1,725,145 research outputs found
Gas gangrene in a cat with aortic embolism
No full textRE: 20 ref.; SC: ZA; CA; VE; 0ISource type: Electronic(1) http://upei-resolver.asin-risa.ca?sid=SP:CABI&id=pmid:&id=&issn=0022-4510&isbn=&volume=23&issue=6&spage=359&pages=359-363&date=1982&title=Journal%20of%20Small%20Animal%20Practice&atitle=Gas%20gangrene%20in%20a%20cat%20with%20aortic%20embolism.&aulast=Schiffer&pid=%3Cauthor%3ESchiffer%2c%20S%20P%3bMiller%2c%20L%20M%3C%2Fauthor%3E%3CAN%3E19822206540%3C%2FAN%3E%3CDT%3EJournal%20article%3C%2FDT%3
Necrotizing pulmonary arteritis in a dog with patent ductus arteriosus
No full textRE: 7 ref.; SC: ZA; CA; VE; 0ISource type: Electronic(1) http://upei-resolver.asin-risa.ca?sid=SP:CABI&id=pmid:&id=&issn=0022-4510&isbn=&volume=22&issue=9&spage=603&pages=603-608&date=1981&title=Journal%20of%20Small%20Animal%20Practice&atitle=Necrotizing%20pulmonary%20arteritis%20in%20a%20dog%20with%20patent%20ductus%20arteriosus.&aulast=Turk&pid=%3Cauthor%3ETurk%2c%20J%20R%3bMiller%2c%20L%20M%3bMiller%2c%20J%20B%3bSande%2c%20R%20D%3C%2Fauthor%3E%3CAN%3E19812293235%3C%2FAN%3E%3CDT%3EJournal%20article%3C%2FDT%3
Rim fragment of a shallow Plain-White bowl (125693/4510).
No full textRim fragment of a shallow Plain-White bowl (125693/4510).</p
Block Card 4510 East Way
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Dwelling (Toledo, Ohio) | Ranch Style | Homeville Subdivision (Toledo, Ohio) | Willys Park area (Toledo, Ohio) | West Toledo (Toledo, Ohio) | 4510 East Way (Toledo, Ohio
Block Card 4510 Monac Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 4510 Monac Drive (Toledo, Ohio) | Dwelling | Greenbush Knolls Addition (Toledo, Ohio) | Trilby Area (Toledo, Ohio)
Rim fragment of a shallow Plain-White bowl (125697/4510).
No full textRim fragment of a shallow Plain-White bowl (125697/4510).</p
MiR-4510 suppresses hepatocellular carcinoma development through RAF1 targeting and MAPK/ERK signaling inhibition
No full textLe profil d'expression aberrant des micro(mi)ARN est une caractéristique typique de nombreux cancers, dont le carcinome hépatocellulaire (CHC), une tumeur hépatique maligne primaire qui se classe seconde dans le monde en termes de mortalité par cancer. Notre équipe a récemment montré la baisse d’expression de miR-4510 dans des échantillons de patients atteints de CHC et son activité « suppresseur de tumeur ». L'analyse de données protéomiques recueillies à partir de cellules Huh7 transfectées par miR-4510 a révélé une diminution importante de plusieurs oncogènes, dont la sérine / thréonine protéine kinase RAF1. J’ai également découvert que le taux de protéine RAF1 était significativement surexprimé chez les patients atteints de CHC. Le rôle de RAF1 et de miR-4510 dans le CHC étant mal compris, j’ai étudié la fonction du couple RAF1/miR-4510 dans la tumorigenèse du foie. Mes analyses ont montré que miR-4510 régule négativement les taux de protéine RAF1 et d'ARNm. Une analyse par le système de double fluorescence-FunREG a révélé que miR-4510 interagit directement avec la région 3’ non-traduite de l’ARN de RAF1 via un site unique. La déplétion de RAF1 dans deux lignées tumorales de CHC par miR-4510 ou ARN interférant désactive leur caractère tumorigène in vitro et in vivo. Collectivement, mes données suggèrent que miR-4510 participe à la carcinogenèse du foie via son action directe sur RAF1 et la régulation de la voie MAPK/ERK. En conclusion, mon étude soutient l’hypothèse selon laquelle un traitement à base de miR-4510 pourrait être efficace pour traiter les patients atteints de CHC de type avancé ou réfractaire à la chimiothérapie.Aberrant micro(mi)RNA expression signature is a hallmark of many cancers including hepatocellular carcinoma (HCC), a primary malignant liver disease which ranks second in cancer mortality worldwide. Our team previously reported the downregulation of miR-4510 in HCC samples and identified this miRNA as a strong tumor suppressor in liver. Proteomic data analysis collected from Huh7 cells transfected by miR-4510 showed a significant decrease of multiple oncogenes including RAF1 serine/threonine protein kinase. I also found that RAF1 protein level is significantly increased in HCC patients. The role of RAF1 and miR-4510 in HCC being poorly understood, I studied the function of RAF1/miR-4510 pair in tumorigenesis of the liver. My results showed that miR-4510 overexpression significantly decreases both RAF1 protein and mRNA levels and inhibits MAPK/ERK signaling. The dual fluorescence-FunREG assay revealed that miR-4510 directly interacts with RAF1 3’-untranslated region through a unique site. Silencing of RAF1 in two hepatic cell lines by miR-4510 or a specific small interfering RNA suppressed important tumorigenic features (proliferation, migration….) both in vitro and in vivo. Collectively, my data suggest that miR-4510 participates in liver carcinogenesis through RAF1 targeting and MAPK/ERK signaling inactivation. In addition, my study suggests that miR-4510-based therapy may represent a promising strategy to treat patients with advanced or refractory HCC
miR‐4510 blocks hepatocellular carcinoma development through RAF1 targeting and RAS/RAF/MEK/ERK signalling inactivation
No full textInternational audienceBACKGROUND:Therapeutic outcomes using the multikinase inhibitors, sorafenib and regorafenib, remain unsatisfactory for patients with advanced hepatocellular carcinoma (HCC). Thus, new drug modalities are needed. We recently reported the remarkable capacity of miR-4510 to impede the growth of HCC and hepatoblastoma through Glypican-3 (GPC3) targeting and Wnt pathway inactivation.METHODS:To identify new targets of miR-4510, we used a label-free proteomic approach and reported down-regulation of RAF proto-oncogene serine/threonine-protein kinase (RAF1) by miR-4510. Because the tumourigenic role of RAF1 in HCC is controversial, we further studied RAF1:miR-4510 interactions using cellular, molecular as well as functional approaches and a chicken chorioallantoic membrane (CAM) xenograft model.RESULTS:We found an increase in RAF1 protein in 59.3% of HCC patients and a specific up-regulation of its transcript in proliferative tumours. We showed that miR-4510 inactivates the RAS/RAF/MEK/ERK pathway and reduces the expression of downstream targets (ie c-Fos proto-oncogene [FOS]) through RAF1 direct targeting. At a cellular level, miR-4510 inhibited HCC cell proliferation and migration and induced senescence in part by lowering RAF1 messenger RNA (mRNA) and protein expression. Finally, we confirmed the pro-tumoural function of RAF1 protein in HCC cells and its ability to sustain HCC tumour progression in vitro and in vivo.CONCLUSIONS:In this work, we confirm that RAF1 acts as an oncogene in HCC and further demonstrate that miR-4510 acts as a strong tumour suppressor in the liver by targeting many proto-oncogenes, including GPC3 and RAF1, and subsequently controlling key biological and signalling pathways among which Wnt and RAS/RAF/MEK/ERK signals
Linked collectors and determiners for: Types of Thyatirinae (Lepidoptera: Drepanidae) in the collection of the Zoological Institute of the Russian Academy of Sciences.
No full textNatural history specimen data linked to collectors and determiners held within, "Types of Thyatirinae (Lepidoptera: Drepanidae) in the collection of the Zoological Institute of the Russian Academy of Sciences". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/63db7ca1-2866-4510-9ce8-b9ad97f41703">https://bionomia.net/dataset/63db7ca1-2866-4510-9ce8-b9ad97f41703</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/63db7ca1-2866-4510-9ce8-b9ad97f41703">https://gbif.org/dataset/63db7ca1-2866-4510-9ce8-b9ad97f41703</a>. Formatted as a Frictionless Data package
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