1,760,561 research outputs found
ILK is the target gene of miR-542-3p.
No full text(A) We first found the binding sites for miR-542-3p in ILK 3 ’UTR via bioinformatics analysis, (B) then ILK 3 ’UTR -Wt and ILK 3 ’UTR -Mut were designed, and the results of dual luciferase report assay revealed that the luciferase activity of the Wt reporter in the ILK-Wt+miR-542-3p mimics subset was significantly reduced compared to 3 other subsets. (C) The effect of transfected miR-542-3p mimic on the expression of the ILK protein was detected by WB. ILK protein was downregulated in miR-542-3p mimics subsets both in SW620 and HCT116. Three subsets were designed and compared to blank or miR-NC, ILK in up-regulated miR-542-3p expressed lower. Black represents pmirGLO-ILK 3 ’UTR -Wt and grey represent pmirGLO-ILK 3 ’UTR -Mut in histograms. The right picture is the quantitative analysis of the left one. Black represents SW620 and grey represents HCT116 in histograms. *P < 0.05 and **P < 0.01.</p
Up-regulating miR-542-3p inhibits the proliferation and invasion of CRC cells.
No full textRelative expression of miR-542-3p mimics in SW620 and HCT116 cell lines of CRC were determined by qPCR, Black represents SW620 and grey represents HCT116 in histograms. (A) Subsets were defined as miR-NC and miR-542-3p mimics. MiR-542-3p mimics did upregulate the expression of miR-542-3p significantly. (B&C) On the initial results, we respectively detected the proliferation of SW620 and HCT116 on miR-NC and miR-542-3p mimics subsets at 0, 24, 48, and 72h via CCK8, and found that SW620 with miR-542-3p mimics proliferated significantly slower than miR-NC subset after 72 hours, while HCT116 with miR-542-3p mimics proliferated significantly slower than miR-NC subset after 48 hours. Blue represents the miR-NC subset, and red represents miR-542-3p mimics. (D) Edu assay showed miR-542-3p mimics significantly inhibited CRC from proliferating. Histograms of Edu positive cells in miR-NC and miR-542-3p mimics subsets are the quantitative analysis of pictures. (E&F) Transwell was used for the invasion and migration of SW620 and HCT116 cell lines. Both invasion and migration were inhibited in miR-542-3p mimics subsets. The upper picture is about migration, and the below one is about invasion. The histograms are the quantitative analysis of the left pictures. Scale bar = 50 μm. *P < 0.05 and **P < 0.01.</p
LncRNA <i>CASC9</i> negatively regulates miR-542-3p by molecular sponge adsorption.
No full text(A) the binding sites for miR-542-3p in lncRNA CASC9 were prompted by bioinformatics analysis. (B) Dual-luciferase reporter gene assay showed that that the luciferase activity of the Wt reporter in the lncRNA CASC9-Wt+miR-542-3p mimics subset was significantly decreased compared to 3 other subsets. Black represents pmirGLO-CASC9-Wt and grey represents pmirGLO-CASC9-Mut in histograms. (C&D) In RNA-IP assay, the fold enrichment in the Ago2 subset was significantly higher than the IgG subset, which further confirmed lncRNA CASC9 could combine miR-542-3p by predicted binding sites both in SW620 and HCT116. Mouse IgG and human Ago2 were used in lncRNA CASC9 and miR-542-3p subsets of CRC cell lines. (E)The relative expression of miR-542-3p of both NC and sh-CASC9-2 in SW620 and HCT116 were detected via qPCR, and downregulating lncRNA CASC9 upregulated miR-542-3p. Black represents SW620 and grey represents HCT116 in histograms. *P < 0.05 and **P < 0.01.</p
Overexpression of ILK significantly restores the proliferation and invasion of CRC cells with downregulated lncRNA <i>CASC9</i> or upregulated miR-542-3p.
No full text5 groups were designed, they are blank, sh-CASC9-2, sh-CASC9-2+pcDNA2.1-ILK, miR-542-3p mimics, and miR-542-3p mimics+pcDNA3. Black represents SW620 and grey represents HCT116 in histograms. (A) Transwell was used for invasion on 5 subsets of SW620 and HCT116 cell lines. Both down-regulating lncRNA CASC9 and up-regulating miR-542-3p could inhibit the invasion, while overexpression of ILK could significantly restore part of proliferation which is inhibited by downregulated lncRNA CASC9 and upregulated miR-542-3p, not only in SW620 but also in HCT116. (B) Edu assay was determined by comparing the proliferation among 5 groups. Compared to down-regulating lncRNA CASC9 and up-regulating miR-542-3p, overexpression of ILK significantly improved the proliferation inhibited by downregulated lncRNA CASC9 and upregulated miR-542-3p. Histograms are the quantitative analysis of pictures. Scale bar = 50 μm. *P < 0.05 and **P < 0.01.</p
El Tlacuache Núm. 542 (2012). 542 Año 13 (2012) noviembre. El Tlacuache
No full textLas penas con pan son buenas por Adrián Fuentes Aguirre, Arturo Monteros Guijón. -Cerámica para nuestros difuntos por Marcos Garma Nopaltitla, Enrique Méndez Torres
miR-542-3p suppresses colorectal cancer progression through targeting survivin
No full textBackground: miR-542-3p has been reported to be a tumor suppressor in several tumor types, while its role in colorectal cancer (CRC) has not been fully understood. This study aimed to investigate the expression of miR-542-3p and its potential role in human CRC. Methods: Real-time PCR was used to detect the expression of miR-542-3p in tissues and plasma of CRC patients. The impact of miR-542-3p on the aggressive phenotypes of CRC cells were evaluated by in vitro functional assays. Luciferase activity assay was conducted to confirm the direct binding of miR-542-3p to survivin. Results: miR-542-3p was decreased in CRC cell lines that derived from metastatic sites. Among the 65 CRC patients enrolled in this study, 63.08% (41/65) had a decreased miR-542-3p expression in cancerous tissues. miR-542-3p expression was associated with lymphovascular invasion (P=0.008), distant metastasis (P=0.006), tumor stage (P=0.034) and patients' survival (P=0.027). A decreased expression of miR-542-3p in plasma was detected in stage IV patients. In vitro and in vivo experiments showed that miR-542-3p could inhibit the aggressive phenotypes of CRC cells. Finally, survivin was identified as a direct target of miR-542-3p in CRC. Conclusions: Decreased expression of miR-542-3p in CRC patients was associated with unfavourable clinicopathological features of the patients. miR-542-3p inhibits the aggressive phenotypes of CRC cell lines. Survivin is a direct target of miR-542-3p in CRC.National Natural Science [81372290, 81572379, 81572383]SCI(E)[email protected]; [email protected]
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Santa Fe (ATSF) 542
No full textA photograph print showing Santa Fe (ATSF) 542, 4-4-2 (Class 542), Shopton, IA. Back of photo lists loco's specifications
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