1,738,485 research outputs found
dph04_earlycopepodite_SB-874
No full textTIFF sequence of interaction SB-874 (4 days post-hatch fish targeting an early copepodite)
MicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)
No full textMicroRNAs (miRNAs) regulate osteogenic differentiation and influence osteoporosis (OP). The aim of this study was to determine the potential role of miR-874-3p in OP. The expression levels of miR-874-3p and leptin (LEP) in the femoral neck trabeculae of 35 patients with or without OP were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The effects of miR-874-3p or LEP on the cell proliferation and alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), and osterix (OSX) levels were observed by upregulating miR-874-3p in human bone marrow mesenchymal stem cells (hBMSCs). Additionally, calcium deposition levels were evaluated using alizarin red staining (ARS). Molecular mechanisms of miR-874-3p and LEP underlying the osteogenic differentiation of hBMSCs were also evaluated using bioinformatics analysis, luciferase reporter assays, and RNA pull-down assays. The miR-874-3p levels were significantly lower in the femoral neck trabeculae of patients with OP than those of the control group, while the opposite was observed regarding the levels of LEP. Expression levels of miR-874-3p in hBMSCs were upregulated during osteogenic differentiation, while those of LEP were downregulated. Moreover, miR-874-3p upregulation promoted ALP, RUNX2, OCN, and OSX mRNA expression, cell proliferation, and calcium deposition in hBMSCs. LEP was found to be a target gene of miR-874-3p. Overexpression of LEP inhibited the expression of osteoblast markers and reversed the effect of osteogenic differentiation induced by the upregulation of miR-874-3p. In conclusion, miR-874-3p promoted the proliferation and differentiation of hBMSCs by downregulating the expression of LEP, thus inhibiting OP. Abbreviations : miRNAs: microRNAs; OP: osteoporosis; hBMSCs: human Bone Marrow Mesenchymal stem cells; LEP: leptin; DEGs: differentially expressed genes</p
Additional file 5 of Aberrant miR-874-3p/leptin/EGFR/c-Myc signaling contributes to nasopharyngeal carcinoma pathogenesis
No full textAdditional file 5: Supplementary Figure S5. miR-874-3p suppresses the malignant properties of TW02 cells. (a and b) MTS assays and colony formation assays were performed to assess the cell proliferation of TW02 cells after transfecting miR-874-3p mimics or miR-874-3p inhibitor and their corresponding negative control. The representative images and fold change of foci formation were shown. (c and d) The migratory and invasive abilities of TW02 cells transfected with miR-874-3p mimics or miR-874-3p inhibitor and their corresponding negative control were assessed by wound healing and Transwell assays. *P<0.05, **P<0.01, ***P<0.001
Supplementary Material for: miR-874-3p is identified as a biomarker for acute kidney injury and mediates disease development via targeting MSRB3
No full textIntroduction: Acute kidney injury (AKI) is a common clinical disease, especially in the intensive care unit. Identification of reliable biomarker is of great clinical significance and benefit the therapy and prevention of AKI. The clinical significance and function of miR-874-3p in AKI development were evaluated in this study aiming to explore a novel biomarker for AKI.
Methods: There were 83 AKI patients and 56 healthy individuals included and the serum samples were collected. The AKI animal models were established via ischemic/reperfusion and LPS on C57BL/6 mice. The expression of miR-874-3p was evaluated using PCR, while the potential downstream targets were also validated in AKI mice.
Results: miR-874-3p was downregulated in both AKI patients and established AKI mice models. The downregulation of miR-874-3p could discriminate against AKI patients and predict poor prognosis of patients. miR-874-3p was negatively correlated with the levels of Scr, BUN, CRP, NEU, and PCT and positively correlated with the eGFR of AKI patients. In I/R- and LPS-induced AKI mice, overexpressing miR-874-3p could alleviate renal dysfunction, oxidative stress, and inflammation induced by AKI. Additionally, miR-874-3p could negatively regulate the expression of MSRB3, which was speculated as the potential mechanism underlying the function of miR-874-3p in AKI.
Conclusion: miR-874-3p served as a diagnostic and prognostic biomarker of AKI and mediate the severity and development of AKI via targeting MSRB3
Supplementary Material for: miR-874-3p is identified as a biomarker for acute kidney injury and mediates disease development via targeting MSRB3
No full textIntroduction: Acute kidney injury (AKI) is a common clinical disease, especially in the intensive care unit. Identification of reliable biomarker is of great clinical significance and benefit the therapy and prevention of AKI. The clinical significance and function of miR-874-3p in AKI development were evaluated in this study aiming to explore a novel biomarker for AKI.
Methods: There were 83 AKI patients and 56 healthy individuals included and the serum samples were collected. The AKI animal models were established via ischemic/reperfusion and LPS on C57BL/6 mice. The expression of miR-874-3p was evaluated using PCR, while the potential downstream targets were also validated in AKI mice.
Results: miR-874-3p was downregulated in both AKI patients and established AKI mice models. The downregulation of miR-874-3p could discriminate against AKI patients and predict poor prognosis of patients. miR-874-3p was negatively correlated with the levels of Scr, BUN, CRP, NEU, and PCT and positively correlated with the eGFR of AKI patients. In I/R- and LPS-induced AKI mice, overexpressing miR-874-3p could alleviate renal dysfunction, oxidative stress, and inflammation induced by AKI. Additionally, miR-874-3p could negatively regulate the expression of MSRB3, which was speculated as the potential mechanism underlying the function of miR-874-3p in AKI.
Conclusion: miR-874-3p served as a diagnostic and prognostic biomarker of AKI and mediate the severity and development of AKI via targeting MSRB3
Rhetoric [874]
No full textRhetoric.
This manuscript is now IO Islamic 1459 in the India Office collections.
[metadata: Otto Loth, A Catalogue of the Arabic Manuscripts in the Library of the India Office, (volume 1), no. 874 here with further notations and hyperlinks].
874.
1459.Size 9 in. by 51/4 in.; foll. 279. Twenty-one lines in a page.
Another copy of the preceding Glosses. Well written.
[Hastings.
ALVIN 874 Dive 874 December 18, 1978
No full textALVIN 874 Dive 874 December 18, 1978The runtime for this video is 28m 55s.Alvin dive 874 took place on December 18, 1978 during voyage LU-102. The Chief Scientist was F. Grassle in the operating area of Dos#3. The purposewas described as 'BIOLOGY' and the sponsor as 'J.A.', or Joint Agreement of National Science Foundation, Office of Naval Research, and National Oceanic and Atmospheric Administration.The items in this collection are embargoed in the system in keeping with copyright requirements. However, they are available to users upon request. Please contact the MBLWHOI Library to access the items at [email protected] of this material was supported by a Recordings at Risk grant from the Council on Library and Information Resources (CLIR). The grant program is made possible by funding from The Andrew W. Mellon Foundation. CLIR is an independent, nonprofit organization that forges strategies to enhance research, teaching, and learning environments in collaboration with libraries, cultural institutions, and communities of higher learning. To learn more, visit www.clir.org and follow CLIR on Facebook and Twitter.2200-01-0
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