1,725,495 research outputs found
Recommended from our members
Abstract 4820: The disassembly of amyloid-bodies
No full textAbstract The ability of cells to adapt to a wide variety of stress conditions plays a critical role in various physiological and pathological settings, including development, cancer and neurological disorders. We recently reported the discovery of stress-induced low complexity noncoding RNA derived from stimuli-specific loci of the ribosomal intergenic spacer (rIGSRNA); an enigmatic region of the human genome historically dismissed as “junk” DNA. We showed that low complexity rIGSRNA activate a physiological amyloidogenic program that converts the nucleolus into Amyloid-bodies: a molecular prison of immobilized proteins in an amyloid-like state. This conserved post-translational regulatory pathway enables cells to rapidly and reversibly store an array of endogenous proteins in Amyloid-bodies and enter a dormant-like phenotype in response to severe environmental insults. While many membrane-less compartments have been described as liquid-like (e.g., stress granules, P-bodies, germ cell granules), our discovery of Amyloid-bodies provided evidence of an amyloidogenic process that can physiologically transition biological matter to a solid-like state. The ability of mammalian cells to efficiently dissemble Amyloid-bodies raises a fundamental question: Are mammalian cell disaggregases involved in Amyloid-body disassembly? Here, we show that Amyloid-bodies undergo a solid-to-liquid-phase transition to release sequestered proteins and restore nucleolar functions. An RNAi screened identified key Heat Shock Proteins (Hsps) that remodel Amyloid-bodies back to the liquid nucleoli on stress termination. The composition of mammalian disaggregases differs considerably from non-metazoans and those involved in disassembly of pathological amyloids in vitro. Activation of mammalian disaggregases and Amyloid-body disassembly is dependent on ATP concentration. Conceptually, this work identifies metazoan disaggregases challenging the widely accepted paradigm that the amyloid state is irreversible in mammalian cells. The data also provides alternative insights into pathogenic amyloids by examining their disassembly in cellular systems. Citation Format: Chloe Kirk, Michael Bokros, Alex Grunfeld, Stephen Lee. The disassembly of amyloid-bodies. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 4820
Block Card 4820 Elm Place
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 4820 Elm Place (Toledo, Ohio) | Dwelling | Deveaux Area (Toledo, Ohio
Block Card 4820 Talmadge Road
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch Style | 4820 Talmadge Road (Toledo, Ohio) | Dwelling | Estero Place (Toledo, Ohio) | Trilby Area (Toledo, Ohio)
Block Card 4820 Oakridge Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | Dwelling | 4820 Oakridge Drive (Toledo, Ohio) | Secor Woods Addition (Toledo, Ohio) | Trilby Area (Toledo, Ohio)) | West Toledo Area (Toledo, Ohio
Linked collectors and determiners for: Hidden gems in museum cabinets: new species and new distributional records of Scolytodes (Coleoptera: Scolytinae).
No full textNatural history specimen data linked to collectors and determiners held within, "Hidden gems in museum cabinets: new species and new distributional records of Scolytodes (Coleoptera: Scolytinae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/4cc8e6da-6caa-4820-8400-9cc2db63920a">https://bionomia.net/dataset/4cc8e6da-6caa-4820-8400-9cc2db63920a</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/4cc8e6da-6caa-4820-8400-9cc2db63920a">https://gbif.org/dataset/4cc8e6da-6caa-4820-8400-9cc2db63920a</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: A new species of Elaphoglossum sect. Lepidoglossa (Dryopteridaceae) from Bolivia.
No full textNatural history specimen data linked to collectors and determiners held within, "A new species of Elaphoglossum sect. Lepidoglossa (Dryopteridaceae) from Bolivia". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/978b6d1a-2dfd-4820-8e28-5d0def7ba731">https://bionomia.net/dataset/978b6d1a-2dfd-4820-8e28-5d0def7ba731</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/978b6d1a-2dfd-4820-8e28-5d0def7ba731">https://gbif.org/dataset/978b6d1a-2dfd-4820-8e28-5d0def7ba731</a>. Formatted as a Frictionless Data package
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Balaenoptera physalus (fin whale) - UMA-4820
No full textBalaenoptera physalus (fin whale) - UMA-4820 - female 16.8 m - Pelvic location - UMass Amherst. 55 foot (660 inches), female stranded alive at Sunken Meadow Beach, Eastham, Barnstable Co., MA. Euthanized and towed to Jeremy Point, Cape Cod National Seashore, Wellfleet. Anterior 2 feet of left mandible broken off, coronoid processes (where the masseter muscle attaches) broken on each mandible, tip of left maxilla broken off, crack across left maxilla & left scapula with recent granulation of healing bone at all of these sites. The extent of these injuries suggested ship strike. Skull, mandibles, both flippers including scapulas, both pelvic bones, sternum and right first rib salvaged on 8 August for the Univ. of MA, Amherst
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
- …
