1,724,726 research outputs found
8606
Full text linkN-carbamoyl-β-alanine amidohydrolase (CβAA) constitutes one of the most important groups of industrially relevant enzymes used in the production of optically pure amino acids and derivatives. In this study, a CβAA-encoding gene from Rhizobium radiobacter strain MDC 8606 was cloned and overexpressed in Escherichia coli. The purified recombinant enzyme (RrCβAA) showed a specific activity of 14 U·mg−1 using N-carbamoyl-β-alanine as a substrate with an optimum activity at 55 °C and pH 8.0. In this work, we report also the first prokaryotic CβAA structure at a resolution of 2.0 Å. A discontinuous catalytic domain and a dimerisation domain attached through a flexible hinge region at the domain interface have been revealed. We identify key ligand binding residues, including a conserved glutamic acid (Glu131), histidine (H385) and arginine (Arg291). Our results allowed us to explain the preference of the enzyme for linear carbamoyl substrates, as large and branched carbamoyl substrates cannot fit in the active site of the enzyme. This work envisages the use of RrCβAA from R. radiobacter MDC 8606 for the industrial production of L-α-, L-β- and L-γ-amino acids. The structural analysis provides new insights on enzyme–substrate interaction, which shed light on engineering of CβAAs for high catalytic activity and broad substrate specificity
[USMC Order 8606 to 2nd Brigade in Nicaragua]
No full textUndated radio transcript of an order numbered 8606 from "MARCORPS" to the Second Marine Brigade Commander, ordering the transfer of a Marine detachment aboard USS Camden (AS-6) to 2nd Brigade command in Nicaragua
Linked collectors and determiners for: Phytoplankton community composition in the water column of the West Greenland shelf, July 2021.
No full textNatural history specimen data linked to collectors and determiners held within, "Phytoplankton community composition in the water column of the West Greenland shelf, July 2021". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/6e5fa973-a7cc-4867-8606-8bcaa395f7de">https://bionomia.net/dataset/6e5fa973-a7cc-4867-8606-8bcaa395f7de</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/6e5fa973-a7cc-4867-8606-8bcaa395f7de">https://gbif.org/dataset/6e5fa973-a7cc-4867-8606-8bcaa395f7de</a>. Formatted as a Frictionless Data package
California Poll: 8606 -- October 27-30, 1986
No full textThe Field Poll, established in 1945 as The California Poll by Mervin Field, has operated continuously as an independent, non-partisan, media-sponsored public opinion news service. Each year the Field Polls cover a wide range of political and social topics examining California public opinion. Continuing measures are made of voter support for leading political figures vying for major state and federal offices, job ratings of important political figures and reactions to significant political events. Voter awareness, understanding and predispositions of major campaign issues and salient statewide ballot propositions are also tracked over time. For poll 8606 N=701
Shelly rail studies at University of Illinois Oct 1962
No full textA continuation of earlier studies on shelly rail testing at the University of Illinois on various types of rail.Made available in DSpace on 2021-11-04T16:27:46Z (GMT). No. of bitstreams: 2
TAM629-UILU-ENG-1971-8606.pdf: 22082085 bytes, checksum: 964a9f20185f41ddb3c13b26e8c029af (MD5)
license.txt: 4802 bytes, checksum: 58353f9dd6876860dd5221f3d7872a95 (MD5)
Previous issue date: 1962-10Association of American Railroads 62/1
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
