1,748,950 research outputs found
Trastuzumab treatment together with miR-770-5p modulates AKT and ERK expression level.
No full textmiR-770-5p mimic was transfected to BT-474 cells and; total and phoshorylated AKT and ERK protein levels were analyzed by Western blot 72 h after transfection. Combination of trastuzumab with miR-770-5p mostly affected pERK and pAKT levels in BT-474 cells (n = 3, *p<0.05).</p
miR-770-5p overexpression downregulated HER2 and increased the effect of trastuzumab.
No full text(a) The protein expression level of HER2 was slightly diminished in both cell lines following miR-770-5p transfection n = 3, p(b) Combinational treatment of cells with trastuzumab and miR-770-5p decreased cell viability in BT-474 and SK-BR-3 cells (n = 3, *p(c) (**p<0.05).</p
Pathway enrichment analysis results conducted with the targets of miR-770-5p.
No full textPathway enrichment analysis results conducted with the targets of miR-770-5p.</p
Lower expression of miR-770-5p is associated with tumor samples.
No full textExpression level of miR-770-5p was downregulated in tumor samples compared to normal samples (One-way ANOVA; p<0.00005621, f = 10.44).</p
miR-770-5p regulates motility and invasion in BT-474 and SK-BR-3 cells.
No full text(a) The rate of motility was assessed by wound-healing assay. Wound closure was observed in scrambled control-transfected cells in a time-dependent manner while miR-770-5p mimic transfected cells lost their motility (n = 2, *p(b) Cell invasion analysis was performed by xCELLigence real-time cell analyzer measuring impedance-based signals. Cell invasion capacity decreased in both of the cells transfected with miR-770-5p mimic compared to scrambled control-transfected cells (n = 2, **p<0.0001).</p
Involvement of miR-770-5p in trastuzumab response in HER2 positive breast cancer cells
No full textmiRNAs may play effective roles in breast cancer so modulating their expression levels could have therapeutic benefits. Recent studies have found the combination of miRNA-based therapeutics with conventional drugs as promising. This study aimed to find drug-responsive miRNAs, and explore their anticancer activities in HER2+ breast cancer cells and regulatory role in the trastuzumab response. qRT-PCR-array analysis was performed with effective concentrations of tamoxifen and trastuzumab treated BT-474, SK-BR-3 and MCF-7 cells. Motility and invasion analyses were performed with wound healing and xCELLigence impedance-based assays respectively. Viability of cells following mimic transfection and drug treatment was assessed by WST-1 assay. Western blot analysis was used to assess miR-770-5p regulation of proteins and their phosphorylated forms. The clinical relevance of miR-770-5p was examined by TCGA data analysis. The qRT-PCR-array results indicated that miR-770-5p was responsive in a drug and cell line independent manner. Overexpression of miR-770-5p inhibited the motility and cell invasion through regulation of AKT and ERK proteins. Additionally, miR-770-5p potentiated the effectiveness of trastuzumab. Thus, regulating the expression level of miR-770-5p in combination with trastuzumab treatment may simultaneously inhibit the downstream elements of PI3K and MAPK signalling, thereby blocking the proliferation, motility and invasion capacities of HER2+ breast cancer cells.</div
miR-770-5p targeted total ERK in BT-474 and SK-BR-3 cells.
No full textProtein expression of total AKT and ERK were detected in BT-474 and SK-BR-3 cells transfected with miR-770-5p mimics and scrambled control (scr). ERK expression decreased in BT-474 cells after mimic transfection (n = 4, *p<0.01). Both the ERK expression and the AKT expression, decreased significantly only in SK-BR-3 cells (n = 2, **p<0.05).</p
Resolución UNRN N° 770/2009. Aprobar la designación como profesor adjunto.
No full textFil: Universidad Nacional de Río Negro (U). Universidad Nacional de Río Negro. Río Negro, ArgentinaResolución UNRN N° 770/2009. Aprobar la designación como profesor adjunto.fals
Search for the radiative decay D s + → γρ 770 +
No full textAbstract Using 7.33 fb −1 of e + e − collision data samples collected with the BESIII detector at center-of-mass energies between 4.128 and 4.226 GeV, we search for the radiative decay D s + → γρ 770 + for the first time. A hint of D s + → γρ 770 + is observed with a statistical significance of 2.5σ. The branching fraction of D s + → γρ 770 + is measured to be (2.2 ± 0.9stat. ± 0.2syst. ) × 10 −4, corresponding to an upper limit of 6.1 × 10 −4 at the 90% confidence level
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