1,723,120 research outputs found
General Medical Corporation at 8505 Adamo Dr
No full textThe General Medical Corporation building front located at 8505 Adamo Drive in Tampa, Florida.https://digitalcommons.usf.edu/gandy_street/1150/thumbnail.jp
SATA-8505 Does Not Impact USA300 Growth in Human Blood.
No full text<p>(a) Hourly quantification of starting culture of 10<sup>6</sup> CFU USA300 in three parts TSB and one part whole blood from healthy donor or a patient with CGD, grown with or without 10<sup>8</sup> PFU SATA-8505 in duplicate. (b) Regrowth of surviving colonies from whole blood culture run in triplicate. One colony of <i>S</i>. <i>aureus</i> previously grown in 3:1 TSB:Whole blood without phage was subsequently grown in TSB with SATA-8505 (TSB/Phage) or without phage (TSB/TSB); one colony of the surviving <i>S</i>. <i>aureus</i> previously grown in the presence of SATA-8505 was subsequently grown in TSB with SATA-8505 (Phage/Phage) or without phage (Phage/TSB). (c) Average quantification of starting culture of 10<sup>10</sup> CFU of USA300 grown in either 3:1 TSB:Whole blood or 3:1 TSB:HBSS, with or without 10<sup>7</sup> PFU SATA-8505. (d) Average quantification of starting culture of 10<sup>7</sup> CFU of USA300 grown in three parts TSB with either one part whole blood, HBSS, serum, or peripheral mononuclear cells (PBMC) in equivalent volume HBSS. Data shown are representative of 2–3 independent experiments using 3 or more different healthy volunteers and 2 patients with CGD. Data is displayed as mean + s.e.m. ** = p<0.01.</p
SATA-8505 Exhibits Commercial Viability but Significant Strain Limitations.
No full text<p>(a) SATA-8505 quantified over up to sixty days after storage at either room temperature (RT), 4 degrees Celsius (4<sup>°</sup>C), or frozen at -80<sup>°</sup>C. (b) Average hourly quantification of 10<sup>8</sup>–10<sup>9</sup> CFU of USA100 <i>S</i>. <i>aureus</i> or a vancomycin resistant variant of USA100 (VRSA) cultured with up to 10<sup>11</sup> PFU of SATA-8505. Data shown are a representative of 2 independent experiments.</p
Naval Postgraduate School Hobby Computer Club / Newsletter No. 8505 [8507]
Full text linkPublication is undated; date estimated from issue number. This issue is marked "Newsletter No. 8505" but appears to be from July
SATA 8505 Effectively Kills USA 300 and Reduces its Viability in vitro.
No full text<p>(a) Average colony forming unit (CFU) counts for USA300 cultured with SATA-8505 for up to four hours at ratios of <i>S</i>. <i>aureus</i>:Phage of 1:1, 1:10, or 1:100 (MOI 1, 10, 100 respectively). (b) Images of surviving colony morphology of USA300 grown in TSB after exposure to BHI (diluent) or SATA-8505. (c) Regrowth of surviving colonies pictured in panel b, <i>S</i>. <i>aureus</i> grown in TSB after prior exposure to BHI (diluent) or SATA-8505 run in triplicate culture. Data shown are representative of 3 or more independent experiments and displayed as mean <u>+</u> s.e.m. **** = p<0.0001.</p
The Identification of the Tomb Described on O. BM 8505
No full textThe tomb described on O. BM 8505 is generally believed to be KV 16, tomb of Ramses I. However, a precise reconstruction of the architectural elements described by the text and comparison with the modern surveys carried out by the Theban Mapping Project in the Valley of the Kings and the Valley of the Queens suggest instead that the tomb described on this ostracon is QV 51, tomb of queen Isis, built under Ramses VI
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
SATA-8505 Induces Interferon Gamma in Primary Human Keratinocytes.
No full text<p>Human peripheral mononuclear cells (PBMC) were cultured in triplicate at 2<sup>6</sup>/mL with SATA-8505 at 1 PFU/mL to 10<sup>8</sup> PFU/mL at ten-fold increments or diluent derived from the supernatant of an overnight culture of SA that had been pelleted and filter sterilized in a manner similar to the phage-containing media. At 72 hours supernatants were harvested and analyzed for IL-1ß (a), IL-6 (b), IL-17A (c), and IFN gamma (d). Human keratinocytes from primary foreskins (foreskin keratinocytes; FSKC) or the HaCaT cell line were cultured to confluence on 6-well plates and incubated in triplicate with SATA-8505 at 10<sup>4</sup> PFU/mL to 10<sup>8</sup> PFU/mL at ten-fold increments or TSB diluent. At 24 hours supernatants were harvested and analyzed for IL-1ß (e), IL-6 (f), and IFN gamma (g). Phage for all experiments was diluted in TSB from overnight culture of USA300 that was centrifuged at 5000rpm for 12 minutes and filter-sterilized through a 0.44 micrometer filter. Data shown are representative of 3 independent experiments using 3 different healthy volunteers (a-d) or a pool of 5 or more foreskin samples (e-g) and displayed as mean <u>+</u> s.e.m. * = p <0.05.</p
SATA-8505 Improves MRSA Skin Infection at Low MOI.
No full text<p>Mice were injected intraperitoneally (I.P.) with 10<sup>7</sup> plaque-forming units (PFU) of SATA-8505 immediately prior to subcutaneous injections of 10<sup>7</sup> CFU of USA300. (a) Lesion size progression over following six days. On day 6, skin biopsies were homogenized for culture, total bacterial (b) and phage burden (c) were calculated for individual mice. (d) mRNA transcript levels for IL-1ß, IL-6, and IL-17A in individual CGD mice relative to wild type controls, standardized to GAPDH. (e-g) mRNA transcript levels for IL-1ß, IL-6, and IL-17A in CGD and wild type mice with and without SATA-8505 treatment relative to wild type controls injected with diluent, standardized to GAPDH. Data shown are representative of 2–3 independent experiments using 5 or more mice per group, and displayed as mean + s.e.m. Differences were calculated by ANOVA with Bonferroni correction and depict differences from diluent treated wild type unless otherwise noted. ns = not significant, * = p <0.05, ** = p<0.01.</p
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