1,726,639 research outputs found
Course Syllabus FSAD 6750-Sp10
No full textFSAD 6750, Aesthetics and Meaning in World Dress, Course Syllabus by Instructor Charlotte Jirousek, Spring 201
HD470/6750 Course Syllabus - Fall 2017
No full textSyllabus for HD4750/6750 Quantative Methods 1 taught by Felix Thoemmes in term Fall 2017
Environmental Technologies at 6750 Bryan Dairy Rd in Largo, D
No full textThe building for Environmental Technologies, a corporation located at 6750 Bryan Dairy Road in Largo, Florida.https://digitalcommons.usf.edu/gandy_street/6104/thumbnail.jp
6750 sayılı kanun kapsamında işletme rehni
Full text linkBu çalışma, 6750 sayılı Kanun kapsamında işletmelerin bir bütün olarak veya münferit varlıklarının rehnini konu almaktadır. Çalışmamızın ilk bölümünde, işletme kavramı ve işletmelerin finansmana erişim sorunsalı üzerinde durulmuştur. Bu anlamda işletmelerin finansmana erişiminin kolaylaştırılması prensibi ve küresel düzeyde bu yönde gerçekleştirilen reform çalışmaları ve örnekleri değerlendirilmiştir. İkinci bölümde, genel olarak rehin ve rehne ilişkin temel düzenleme biçimi ele alınmıştır. Arkasından, rehin hukukunun genel ilkeleri ve bunların işletme rehnindeki yansımaları hususu irdelenmiştir. Nihayet bu bölümde, işletme rehninin ülkemizdeki tarihi gelimişine de yer verilmiştir. Çalışmamızın üçüncü bölümünde, işletme rehni sözleşmelerinin şekli, rehin hakkının kurulması ve rehnin tarafları hususlarına değinilmiştir. Dördüncü bölümünde, 6750 sayılı Kanun çerçevesinde işletmelerin bir bütün olarak rehni, rehnin kapsamı, koşulları ve sonuçları ile işletmelerin münferit olarak varlıklarının rehnine ilişkin tespit ve değerlendirmelerde bulunulmuştur. Çalışmamızın beşinci ve son bölümünde, işletme rehni, işletme rehninin hüküm ve sonuçları ile bu anlamda özellikle tarafların hak ve yükümlülükleri incelenmiştir
Environmental Technologies at 6750 Bryan Dairy Rd in Largo, A
No full textThe building for Environmental Technologies, a corporation located at 6750 Bryan Dairy Road in Largo, Florida.https://digitalcommons.usf.edu/gandy_street/6101/thumbnail.jp
Environmental Technologies at 6750 Bryan Dairy Rd in Largo, B
No full textThe building for Environmental Technologies, a corporation located at 6750 Bryan Dairy Road in Largo, Florida.https://digitalcommons.usf.edu/gandy_street/6102/thumbnail.jp
Environmental Technologies at 6750 Bryan Dairy Rd in Largo, C
No full textThe building for Environmental Technologies, a corporation located at 6750 Bryan Dairy Road in Largo, Florida.https://digitalcommons.usf.edu/gandy_street/6103/thumbnail.jp
Generation of cell lines to complement Adenovirus vectors using recombination-mediated cassette exchange
Full text linkBackground
Adenovirus serotype 5 (Ad5) has many favourable characteristics for development as a gene therapy vector. However, the utility of current Ad5 vectors is limited by transient transgene expression, toxicity and immunogenicity. The most promising form of vector is the high capacity type, which is deleted for all viral genes. However, these vectors can only be produced to relatively low titres and with the aid of helper virus. Therefore a continuing challenge is the generation of more effective Ad5 vectors that can still be grown to high titres. Our approach is to generate complementing cell lines to support the growth of Ad5 vectors with novel late gene deficiencies.
Results
We have used LoxP/Cre recombination mediated cassette exchange (RMCE) to generate cell lines expressing Ad5 proteins encoded by the L4 region of the genome, the products of which play a pivotal role in the expression of Ad5 structural proteins. A panel of LoxP parent 293 cell lines was generated, each containing a GFP expression cassette under the control of a tetracycline-regulated promoter inserted at a random genome location; the cassette also contained a LoxP site between the promoter and GFP sequence. Clones displayed a variety of patterns of regulation, stability and level of GFP expression. Clone A1 was identified as a suitable parent for creation of inducible cell lines because of the tight inducibility and stability of its GFP expression. Using LoxP-targeted, Cre recombinase-mediated insertion of an L4 cassette to displace GFP from the regulated promoter in this parent clone, cell line A1-L4 was generated. This cell line expressed L4 100K, 22K and 33K proteins at levels sufficient to complement L4-33K mutant and L4-deleted viruses.
Conclusions
RMCE provides a method for rapid generation of Ad5 complementing cell lines from a pre-selected parental cell line, chosen for its desirable transgene expression characteristics. Parent cell lines can be selected for high or low gene expression, and for tight regulation, allowing viral protein expression to mirror that found during infection. Cell lines derived from a single parent will allow the growth of different vectors to be assessed without the complication of varying complementing protein expression
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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