1,724,749 research outputs found
Progesterone Hydroxylation with Colletotrichum Lini AS3. 4486
No full textThe biotransformation of progesterone by Colletotrichum lini AS3. 4486 was studied. The formation of the product was monitored by HPLC. Product was purified from broth culture supernatants by silica gel column chromatography and identified as 7β,12β-dihydoxylprogesterone with MS, 1H NMR, 13C NMR and NOE.</jats:p
miR-4486 inhibits colorectal cancer proliferation via targeting MAP2K4 to inhibit the activation of the p38MAPK/JNK signaling
No full textObjective: Since MAP2K4 was reportedly involved in colorectal cancer development and the p38MAPK/JNK signaling transcription, this study aimed to investigate the mechanism by which the microRNA (miR)-4486 acts on colorectal cell proliferation. Methods: RT-PCR was conducted to measure the expression levels of the MAP2K4 and miR-4486 in NCM460, SW1116, and HCT116 cells. TargetScanHuman site anticipated that MAP2K4 may be a target of miR-4486. The dual-luciferase reporter assay confirmed their relationship. After plasmids of miR-4486 mimic and si-MAP2K4 transfection, MAP2K4 was quantified again, The CCK-8 assay was carried out to assess cell proliferation, while Scratch and Transwell assays were used to evaluate cell migration and invasion. Finally, miR-4486 mimic and SB203580 were applied in HCT116 and SW1116 cells separately or in combination. CCK-8, Scratch and Transwell assay were performed again. In addition, the proteins including c-capase3, Bax, Bcl2, MAP2K4, and the p38MAPK/JNK signaling-related proteins expression levels were quantified by Western blot (WB). Results: Compared with the NCM460 cells, the expression level of MAP2K4 was elevated, while the expression level of miR-4486 was reduced in SW1116 and HCT116 cells. The results showed that elevated levels of miR-4486 suppressed cell proliferation, migration, and invasion in colorectal cells by downregulating MAP2K4 expression. miR-4486 mimic showed similar effects to SB203580, which promoted colorectal cell apoptosis and inhibited the p38 MAPK/JNK signaling transcription. Conclusion: miR-4486 may target MAP2K4 to inhibit colorectal cell proliferation by inhibiting the activation of the p38/JNK signaling pathway
Linked collectors and determiners for: Arizona State University Arthropod Collection.
No full textNatural history specimen data linked to collectors and determiners held within, "Arizona State University Arthropod Collection". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/d4ce98b8-ae7a-4486-a384-9592c3c8f596">https://bionomia.net/dataset/d4ce98b8-ae7a-4486-a384-9592c3c8f596</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/d4ce98b8-ae7a-4486-a384-9592c3c8f596">https://gbif.org/dataset/d4ce98b8-ae7a-4486-a384-9592c3c8f596</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: A review of the spider genus Porrhomma (Araneae, Linyphiidae).
No full textNatural history specimen data linked to collectors and determiners held within, "A review of the spider genus Porrhomma (Araneae, Linyphiidae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/ac892ad5-037a-4486-9fdb-3082b98cbf49">https://bionomia.net/dataset/ac892ad5-037a-4486-9fdb-3082b98cbf49</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/ac892ad5-037a-4486-9fdb-3082b98cbf49">https://gbif.org/dataset/ac892ad5-037a-4486-9fdb-3082b98cbf49</a>. Formatted as a Frictionless Data package
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Linked collectors and determiners for: Tettigettalna josei (Boulard, 1982) (Hemiptera: Cicadoidea): first record in Spain, with notes on the distribution, genetic variation and behaviour of the species.
No full textNatural history specimen data linked to collectors and determiners held within, "Tettigettalna josei (Boulard, 1982) (Hemiptera: Cicadoidea): first record in Spain, with notes on the distribution, genetic variation and behaviour of the species". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/88ce1fb7-0566-4486-bd63-9c1fbc422b30">https://bionomia.net/dataset/88ce1fb7-0566-4486-bd63-9c1fbc422b30</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/88ce1fb7-0566-4486-bd63-9c1fbc422b30">https://gbif.org/dataset/88ce1fb7-0566-4486-bd63-9c1fbc422b30</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: Tettigettalna josei (Boulard, 1982) (Hemiptera: Cicadoidea): first record in Spain, with notes on the distribution, genetic variation and behaviour of the species.
No full textNatural history specimen data linked to collectors and determiners held within, "Tettigettalna josei (Boulard, 1982) (Hemiptera: Cicadoidea): first record in Spain, with notes on the distribution, genetic variation and behaviour of the species". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/88ce1fb7-0566-4486-bd63-9c1fbc422b30">https://bionomia.net/dataset/88ce1fb7-0566-4486-bd63-9c1fbc422b30</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/88ce1fb7-0566-4486-bd63-9c1fbc422b30">https://gbif.org/dataset/88ce1fb7-0566-4486-bd63-9c1fbc422b30</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: ZMUO Common invertebrate collection.
No full textNatural history specimen data linked to collectors and determiners held within, "ZMUO Common invertebrate collection". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/0ab7fc3f-f956-4486-8f7d-a5009b05efdb">https://bionomia.net/dataset/0ab7fc3f-f956-4486-8f7d-a5009b05efdb</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/0ab7fc3f-f956-4486-8f7d-a5009b05efdb">https://gbif.org/dataset/0ab7fc3f-f956-4486-8f7d-a5009b05efdb</a>. Formatted as a Frictionless Data package
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