1,723,059 research outputs found
Therapeutic Potential of Dioscorea Extract (DA-9801) in Comparison with Alpha Lipoic Acid on the Peripheral Nerves in Experimental Diabetes
Full text linkDA-9801, a mixture of extracts from Dioscorea japonica Thunb. and Dioscorea nipponica Makino, was reported to have neurotrophic activity. Therefore, we investigated the therapeutic potential of DA-9801, in comparison with alpha lipoic acid (ALA), for peripheral nerves preservation in experimental diabetes. Experimental animals were divided into 4 groups, and each group was designated according to the type of treatment administered as follows: normal, DM, DM+DA-9801, and DM+ALA. After 16 weeks, response thresholds to tactile and thermal stimuli were higher in DM+DA-9801 group than in nontreated DM group. This degree of increase in DM+DA-9801 group indicates more therapeutic potency of DA-9801 than ALA. Western blot analysis showed more significant increase in NGF and decrease in TNF-α and IL-6 in DM+DA-9801 group than in DM or DM+ALA groups (P<0.05). IENF density was reduced less significantly in the DM+DA-9801 group than in other DM groups (7.61 ± 0.32, 4.2 ± 0.26, and 6.5 ± 0.30 in DM+DA-9801, DM, and DM+ALA, resp., P<0.05). Mean myelinated axonal area in the sciatic nerves was significantly greater in DM+DA-9801 group than in other DM groups (69.2 ± 5.76, 54.0 ± 6.32, and 63.1 ± 5.41 in DM+DA-9801, DM, and DM+ALA, resp., P<0.05). Results of this study demonstrated potential therapeutic applications of DA-9801 for the treatment of diabetic peripheral neuropathy
Medical doctors' perceptions of the role of clinical pharmacists in Serbian hospitals
No full text41st ESCP symposium on clinical pharmacy: personalised and safe therapy
Barcelona, Spain. 29–31 October 2012Abstracts: [https://doi.org/10.1007/s11096-013-9801-0
P2X1 Receptor-Mediated Ca<sup>2+</sup> Influx Triggered by DA-9801 Potentiates Nerve Growth Factor-Induced Neurite Outgrowth
No full textNerve growth factor (NGF)-induced
neuronal regeneration has emerged
as a strategy to treat neuronal degeneration-associated disorders.
However, direct NGF administration is limited by the occurrence of
adverse effects at high doses of NGF. Therefore, development of a
therapeutic strategy to promote the NGF trophic effect is required.
In view of the lack of understanding of the mechanism for potentiating
the NGF effect, this study investigated molecular targets of DA-9801,
a well-standardized Dioscorea rhizome extract, which
has a promoting effect on NGF. An increase in intracellular calcium
ion level was induced by DA-9801, and chelation of extracellular calcium
ions with ethylene-bis(oxyethylenenitrilo)tetraacetic acid (EGTA)
suppressed the potentiating effect of DA-9801 on NGF-induced neurite
outgrowth. In addition, EGTA treatment reduced the DA-9801-induced
phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2),
the major mediators of neurite outgrowth. To find which calcium ion-permeable
channel contributes to the calcium ion influx induced by DA-9801,
we treated PC12 cells with various inhibitors of calcium ion-permeable
channels. NF449, a P2X1 receptor selective antagonist, significantly
abolished the potentiating effect of DA-9801 on NGF-induced neurite
outgrowth and abrogated the DA-9801-induced ERK1/2 phosphorylation.
In addition, transfection with siRNA of P2X1 receptor significantly
reduced the DA-9801-enhanced neurite outgrowth. In conclusion, calcium
ion influx through P2X1 receptor mediated the promoting effect of
DA-9801 on NGF-induced neurite outgrowth via ERK1/2 phosphorylation
Type of labor in singleton pregnancies: cost-effectiveness analysis
No full text41st ESCP symposium on clinical pharmacy: personalised and safe therapy, Barcelona, Spain. 29–31 October 2012Abstracts: [https://doi.org/10.1007/s11096-013-9801-0
The influence of age, gender and comedication on lamotrigine clearance in patients with epilepsy
No full text41st ESCP symposium on clinical pharmacy:
personalised and safe therapy
Barcelona, Spain. 29–31 October 2012Abstracts: [https://doi.org/10.1007/s11096-013-9801-0
Effects of DA-9801 on the inflammation and apoptosis induced by angiotensin II in human dermal microvascular endothelial cells
No full textDA-9801, a plant-based drug used for the treatment of diabetic neuropathy, is known to improve angiotensin II (Ang II)-induced vascular endothelial cell dysfunction. However, the underlying mechanism is not fully understood. We aimed to determine whether the protective effect of DA-9801 against Ang II-induced endothelial cell dysfunction was mediated via inhibition of endothelial cell inflammation and apoptosis. Ang II-induced oxidative stress was attenuated by pretreatment of human dermal microvascular endothelial cells (HDMECs) with DA-9801. This prevented the Ang II-induced upregulation of NAD(P)H oxidase (the NOX4 and p22phox subunits) and reactive oxygen species. Further, pretreatment of HDMECs with DA-9801 ameliorated Ang II-mediated nuclear factor kappa B activity via prevention of the upregulation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase. It also decreased the Ang II-stimulated increase in inducible nitric oxide synthase (NOS) and decreased endothelial NOS protein expression. DA-9801 decreased Ang II-induced upregulation of intercellular adhesion molecule 1, vascular adhesion molecule, and E-selectin in HDMECs. Moreover, TUNEL and annexin V-FITC fluorescence staining for apoptosis and the activities of caspases 9, 7, and 3 decreased in HDMECs pretreated with DA-9801, indicating that the drug enhanced anti-apoptotic pathways. Thus, DA-9801 modulated Ang II-induced endothelial cell dysfunction via inflammatory and apoptotic pathways
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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