1,772,795 research outputs found
Structure, Organization, and Expression of the lct Gene for Lacticin 481, a Novel Lantibiotic Produced by Lactococcus lactis
No full textThe structural gene for the lactococcal lantibiotic lacticin 481 (lct) has been identified and cloned using a degenerated 20-mer DNA oligonucleotide based on the amino-terminal 7 amino acid residues of the purified protein. The transcription of the lct gene was analyzed, and its promoter was mapped. DNA sequence analysis of the lct gene revealed an open reading frame encoding a peptide of 51 amino acids. Comparison of its deduced amino acid sequence with the amino-terminal sequence and the amino acid composition of lacticin 481 indicates that the 61-residue peptide is prelacticin 481, containing a 27-residue carboxyl-terminal propeptide and a 24-residue amino-terminal leader peptide which lacks the properties of a typical signal sequence and which is significantly different from the leaders of other lantibiotics. The predicted amino acid sequence of prolacticin 481 contains 3 cysteines, 2 serines, and 2 threonines which were not detectable in amino acid analyses of mature lacticin 481. Based on these results and on characterization by two-dimensional NMR techniques, a structural model is proposed in which 2 cysteine residues are involved in lanthionine and one in β-methyllanthionine formation, and a 4th threonine residue is dehydrated. This model predicts a molecular mass for lacticin 481 of 2,901, which is in excellent agreement with that obtained from mass spectrometry.
Participant 481 Community Conversations Interview
No full textIn this interview, Participant 481 explains that COVID-19 has changed their life in many ways as a healthcare worker. They share that their schedule increased as work became "all hands on deck," and that while this was beneficial financially, it was a source of mental and emotional exhaustion. The participant shares that witnessing the death toll of the pandemic left them feeling less strong, and expresses that they have felt survivor's guilt. They discuss their disappointments in the pandemic response and perceptions on communities that have not equally benefited, particularly with the development of the COVID-19 vaccines.New Jersey Department of Healt
Resolución UNRN N° 481/2009. Designar docente interina.
No full textFil: Universidad Nacional de Río Negro (U). Universidad Nacional de Río Negro. Río Negro, ArgentinaResolución UNRN N° 481/2009. Designar docente interina.fals
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
El Tlacuache Núm. 481 (2011). 481 Año 11 (2011) agosto. El Tlacuache
No full textAlejandro Salinas, acercamientos a la vida de un museógrafo por Eduardo Corona Martínez. -Rastros de un camino interrumpido anticipadamente por Hortensia de Vega Nova. -Donde quiera que estes por Rafael Gutiérrez. -Alejandro Salinas Museógrafo fundador del Centro INAH Morelos Guerrero y entrañable compañero por Miguel Morayta. -Obra museográfica destacada. -Exitosa la participación de los niños en los cursos de verano delINAH Morelos
Band 1, 481 (Sammlung Friedlaender)
No full textBAND 1, 481 (SAMMLUNG FRIEDLAENDER)
Band 1, 481 (Sammlung Friedlaender) (Public Domain) (1
Band 2, 481 (Sammlung Friedlaender)
No full textBAND 2, 481 (SAMMLUNG FRIEDLAENDER)
Band 2, 481 (Sammlung Friedlaender) (Public Domain) (1
Band 3, 481 (Sammlung Friedlaender)
No full textBAND 3, 481 (SAMMLUNG FRIEDLAENDER)
Band 3, 481 (Sammlung Friedlaender) (Public Domain) (1
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