1,724,921 research outputs found
miR-4317 suppresses non-small cell lung cancer (NSCLC) by targeting fibroblast growth factor 9 (FGF9) and cyclin D2 (CCND2)
No full textAbstract Background Non-small cell lung cancer (NSCLC) is a leading cause of death worldwide. MicroRNAs (miRNAs) have been indicated as crucial actors in cancer biology. Accumulating evidence suggests that miRNAs can be used as diagnostic and prognostic markers for NSCLC. Methods The purpose of this study was to characterize and identify the novel biomarker miR-4317 and its targets in NSCLC. The expression of miR-4317 was analyzed by in situ hybridization (ISH) and quantitative reverse transcription polymerase chain reaction (qRT-PCR). The effect of miR-4317 on proliferation was evaluated through 3–4,5-dimethylthiazol-2-yl-5-3–carboxymethoxyphenyl-2-4-sulfophenyl-2H-tetrazolium (MTS) and colony formation assays, and cell migration and invasion were evaluated through transwell assays. The expression of target proteins and downstream molecules was analyzed by qRT-PCR and western blot. Dual-luciferase reporter assay was used to assess the target genes of miR4317 in NSCLC cells. Results Our results demonstrated that miR-4317 was downregulated in NSCLC tissues and serum, particularly in lymph node metastasis and advanced clinical stage tissues. Kaplan-Meier survival analysis showed that NSCLC patients with high expression of miR-4317 exhibited better overall survival (OS). Enhanced expression of miR-4317 significantly inhibited proliferation, colony formation, migration and invasion, and hampered cycles of NSCLC cell lines in vitro. Our results suggested that miR-4317 functions by directly targeting fibroblast growth factor 9 (FGF9) and cyclin D2 (CCND2). In concordance with in vitro studies, mouse xenograft, lung, and brain metastatic studies validated that miR-4317 functions as a potent suppressor miRNA of NSCLC in vivo. Systemically delivered agomiR-4317 reduced tumor growth and inhibited FGF9 and CCND2 protein expression. Reintroduction of FGF9 and CCND2 attenuated miR-4317-mediated suppression of migration and invasion in NSCLC. Conclusions Our results indicate that miR-4317 can reduce NSCLC cell growth and metastasis by targeting FGF9 and CCND2. These findings provide new evidence of miR-4317 as a potential non-invasive biomarker and therapeutic target for NSCLC
Linked collectors and determiners for: University of Manitoba (WIN) - Lichen Collection.
No full textNatural history specimen data linked to collectors and determiners held within, "University of Manitoba (WIN) - Lichen Collection". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/cd309a69-421b-4317-a61e-483333be3cb9">https://bionomia.net/dataset/cd309a69-421b-4317-a61e-483333be3cb9</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/cd309a69-421b-4317-a61e-483333be3cb9">https://gbif.org/dataset/cd309a69-421b-4317-a61e-483333be3cb9</a>. Formatted as a Frictionless Data package
Additional file 2: of miR-4317 suppresses non-small cell lung cancer (NSCLC) by targeting fibroblast growth factor 9 (FGF9) and cyclin D2 (CCND2)
No full textFigure S1. miR-4317 overexpression inhibited cell proliferation, colony formation, and migration. a Quantitation of the miR-4317 level after transfection of miR-4317 mimic in A549 cell lines. b The cell growth curve was measured by MTS after transfection of miR-4317 mimic in A549 cell lines, and the OD570 was normalized to the start point (0 h). c Representative images and quantitation of colony formation after transfection of miR-4317 mimic in A549 cell lines. d Representative images and quantitation of the transwell assay after transfection of miR-4317 mimic in A549 cell lines. e miR-4317 induced cell cycle arrest at G1/S phase. Data are presented as the mean values ± SD from triplicate experiments. ♦p < 0.05. (JPG 86 kb
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
ErmineJ significant GO pathways modulated by <i>L</i>. <i>rhamnosus</i> CNCMI–4317 in IECs.
No full text<p>ErmineJ significant GO pathways modulated by <i>L</i>. <i>rhamnosus</i> CNCMI–4317 in IECs.</p
Relaxant and β2-adrenoceptor blocking activities of labetalol, dilevalol, amosulalol and KF-4317 on the rat isolated aorta
No full textAbstract
The KCl-contracted rat aorta is relaxed by labetalol, dilevalol, amosulalol and KF-4317. These relaxations are not reversed by ICI 118,551 at 10−6 M and, therefore, are not due to β-adrenoceptor agonism. At 10−7 M, labetalol, dilevalol, amosulalol and KF-4317 were β2-adrenoceptor antagonists as they inhibited the relaxant responses of rat aorta to procaterol.</jats:p
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Additional file 3: of miR-4317 suppresses non-small cell lung cancer (NSCLC) by targeting fibroblast growth factor 9 (FGF9) and cyclin D2 (CCND2)
No full textFigure S2. Repression of miR-4317 expression significantly promoted cell growth, colony formation, and migration in H157 cells. a Quantitation of the miR-4317 level after transfection of miR-4317 inhibitor in H157 cell lines. b The cell growth curve was measured by MTS after transfection of miR-4317 inhibitor in H157 cell lines, and the OD570 was normalized to the start point (0 h). c Representative images and quantitation of colony formation after transfection of miR-4317 inhibitor in H157 cell lines. d Representative images and quantitation of the transwell assay after transfection of miR-4317 inhibitor in H157 cell lines. e miR-4317 induced cell cycle arrest at G1/S phase. Data are presented as the mean values ± SD from triplicate experiments. ♦p < 0.05. (JPG 590 kb
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
