1,724,187 research outputs found
Block Card 5447 Fern Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: End Gabled | 5447 Fern Drive (Toledo, Ohio) | Dwelling | Fernwood Place (Toledo, Ohio) | Trilby Area (Toledo, Ohio)) | Folk House Styl
Block Card 5447 Lewis Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: 5447 Lewis Avenue (Toledo, Ohio) | duplexes | Greenwood Park Area (Toledo, Ohio) | Modern Colonial Styl
Eléments orbitaux d'étoiles doubles: ADS 5447 et ADS 8035
No full textNous présentons de nouveaux éléments orbitaux pour les étoiles doubles visuelles ADS 5447 et 8035. Ces éléments ont été obtenus en utilisant les dernières mesures que nous avons effectuées avec le tavelographe PISCO et le télescope Zeiss de 102 cm de Merate (Italie), et celles déjà disponibles dans la littérature
Eléments orbitaux d'étoiles doubles: ADS 5447 et 8035
No full textNous présentons de nouveaux éléments orbitaux pour les étoiles doubles visuelles ADS 5447 et 8035. Ces éléments ont été obtenus en utilisant les dernières mesures que nous avons effectuées avec le tavelographe PISCO et le télescope Zeiss de 102 cm de Merate (Italie), et celles déjà disponibles dans la littérature
Corrections to “Bit-interleaved coded modulation in the wideband regime” [Dec 08 5447-5455]
No full textIn the above titled paper (ibid., vol. 54, no. 12, pp. 5447-5455, Dec. 08), there are three errors that are corrected here
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Abstract 5447: Overexpression of DARPP-32 promotes activation of STAT3 through IGF1R-SRC axis in gastric cancer
No full textAbstract Background: Dopamine and cAMP-regulated phosphoprotein, Mr 32000 (DARPP-32), is a novel cancer gene overexpressed in 2/3 of gastric cancer patients. DARPP-32 promotes cancer cell survival, drug resistance, and invasion. Activation of STAT3, signal transducer and activator of transcription 3, is important for tumorigenesis and drug resistance. Among factors that regulate STAT3, SOCS3 acts as a negative regulator of STAT3 to prevent its uncontrolled oncogenic activation. In this study, we aimed to investigate if DARPP-32 regulates STAT3 and determine the underlying molecular mechanisms. Methods: The mRNA and protein levels were evaluated by quantitative real-time PCR and Western blot analysis. The association between DARPP-32 and STAT3 was evaluated by immunofluorescence. SOCS3 degradation was analyzed by Ubiquitination Assay. The interaction between DARPP-32 and IGF1R was evaluated by co-immunoprecipitation assays. Luciferase reporter and immunohistochemistry stain were used in this study. Results: Overexpression of DARPP-32 in AGS gastric cancer cells increased phosphorylation of STAT3 (Y705) and activation of STAT3 luciferase reporter (P<0.01), and decreased SOCS3 protein expression. DARPP-32 expression increased phosphorylation and ubiquitination of the SOCS3 protein, and subsequently shortened the SOCS3 protein half-life. We provide evidence showing that DARPP-32 promotes activation of IGF1R-SRC axis, critical for phosphorylation and degradation of SOCS3, a negative regulator of STAT3 activity. Proximity ligation and co-immunoprecipitation assays indicated that DARPP-32 and IGF1R co-exist in the same protein complex where DARPP-32 binding to IGF1R promotes its phosphorylation with activation of downstream SRC and STAT3. We obtained double knockouts mice (Darpp32KO/Tff1KO) to determine the histological changes at 3 age groups (3, 6, 9- 12 month). Using the different age groups, we found that the double knockout mouse model have significantly lower incidence of LGD at the age of 3 month, as compared to TFF1 KO (P<0.05) mice. The Darpp32KO/Tff1KO gastric mucosa demonstrated lack of phosphorylation and nuclear localization of STAT3 with significant delay in the onset of tumor lesions. Immunohistochemistry analysis confirmed overexpression of DARPP-32 and nuclear localization of STAT3 in human gastric cancers. Conclusion: The in vitro studies indicate that DARPP-32 plays a critical role in activation of STAT3 signaling through regulation of IGF1R and SRC, leading to SOCS3 degradation. In vivo results suggest that lack of DARPP-32 delays the development of tumors in the TFF1 knockout mouse model but is not sufficient alone to abrogate the full carcinogenesis cascade. These novel findings add to the oncogenic functions of DARPP-32 and highlight its potential role in the pro-inflammatory gastric carcinogenesis cascade. Citation Format: Shoumin Zhu, Mohammed Soutto, Zheng Chen, Wael El-Rifai. Overexpression of DARPP-32 promotes activation of STAT3 through IGF1R-SRC axis in gastric cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 5447
Characteristics of 5447<sup>b</sup> patient visits in the study.
No full texta<p>Presence of <i>P. falciparum</i> parasites was analyzed by thick blood smear or thin blood film.</p>b<p>5447 visits of 3641 individuals were included in the study.</p
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