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Interactions between different corneal proteoglycans
Full text linkProteoglycans were extracted from bovine cornea with 4M-guanidinium chloride and
purified by CsCl-density-gradient centrifugation. Under associative conditions two
fractions were found: one capable of forming assemblies of high molecular weight and
another lacking this property. The heavier fraction (density 1.59g/ml) was eluted as a
single retarded peak from Sepharose 2B, but on DEAE-Sephadex chromatography,
gave two peaks: the first (eluted with 0.75 M-NaCI) contained mainly proteochondroitin
sulphate and the second (eluted with 1.25 M-NaCI) mainly proteokeratan sulphate. Each
of these proteoglycans was more retarded on Sepharose 2B than was the original sample
from density-gradient centrifugation. Re-aggregation was obtained by recombination of
the two fractions. The lighter fraction (density 1.44g/ml), containing predominantly
keratan sulphate chains, was eluted from DEAE-Sephadex as a single peak with 1.25 MNaCl
and was retarded on Sepharose 2B: this fraction was not able to form aggregates with
proteochondroitin sulphate. Chemical analyses of the carbohydrate and protein moieties
of the proteoglycans from DEAE-Sephadex confirmed that, in the cornea, different
subunits are present with characteristic aggregation properties and hydrodynamic
volumes
Grammar [935]
No full textGrammar.
This manuscript is now IO Islamic B20 in the India Office collections.
[metadata: Otto Loth, A Catalogue of the Arabic Manuscripts in the Library of the India Office, (volume 1), no. 935 here with further notations and hyperlinks].
935.
B 20. Size 101/2 in. by 53/4 in.; foll. 184. Twenty-five lines in a page.
Another copy of the same Glosses, concluding as no. 932.
Well written, of the eleventh century. Notes at the beginning. The lower part of the MS. is much injured by insects
Santa Fe (ATSF) 935
No full textA photograph print of Santa Fe (ATSF) 935, 2-10-2 (BLW, class 900), Deming, NM
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Legislative testimony regarding SB 935
Full text linkTestimony SB 935, Sacramento, Californi
EC64-935 Yeast Rolls
Full text linkExtension Circular 64-935 This circular is about yeast rolls. It has recipes, methods, and care methods for after baking
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