1,723,829 research outputs found
Coldham-Fussell, H, 8710
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/377938Surname: COLDHAM-FUSSELL
Given Name(s) or Initials: H
Military Service Number or Last Known Location: 8710
Missing, Wounded and Prisoner of War Enquiry Card Index Number: 51351191751
Item: [2016.0049.10232] "Coldham-Fussell, H, 8710
Is the Mitochondrial Membrane Potential (∆Ψ) Correctly Assessed? Intracellular and Intramitochondrial Modifications of the ∆Ψ Probe, Rhodamine 123
No full textThe mitochondrial membrane potential (∆Ψ) is the driving force providing the electrical component of the total transmembrane potential of hydrogen ions generated by proton pumps, which is utilized by the ATP synthase. The role of ∆Ψ is not limited to its role in bioenergetics since it takes part in other important intracellular processes, which leads to the mandatory requirement of the homeostasis of ∆Ψ. Conventionally, ∆Ψ in living cells is estimated by the fluorescence of probes such as rhodamine 123, tetramethylrodamine, etc. However, when assessing the fluorescence, the possibility of the intracellular/intramitochondrial modification of the rhodamine molecule is not taken into account. Such changes were revealed in this work, in which a comparison of normal (astrocytic) and tumor (glioma) cells was conducted. Fluorescent microscopy, flow cytometry, and mass spectrometry revealed significant modifications of rhodamine molecules developing over time, which were prevented by amiodarone apparently due to blocking the release of xenobiotics from the cell and their transformation with the participation of cytochrome P450. Obviously, an important role in these processes is played by the increased retention of rhodamines in tumor cells. Our data require careful evaluation of mitochondrial ∆Ψ potential based on the assessment of the fluorescence of the mitochondrial probe
INVESTIGATING THE MECHANISM OF TWO SYNTHETIC PROTEINS FOUND TO RESCUE E. COLI AUXOTROPHIC STRAINS: ∆GLTA AND ∆FES
No full textAre functional proteins difficult to create in the laboratory from scratch? To probe this question, we created and tested a combinatorial library of de novo proteins in a gene knockout experiment. Bacterial strains lacking genes essential for survival on minimal media were transformed with the library and screened for growth. Six strains including ∆gltA and ∆fes were rescued by de novo sequences. ∆gltA is deficient of the enzyme citrate synthase in the citric acid cycle and ∆fes is deficient of the enzyme enterobactin esterase which is involved in iron acquisition. The mechanisms of rescue of these two auxotrophs by de novo sequences were investigated in this research.
Chapter 2 focuses on the rescue protein for ∆gltA, named synGltA. synGltA was tested for its role in conferring life to a glutamate deficient cell. The sequence was determined to be important, based on alanine mutations, and it was also evolved into a more functional sequence. It was found that the protein was not bypassing the natural reaction of gltA, indeed, the expression of synGltA enabled citrate production. It was also found that purified protein was unable to catalyze citrate production alone, meaning that other proteins were involved in rescue in vivo. Protein partners were investigated for synGltA, and two proteins were found to be necessary for the rescue of ∆gltA by synGltA. Together, these findings lay the groundwork for showing that synGltA, a de novo protein, has a meaningful function in ∆gltA rescue.
Chapter 3 focuses on one of the rescue proteins for ∆fes, named synFes2. synFes2 was probed directly for its ability to enable iron acquisition. It was found that synFes2 expression produced dramatically red cells. This red phenotype was found to be due to an accumulation of ferric enterobactin, a substrate of the wild type fes protein. Testing of different growth conditions and cell variants determined that this accumulation was due to more import of ferric enterobactin. Investigating the function of synFes2 suggested that the protein was not capable of hydrolyzing ferric enterobactin. synFes2 was also not found to significantly bind iron or enterobactin compared to a control. These findings suggest that synFes2 is functioning with other proteins to import more ferric enterobactin
Difluorovinyl Liquid Crystal Diluters Improve the Electro-Optical Properties of High-∆n Liquid Crystal Mixture for AR Displays
No full textA liquid crystal (LC) mixture in liquid crystal on silicon (LCoS) is the core material for augmented reality (AR) displays. However, a LC mixture with high birefringence (Δn) and large dielectric anisotropy (Δε) possesses high viscosity (γ1), which results in a slow response time of LCoS devices for AR displays. This work proposes to apply difluorovinyl-based LC diluters to fine balance the low viscosity, high ∆n, and large ∆ε of the LC mixture for a fast response time. Through studying their effects on the key electro-optical properties of a high-∆n LC mixture, it is found that doping these diluter molecules to a high-∆n LC mixture can decrease the viscoelastic coefficient (γ1/K11), increase ∆ε and the figure of merit, maintain a wide nematic phase temperature range, a high clearing point, and ∆n. It also means that these diluters could effectively regulate the relationship between ∆n, ∆ε, and γ1 in the LC mixtures to achieve a fine balance of various excellent properties and further improve the LC device’s response time. The widespread applications of these liquid crystal diluters in emerging liquid crystal optical devices are foreseeable
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Linked collectors and determiners for: The Oriental stick insect genus Orestes Redtenbacher, 1906: Taxonomical notes and six new species from Vietnam (Phasmida: Heteropterygidae: Dataminae).
No full textNatural history specimen data linked to collectors and determiners held within, "The Oriental stick insect genus Orestes Redtenbacher, 1906: Taxonomical notes and six new species from Vietnam (Phasmida: Heteropterygidae: Dataminae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/4ed337be-e700-480f-8710-8c4b0e89dc38">https://bionomia.net/dataset/4ed337be-e700-480f-8710-8c4b0e89dc38</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/4ed337be-e700-480f-8710-8c4b0e89dc38">https://gbif.org/dataset/4ed337be-e700-480f-8710-8c4b0e89dc38</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: The Oriental stick insect genus Orestes Redtenbacher, 1906: Taxonomical notes and six new species from Vietnam (Phasmida: Heteropterygidae: Dataminae).
No full textNatural history specimen data linked to collectors and determiners held within, "The Oriental stick insect genus Orestes Redtenbacher, 1906: Taxonomical notes and six new species from Vietnam (Phasmida: Heteropterygidae: Dataminae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/4ed337be-e700-480f-8710-8c4b0e89dc38">https://bionomia.net/dataset/4ed337be-e700-480f-8710-8c4b0e89dc38</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/4ed337be-e700-480f-8710-8c4b0e89dc38">https://gbif.org/dataset/4ed337be-e700-480f-8710-8c4b0e89dc38</a>. Formatted as a Frictionless Data package
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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