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[ART 5850 syllabus]
No full textA syllabus for ART 5850: Topics in Education Teaching at the University of North Texas
Diffuse interstellar bands: Resolved rotational band structure at 5850 angstrom
No full textThe only candidate of what may be a resolved rotational vibrational band in the DIB Survey of Jenniskens & Desert (1994), the tripler of bands at 5844, 5850 and 5852 Angstrom, is examined in detail in order to establish the nature of these DIBs. We find that superposed on the broad lambda 5844 are four weak features, reminiscent of substructure found on top of the broad lambda 5778. The relative band strength of DIBs lambda 5844 and lambda 5850 correlates with the rotational temperature in the J = 0 and J = 1 levels of molecular hydrogen. However, we do not find variations in band shape that are consistent with substructure of a resolved rotational band profile. Instead, the narrow lambda 5850 is found to have a shoulder on the red and blue side of the main band, which can imply that this band itself is an unresolved rovib band with P,Q and R branches
Block Card 5850 Curson Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Dwelling | 5850 Curson Drive (Toledo, Ohio) | Craftsman Style | McLains Hopewell Heights (Toledo, Ohio) | Greenwood Area (Toledo, Ohio) | West Toledo Area (Toledo, Ohio) | Folk House Styl
MSMEG 5850, a stress-induced TetR protein, involved in global transcription regulation in Mycobacterium smegmatis - Supplementary Figure 1
No full textAim: To decipher the role of MSMEG 5850 in the physiology of mycobacteria. Methods: MSMEG 5850 was knocked out and RNA sequencing was performed. MSMEG 5850 protein was purified from the Escherichia coli pET28a system. Electrophoretic mobility shift assay and size exclusion chromatography were used to determine the binding of MSMEG 5850 to its motif and binding stoichiometry. The effect of nutritional stress was monitored. Results: Transcriptome analysis revealed the differential expression of 148 genes in an MSMEG 5850 knockout strain. MSMEG 5850 had control over 50 genes because those genes had a binding motif upstream of their sequence. The electrophoretic mobility shift assay showed MSMEG 5850 bound to its motif as a monomer. MSMEG 5850 was upregulated under nutritional stress and promoted the survival of mycobacteria. Conclusion: The study confirms the role of MSMEG 5850 in global transcriptional regulation.</p
MSMEG 5850, a stress-induced TetR protein, involved in global transcription regulation in Mycobacterium smegmatis - Supplementry tables
No full textAim: To decipher the role of MSMEG 5850 in the physiology of mycobacteria. Methods: MSMEG 5850 was knocked out and RNA sequencing was performed. MSMEG 5850 protein was purified from the Escherichia coli pET28a system. Electrophoretic mobility shift assay and size exclusion chromatography were used to determine the binding of MSMEG 5850 to its motif and binding stoichiometry. The effect of nutritional stress was monitored. Results: Transcriptome analysis revealed the differential expression of 148 genes in an MSMEG 5850 knockout strain. MSMEG 5850 had control over 50 genes because those genes had a binding motif upstream of their sequence. The electrophoretic mobility shift assay showed MSMEG 5850 bound to its motif as a monomer. MSMEG 5850 was upregulated under nutritional stress and promoted the survival of mycobacteria. Conclusion: The study confirms the role of MSMEG 5850 in global transcriptional regulation.</p
Radio and X-ray nebulae associated with PSR J1509-5850
No full textWe have discovered a long radio trail at 843 MHz which is
apparently associated with middle-age pulsar PSR J1509-5850. The radio trail has
a length of ~7 arcmin. In X-rays, Chandra observation of PSR J1509-5850
reveals an associated X-ray trail which extends in the same orientation
as the radio trail. Moreover, two clumpy structures are observed along the radio trail. The larger one is proposed to be the supernova remnant (SNR) candidate MSC 319.9-0.7. Faint X-ray enhancement at the position of the SNR candidate is found in the Chandra data.
MSMEG 5850, a stress-induced TetR protein, involved in global transcription regulation in Mycobacterium smegmatis - Supplementary Figure 2
No full textAim: To decipher the role of MSMEG 5850 in the physiology of mycobacteria. Methods: MSMEG 5850 was knocked out and RNA sequencing was performed. MSMEG 5850 protein was purified from the Escherichia coli pET28a system. Electrophoretic mobility shift assay and size exclusion chromatography were used to determine the binding of MSMEG 5850 to its motif and binding stoichiometry. The effect of nutritional stress was monitored. Results: Transcriptome analysis revealed the differential expression of 148 genes in an MSMEG 5850 knockout strain. MSMEG 5850 had control over 50 genes because those genes had a binding motif upstream of their sequence. The electrophoretic mobility shift assay showed MSMEG 5850 bound to its motif as a monomer. MSMEG 5850 was upregulated under nutritional stress and promoted the survival of mycobacteria. Conclusion: The study confirms the role of MSMEG 5850 in global transcriptional regulation.</p
Abstract 5850: Nrf2-mediated oxidative stress response is altered during acquired resistance to the proteasome inhibitor, oprozomib, in multiple myeloma
No full textAbstract Multiple myeloma (MM) is a hematologic neoplasm characterized by malignant proliferation of plasma cells in the bone marrow. Proteasome inhibitors are widely used in treatment regimens for MM. Although initial responses to PI (e.g., bortezomib, carfilzomib) treatments have been promising, patients often develop resistance and become refractory to disease. Understanding molecular alterations in signaling cascades influenced by proteasome inhibitors and mechanisms underlying acquired resistance is needed. In this study, we have established a clinically relevant oproxomib-resistant subline (KMS28BMONYX) of the MM cell line KMS28BM. The KMS28BMONYX cell line is pan-resistant to PIs with a 10-fold increase in IC50 for oprozomib as compared to the parental line. To identify genes involved in modulating drug resistance, we analyzed gene expression profiles of both parental and resistant cell lines using the Affymetrix GeneChip Human Genome U133 Plus 2.0 array. Ingenuity Pathway Analysis of microarray data comparing the parental and resistant cells revealed an acute dependence on stress response proteins to maintain PI-resistance. Activation of nuclear factor-erythroid 2 (NF-E2)-related factor 2 (Nrf2; gene symbol NFE2L2) coupled with elevated levels of sequestosome 1/p62 (SQSTM1/p62) were prominent features of the KMS28BMONYX cell line. Altered levels of SQSTM1 correlated with resistance to oprozomib in several MM cell lines. Simultaneously, the KMS28BMONYX cell line showed increased expression of MYC and MCL1. Oprozomib treatment stabilized c-Myc expression in the KMS28BMONYX line. The Champion ChiP Transcription Factor Search Portal database DECODE predicted two c-Myc transcription factor binding sites in the SQSTM1 promoter. CHIP-seq data for MYC in MM1s cells also indicates strong binding in the promoter region of SQSTM1. Our data suggest that therapies targeting the SQSTM1/p62-Nrf2 pathway may help overcome proteasome inhibitor resistance in refractory MM patients. Citation Format: Snehal M. Gaikwad, Adriana Zingone, Aleksandra Michalowski, Susana Najera, Anaisa Quintanilla-Artega, Sayeh Gorjifard, John Simmons, Nick Watson, Ola Landgren, Jing Huang, Beverly Mock. Nrf2-mediated oxidative stress response is altered during acquired resistance to the proteasome inhibitor, oprozomib, in multiple myeloma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 5850
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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