1,724,106 research outputs found
Multiple linear regression analysis of relative expression of miRNA-6767-5p with reproductive and metabolic variables.
No full textMultiple linear regression analysis of relative expression of miRNA-6767-5p with reproductive and metabolic variables.</p
Correlation analysis between the relative expression of miRNA-6767-5p and reproductive/metabolic variables in total subjects.
No full textCorrelation analysis between the relative expression of miRNA-6767-5p and reproductive/metabolic variables in total subjects.</p
محضر رقم 6767 اعمال من اللجنة الاستشارية للدراسات الاكاديمية
No full textمحضر رقم 6767 اعمال من اللجنة الاستشارية للدراسات الاكاديميةمحضر رقم 6767 اعمال من اللجنة الاستشارية للدراسات الاكاديمي
6767 is a MEG-2 Family member.
No full text<p>Panel A: Alignment of MEG-2 family members. Proteins include an ESP-15 (CAZ37453.2) and 6767, a MEG-2 family member that is enriched in females from single sex infections. Alignments were generated using ClustalW and Boxshade. Panel B: Exon map of 6767 on <i>S. mansoni</i> genome fragment SC_0319. Exons are designated a-l, sizes of exons are listed below exons.</p
Effect of miR-6767-5p on breast cancer cell phenotype and its regulatory mechanism
No full textAbstract Objective To investigate the role and mechanism of miR-6767-5p in breast cancer (BC). Methods: We explored the effects of miR-6767-5p on the proliferation, migration, and invasion of BC cells in vitro and in vivo through CCK-8, EdU, Transwell, and subcutaneous tumorigenesis experiments in nude mice and a tail vein lung metastasis model. Cysteine-rich intestinal protein 2 (CRIP2) was validated as a target gene of miR-6767-5p through dual-luciferase reporter assays, quantitative polymerase chain reaction (qPCR), and western blot (WB) analysis. WB was conducted to investigate the impact of miR-6767-5p on the NF-κB signaling pathway and its association with the epithelial‒mesenchymal transition (EMT). Coimmunoprecipitation, chromatin immunoprecipitation, qPCR and WB were used to verify the possible relationships among SP1, c-jun and miR-6767-5p. The relationships between miR-6767-5p and the stage and prognosis of breast cancer were investigated by in situ hybridization. Results: (1) Knockdown of miR-6767-5p inhibits the proliferation, migration, and invasion of BC cell in vivo and in vitro. (2) miR-6767-5p targets CRIP2. (3) miR-6767-5p activates NF-κB and the EMT by inhibiting CRIP2. (4) miR-6767-5p can be upregulated by SP1. (5) Mitogen-activated protein kinase kinase 4 (MAP2K4) induces miR-6767-5p expression through the PI3K/AKT/c-jun/SP1 axis. (6) High expression of miR-6767-5p is associated with a poor prognosis for patients with breast cancer. Conclusions: (1) miR-6767-5p is highly expressed in BC cells, stimulating their proliferation, migration, and invasion both in vivo and in vitro. (2) miR-6767-5p activates the NF-κB signaling pathway and the EMT by specifically inhibiting CRIP2. (3) The expression of miR-6767-5p is regulated by MAP2K4 through the upregulation of c-jun. (4) The expression of miR-6767-5p is positively correlated with the stage of breast cancer and a poor prognosis for patients
The Role of Serum MicroRNA-6767-5pas a Biomarker for the Diagnosis of Polycystic Ovary Syndrome
No full textBackground Polycystic ovary syndrome (PCOS) is a heterogeneous disorder, and the underlying molecular mechanisms are not clear. To date, few studies have been conducted on the altered expression of serum microRNAs (miRNAs) in women with PCOS. The present study was performed to examine the role of the serum miRNA as a biomarker for the diagnosis of PCOS and its relationship with metabolic and reproductive traits. Methods A cross-sectional comparison was made in 21 women with PCOS and age-and body mass index (BMI)-matched 21 healthy women in an academic center laboratory between December 2008 and October 2010. We selected miRNAs that were more than 1.5-fold up-regulated or less than 0.67-fold down-regulated in women with PCOS compared with controls using the SurePrint G3 Human miRNA Microarray. Subsequently, we validated the relative expression of the miRNAs using TaqMan quantitative real-time polymerase chain reaction (RT-qPCR) assays. Results Serum miRNA-4522, miRNA-324-3p, and miRNA-6767-5p were down-regulated in women with PCOS compared with controls in the microarray analysis. Among these miRNAs, serum miRNA-6767-5p was validated (fold change in women with PCOS/controls = 0.39, P-value&amp;lt;0.05) by RT-qPCR. The miRNA-6767-5p was negatively associated with fasting glucose (beta = -0.370) and positively associated with the number of menses per year (beta = 0.383) after adjustment for age and BMI (Ps&amp;lt;0.05). Genes targeted by miRNA-6767-5p were involved in the cell cycle and the immune system. Conclusions Serum miRNA-6767-5p may be a novel candidate as a molecular biomarker in the diagnosis of PCOS and may participate in the development of the metabolic and reproductive traits of PCOS
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
The Role of Serum MicroRNA-6767-5p as a Biomarker for the Diagnosis of Polycystic Ovary Syndrome.
No full textPolycystic ovary syndrome (PCOS) is a heterogeneous disorder, and the underlying molecular mechanisms are not clear. To date, few studies have been conducted on the altered expression of serum microRNAs (miRNAs) in women with PCOS. The present study was performed to examine the role of the serum miRNA as a biomarker for the diagnosis of PCOS and its relationship with metabolic and reproductive traits.A cross-sectional comparison was made in 21 women with PCOS and age- and body mass index (BMI)- matched 21 healthy women in an academic center laboratory between December 2008 and October 2010. We selected miRNAs that were more than 1.5-fold up-regulated or less than 0.67-fold down-regulated in women with PCOS compared with controls using the SurePrint G3 Human miRNA Microarray. Subsequently, we validated the relative expression of the miRNAs using TaqMan quantitative real-time polymerase chain reaction (RT-qPCR) assays.Serum miRNA-4522, miRNA-324-3p, and miRNA-6767-5p were down-regulated in women with PCOS compared with controls in the microarray analysis. Among these miRNAs, serum miRNA-6767-5p was validated (fold change in women with PCOS/controls = 0.39, P-value<0.05) by RT-qPCR. The miRNA-6767-5p was negatively associated with fasting glucose (β = -0.370) and positively associated with the number of menses per year (β = 0.383) after adjustment for age and BMI (Ps<0.05). Genes targeted by miRNA-6767-5p were involved in the cell cycle and the immune system.Serum miRNA-6767-5p may be a novel candidate as a molecular biomarker in the diagnosis of PCOS and may participate in the development of the metabolic and reproductive traits of PCOS
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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