1,722,920 research outputs found

    Linked collectors and determiners for: Review of the Helochares (Hydrobaticus) MacLeay of the New World (Coleoptera: Hydrophilidae: Acidocerinae).

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    Natural history specimen data linked to collectors and determiners held within, "Review of the Helochares (Hydrobaticus) MacLeay of the New World (Coleoptera: Hydrophilidae: Acidocerinae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/8e0aac3a-6556-4b96-a67e-fce9f0db0ba9">https://bionomia.net/dataset/8e0aac3a-6556-4b96-a67e-fce9f0db0ba9</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/8e0aac3a-6556-4b96-a67e-fce9f0db0ba9">https://gbif.org/dataset/8e0aac3a-6556-4b96-a67e-fce9f0db0ba9</a>. Formatted as a Frictionless Data package

    SECRETORY EXPRESSION OF THE EXO-INULINASE FROM K. MARXIANUS CBS 6556 IN PICHIA PASTORIS

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    Inulin is linear oligosaccharides [1]. Although inulin can be hydrolyzed to monosaccharides non-enzymatically by acid treatment, inhibitory by-products produced that complicate downstream biological process. Therefore, it is pivotal to establish a cost-effective inulinase (EC 3.2.1.7) production. In this study, we isolated the DNA sequence encoding the mature peptide sequence of the exo-inulinase gene from Kluyveromyces marxianus CBS 6556 and expressed in Pichia pastoris X-33. The purified recombinant enzyme (reINU) gave a specific activity of 13100 U•mg−1, which was about 100-fold higher that reported for the wild type protein isolated from K. marxianus CBS6556 [2, 3]. Ferguson plot analysis showed that secretion expressed reINU was a 169 kDa dimer. Detailed analytical assays showed that the optimal temperature and pH for reINU were 60 oC and pH 4.0, respectively. It was found that hydrolysis activity was about 20% higher in the presence of Mn2+. reINU was stable for over 3 days at room temperature. When employed for hydrolysis of the juice of the fresh tuber (water/tuber, 1:1) of Helianthus tuberosus at 50 oC for 1 h, total reduced sugar reached 65 g•l−1, and 85% of the total sugar of the raw material was released based on ion chromatography analysis. In conclusion, we have developed an overproduction system for inulinase that can now be used as biocatalysts for fructose syrups industry and biomass conversion research

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Screening on the use of Kluyveromyces marxianus CBS 6556 growing cells as enantioselective biocatalyst for ketones reduction

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    Kluyveromyces marxianus CBS 6556 growing cell versatility in the enantioselective reduction of ketone-carbonyl functionality to the corresponding alcohols was exploited. In particular, methyl ketones were reduced to (S)-alcohols with ee up to 96%. Longer chain alkyl ketones afford, in the same experimental condition, (R)-alcohols with ee up to 84%. Interestingly, carbon-carbon double and triple bond of ketones can also be reduced in the presence of Kluyveromyces marxianus CBS 6556 yeast. Endocyclic carbonyl functionality, such as 2-tetralone carbonyl, is also quantitatively reduced to its (S)-alcohol with ee = 76%

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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