1. (D) FISH assay analysis for the co-localization of miR-9a-5p and circRNA-407 in C6/36 cells. Nuclei was counterstained with DAPI (blue), probes for circRNA-407 was cy3-labeled (red) and probes for miR-9a-5p was FAM-labeled (green). (E) Relative expression level of miR-9a-5p in female mosquitoes upon thoracic injection of miR-9a-5p mimics (OE), negative control mimics (NC) and wild type blank control (WT) determined by qRT-PCR. qRT-PCR reactions were performed triplicates with three biological replicates (n = 15 females per replicate). (F) Representative images of ovaries dissected from miR-9a-5p mimics treated, NC-mimics and WT female mosquitoes at 48 h PBM. All the images were taken with a Nikon SMZ1000 stereomicroscope with DIGITAL SIGHT DS-U3 (Scale bar: 0.5 μm). (G) and (H) Average follicle size (length of the long axis) (G) of ovaries isolated from female mosquitoes and number of developing follicles (H) per female individual in miR-9a-5p mimics treated (n = 45) NC-mimics (n = 45) and WT (n = 45) mosquitoes. All data was shown as mean ± SEM. The Student’s t-test was used to compare the means between two groups and one-way ANOVA was used to compare the means among different groups. ****p < 0.0001; N.S., no significance.</p
Electrochemical Synthesis, Structure Elucidation and Antibacterial Evaluation of 9a-aza-9a-chloro-9a-homoerythromycin A
No full textElectrochemical synthesis, structure elucidation and antibacterial evaluation of 9a-aza-9a-chloro-9a-homoerythromycin A were carried out. It was found that the anodic oxidation of 9a-aza-9a-homoerythromycin A via electrogenerated reactive chlorine species leads to the chlorination of lactam nitrogen in high yield provided the pH of the reaction mixture is maintained above 3. NOESY spectra reveal the existence of the mixture of two conformational families in the solution, the "folding-out" conformer being slightly more abundant comparing to 9a-Aza-9a-homoerythromycin A. The chlorine substitution of lactam hydrogen resulted in improved antimicrobial potency against Sreptococcus pyogenes PSCB0542, Moraxella catarrhalis ATCC 25238, Haemophilus influenzae ATCC 49247 and Enteroccocus faecalis ATCC 29212
A Transcription of the Gospel according to John in Codex Fossatensis (Vetus Latina 9A)
No full textA Transcription of the Gospel according to John in Codex Fossatensis (Vetus Latina 9A)
Russian National Library, St Petersburg, F.v.I.8 Copied in England in the second half of the eighth century
9a Rassegna Biennale d'Architettura 6° Premio Marcello D'Olivo
No full textSi tratta del catalogo della 9a Rassegna Biennale d'Architettura - 6° Premio Marcello d'Olivo - Architetture in Friuli Venezia Giulia 201
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Alternative Exon 9-Encoded Relay Domains Affect More than One Communication Pathway in the Drosophila Myosin Head
No full textWe investigated the biochemical and biophysical properties of one of the four alternative regions within the Drosophila myosin catalytic domain: the relay domain encoded by exon 9. This domain of the myosin head transmits conformational changes in the nucleotide-binding pocket to the converter domain, which is crucial to coupling catalytic activity with mechanical movement of the lever arm. To study the function of this region, we used chimeric myosins (IFI-9b and EMB-9a), which were generated by exchange of the exon 9-encoded domains between the native embryonic body wall (EMB) and indirect flight muscle isoforms (IFI). Kinetic measurements show that exchange of the exon 9-encoded region alters the kinetic properties of the myosin S1 head. This is reflected in reduced values for ATP-induced actomyosin dissociation rate constant (K(1)k(+2)) and ADP affinity (K(AD)), measured for the chimeric constructs IFI-9b and EMB-9a, compared to wild-type IFI and EMB values. Homology models indicate that, in addition to affecting the communication pathway between the nucleotide-binding pocket and the converter domain, exchange of the relay domains between IFI and EMB affects the communication pathway between the nucleotide-binding pocket and the actin-binding site in the lower 50-kDa domain (loop 2). These results suggest an important role of the relay domain in the regulation of actomyosin cross-bridge kinetics
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