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Additional file 1 of The complete genome sequence of the nitrile biocatalyst Rhodococcus rhodochrous ATCC BAA-870
No full textAdditional file 1: SuppInfo Frederick et al. BAA-870 genome. Table S1. All sequenced Rhodococcus strains (353) according to the NCBI database (accessed 13/03/2019). Table S2. All complete sequenced Rhodococcus species ranked by release date according to the NCBI Genome database (accessed 11/03/2019). Table S3. Whole genome distance statistics between Rhodococcus rhodochrous ATCC BAA-870 and two closely matched strains. Table S4. Rhodococcus rhodochrous ATCC BAA-870 protein function breakdown based on BASys annotation COG classifications
The complete genome sequence of the nitrile biocatalyst Rhodococcus rhodochrous ATCC BAA-870
No full textBackground: Rhodococci are industrially important soil-dwelling Gram-positive bacteria that are well known for both nitrile hydrolysis and oxidative metabolism of aromatics. Rhodococcus rhodochrous ATCC BAA-870 is capable of metabolising a wide range of aliphatic and aromatic nitriles and amides. The genome of the organism was sequenced and analysed in order to better understand this whole cell biocatalyst.
Results: The genome of R. rhodochrous ATCC BAA-870 is the first Rhodococcus genome fully sequenced using Nanopore sequencing. The circular genome contains 5.9 megabase pairs (Mbp) and includes a 0.53 Mbp linear plasmid, that together encode 7548 predicted protein sequences according to BASys annotation, and 5535 predicted protein sequences according to RAST annotation. The genome contains numerous oxidoreductases, 15 identified antibiotic and secondary metabolite gene clusters, several terpene and nonribosomal peptide synthetase clusters, as well as 6 putative clusters of unknown type. The 0.53 Mbp plasmid encodes 677 predicted genes and contains the nitrile converting gene cluster, including a nitrilase, a low molecular weight nitrile hydratase, and an enantioselective amidase.
Although there are fewer biotechnologically relevant enzymes compared to those found in rhodococci with larger genomes, such as the well-known Rhodococcus jostii RHA1, the abundance of transporters in combination with the myriad of enzymes found in strain BAA-870 might make it more suitable for use in industrially relevant processes than other rhodococci.
Conclusions: The sequence and comprehensive description of the R. rhodochrous ATCC BAA-870 genome will facilitate the additional exploitation of rhodococci for biotechnological applications, as well as enable further characterisation of this model organism. The genome encodes a wide range of enzymes, many with unknown substrate specificities supporting potential applications in biotechnology, including nitrilases, nitrile hydratase, monooxygenases, cytochrome P450s, reductases, proteases, lipases, and transaminases
870 SISPA Corps Cadet Officer receives Second Lieutenant commissioning
No full textKUANTAN, 7 November 2021 – A total of 870 SISPA Corps Cadet Officers from 24 Higher Education Institutions (HEI) and one Institute of Teacher Education (IPG) received commissioning as Junior Lieutenants (PA) in conjunction with the 12th Civil Defence Student Corps (SISPA Corps) National Commissioning Ceremony 2021
The complete genome sequence of the nitrile biocatalyst Rhodocccus rhodochrous ATCC BAA-870
No full textBACKGROUND: Rhodococci are industrially important soil-dwelling Gram-positive bacteria that are well known for both nitrile hydrolysis and oxidative metabolism of aromatics. Rhodococcus rhodochrous ATCC BAA-870 is capable of metabolising a wide range of aliphatic and aromatic nitriles and amides. The genome of the organism was sequenced and analysed in order to better understand this whole cell biocatalyst. RESULTS: The genome of R. rhodochrous ATCC BAA-870 is the first Rhodococcus genome fully sequenced using Nanopore sequencing. The circular genome contains 5.9 megabase pairs (Mbp) and includes a 0.53 Mbp linear plasmid, that together encode 7548 predicted protein sequences according to BASys annotation, and 5535 predicted protein sequences according to RAST annotation. The genome contains numerous oxidoreductases, 15 identified antibiotic and secondary metabolite gene clusters, several terpene and nonribosomal peptide synthetase clusters, as well as 6 putative clusters of unknown type. The 0.53 Mbp plasmid encodes 677 predicted genes and contains the nitrile converting gene cluster, including a nitrilase, a low molecular weight nitrile hydratase, and an enantioselective amidase. Although there are fewer biotechnologically relevant enzymes compared to those found in rhodococci with larger genomes, such as the well-known Rhodococcus jostii RHA1, the abundance of transporters in combination with the myriad of enzymes found in strain BAA-870 might make it more suitable for use in industrially relevant processes than other rhodococci. CONCLUSIONS: The sequence and comprehensive description of the R. rhodochrous ATCC BAA-870 genome will facilitate the additional exploitation of rhodococci for biotechnological applications, as well as enable further characterisation of this model organism. The genome encodes a wide range of enzymes, many with unknown substrate specificities supporting potential applications in biotechnology, including nitrilases, nitrile hydratase, monooxygenases, cytochrome P450s, reductases, proteases, lipases, and transaminases.BT/Industriele MicrobiologieBT/Biocatalysi
870 Pegawai Kadet Kor Sispa terima pentauliahan Leftenan Muda
No full textKUANTAN, 7 November 2021 – Seramai 870 Pegawai Kadet Kor Sispa daripada 24 Institusi Pengajian Tinggi (IPT) dan satu Institusi Pendidikan Guru (IPG) terima pentauliahan sebagai Leftenan Muda (PA) bersempena Majlis Pentauliahan Kor Siswa Siswi Pertahanan Awam (Kor Sispa) Peringkat Kebangsaan Kali ke-12 Tahun 2021
Santa Fe (ATSF) 870
No full textA photograph print showing Santa Fe (ATSF) 870, 2-8-0, class 870. ex: RM&SF 10
Geneza demokratycznych instytucji samorządu na terenach Islandii w latach 870–930
No full text The genesis of the democratic self-government institutions in the Iceland in 870–930The article presents information about creating of the politic institution in Iceland in 870–930. The author’s intention is the study and description of the development of democratic self-governing institutions with demonstration proofs that Iceland self-govern institution can be percepted like democratic. It also indicates the main features of Icelandic society that have left a mark on government institutions. The genesis of the democratic self-government institutions in the Iceland in 870–930The article presents information about creating of the politic institution in Iceland in 870–930. The author’s intention is the study and description of the development of democratic self-governing institutions with demonstration proofs that Iceland self-govern institution can be percepted like democratic. It also indicates the main features of Icelandic society that have left a mark on government institutions
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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