1,722,965 research outputs found
Chemosensitivity of human hepatocellular carcinoma cell line QGY-7703 is related to Bcl-2 protein levels
No full textBcl-2 protein is one of the major apoptosis regulators. The study examines the effect of Bcl-2 protein on the chemosensitivity of a human hepatocellular carcinoma cell line, QGY-7703, Western blot analysis showed that Bcl-2 and Bax proteins were expressed in QGY-7703 cells. Characteristic features of Taxol- and doxorubicin-induced apoptosis were evidenced by the Annexin-V binding assay, TUNEL and DAPI staining. At constant Bax protein levels, stable sense and antisense gene-transfected QGY-7703 cells showed that constitutive expression of Bcl-2 could render the cells more resistant to Taxol and doxorubicin. Contrarily, decreased Bcl-2 levels caused the cells to be more sensitive to the drugs. As Bcl-2 levels are directly proportional to the resistance of QGY-7703 cells to Taxol and doxorubicin, manipulation of Bcl-2 could be performed to enhance the sensitivity of liver cancer to chemotherapeutic agents.</p
Archaeobotanical remains from Mitchelstown and Ballynamona, Middle/Late Bronze Age house sites in Cork, Ireland
No full text.pdf file with report describing analysis of archaeobotanical material from Ballynamona 2 and Mitchelstown 1 in north Cork, Ireland. As a result of peer review, this file supersedes that found at DOI:10.5281/zenodo.7703 (https://zenodo.org/record/7703#.UuUb2NJFDwc). The modifications are monir (a change of the colour in one graph)
[The Texas & Pacific Railway Co., Engine Drawing Card, Sketch No. 7703]
No full textThis engine drawing card was created for Texas & Pacific Railway, Class 12-48 1/4D. Section J-12 1/4D. Sketch 7703. Copy Spec. [field left blank]
Ringtestsamarbeid for kjemiske vannanalyser. Ringtest 7703: Aluminium, bly, jern, kadmium, kobber, krom, mangan, nikkel og sink
No full textSom ledd i et løpende ringtestsamarbeid for kjemiske vannanalyser ble ringtest 7703 arrangert i juni 1977 med 94 deltakende laboratorier. Ringtesten omfattet bestemmelse av aluminium, bly, jern, kadmium, kobber, krom, mangan, nikkel og sink, og ble gjennomført etter youdens metode
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
mRNA expression and protein level of <i>MTHFR</i> in QGY-7703 cells under conditions of FA deficiency (7<sup>th</sup>, 14<sup>th</sup> and 21<sup>st</sup> day); GAPDH served as an internal reference.
No full text(a) RT-qPCR assays revealed the mRNA expression of MTHFR in QGY-7703 cells under conditions of FA deficiency on the 7th day. (b) RT-qPCR assays revealed the mRNA expression of MTHFR in QGY-7703 cells under conditions of FA deficiency on the 14th day. (c) RT-qPCR assays reveal the mRNA expression of MTHFR in QGY-7703 cells under conditions of FA deficiency on the 21st day. (d, e) Western blot assays showed the relative protein expression of MTHFR in QGY-7703 cells under conditions of FA deficiency (7th, 14th, 21st day); GAPDH served as an internal reference.</p
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Promotions of cell proliferation and invasion by Fibulin-3 siRNA in QGY-7703 cells.
No full text<p>A. Fibulin-3 siRNA noticeably downregulated expression of Fibulin-3 protein. Scramble and Fibulin-3 siRNA were transfected into QGY-7703 cells for 24 h. Relative Fibulin-3 expressions were detected by western blot. B. Fibulin-3 siRNA significantly increased cell viability in HCC cells. Cells transfected with scramble siRNA (ss) and Fibulin-3 siRNA (#1 and #2) were seeded into 96-well plates and cultured for 24 h. The cell viabilities were determined using MTT assays. The data are means ± SD of three independent experiments, *<i>P</i><0.05. C. Fibulin-3 siRNA promoted cell invasion <i>in vitro</i>. QGY-7703 cells were seeded into transwell chambers coated with Matrigel. After 24 h, invaded cells stained by 0.1% crystal violet were counted. The data are means ± SD of three independent experiments, *<i>P</i><0.05.</p
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