1,727,700 research outputs found
MicroRNA-4287 alleviates inflammatory response via targeting RIPK1 in osteoarthritis
No full textStudies have confirmed the regulatory effects of microRNAs (miRNAs) in osteoarthritis (OA) progression. MiR-4287 has been identified by a previous study as a downregulated miRNA in chondrocytes treated with IL-1β and TNF-α. However, the function of the underlying mechanism of miR-4287 in OA is elusive. IL-1β-treated chondrocytes were used as OA cell models. RNA expression was accessed using RT-qPCR. Cell Counting Kit-8 (CCK-8) assay was used to determine the chondrocytes' viability and proliferation. The protein levels of inflammation factors (IL-8, IL-6, and TNF-α), matrix metalloproteinases (MMP 1, MMP3, MMP13), and chondrogenic genes (COL2A1, SOX9, and Aggrecan) were detected using western blot analysis. Luciferase reporter assays were performed for interaction exploration. HE staining and Safranin O/Fast Green staining was used to access the pathological changes in OA mouse tissues and cartilage degeneration in OA mouse. MiR-4287 was downregulated in chondrocytes treated with IL-1β and OA mouse models. MiR-4287 overexpression promoted the viability, and proliferation and attenuated the inflammation response and destruction of cartilage in IL-1β-stimulated chondrocytes. Receptor-interacting protein kinase 1 (RIPK1) was a target gene of miR-4287 in chondrocytes. MiR-4287 negatively regulated RIPK1 expression. RIPK1 overexpression was revealed to reverse the miR-4287-mediated effects on proliferation and inflammatory response in IL-1β-stimulated chondrocytes. Moreover, miR-4287 was demonstrated to inhibit the pathological changes, cartilage degeneration and inflammation response in OA mice models. In conclusion, miR-4287 is a critical molecule in OA development, which attenuates inflammatory response in vivo and in vitro by targeting RIPK1
Dynamics of contact line motion during the wetting of rough surfaces and correlation with topographical surface parameters
Get PDFDynamics of contact line motion and wettability is essential in many industrial applications such as liquid coating, lubrication, printing, painting, condensation, etc. However, the wettability of surfaces depends not only on liquid–solid chemical properties but also can be strongly affected by surface roughness. As a practical application of controlled wettability, we can mention the self-cleaning surfaces, protective clothing, microfluidics devices, electro wetting, etc. In this article, we experimentally investigate the spreading of droplets deposited onto rough surfaces. Anisotropic surfaces were prepared by abrasive polishing on the following materials: aluminium alloy AA7064, titanium alloy Ti-6Al-4V, steel AISI 8630, copper alloy UNS C17000, machinable glass ceramic, and poly-methylmethacrylate. Topographical 2D parameters were calculated according to the following standards, defining Geometrical Product Specifications (GPS): ISO 4287, ISO 12085, ISO 13565, ISO 12780, and ISO 12181. The influence of topographical parameters on wettability and spreading phenomenon has been evaluated by statistical covariance analysis. The following parameters have strong influence on fluid spreading on rough surfaces: Rmr is the relative material ratio of the roughness profile, Trc is the microgeometric material ratio, Pmr is the relative material ratio of the raw profile, Kr is the mean slope of the roughness motifs, RONt is the peak to valley roundness deviation, and Psk is the Skewness of the raw profile. The physical meaning of selected parameters is discussed, and Kr (the mean slope of the roughness motifs) is selected as the most important and physically meaningful parameter. It has been found that for all tested materials, fluid spreading shows increasing tendency when mean slope of the roughness motifs (Kr) increases. SCANNING 33: 1–8, 2011. © 2011 Wiley Periodicals, Inc
Fusarium oxysporum f. sp. lycopersici 4287 Genome sequencing and assembly
No full textWe created the reference genome of Fusarium oxysporum f. sp. lycopercisi 4287 for the experimental evolution study
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Linked collectors and determiners for: Revisão do gênero Antiteuchus Dallas (Heteroptera, Pentatomidae, Discocephalinae).
No full textNatural history specimen data linked to collectors and determiners held within, "Revisão do gênero Antiteuchus Dallas (Heteroptera, Pentatomidae, Discocephalinae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/55f509e1-126e-4287-9d18-15a58208e041">https://bionomia.net/dataset/55f509e1-126e-4287-9d18-15a58208e041</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/55f509e1-126e-4287-9d18-15a58208e041">https://gbif.org/dataset/55f509e1-126e-4287-9d18-15a58208e041</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: Entomological slide collection (Luomus).
No full textNatural history specimen data linked to collectors and determiners held within, "Entomological slide collection (Luomus)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/505a4a0e-9430-4287-a2d3-56e3f659ff1c">https://bionomia.net/dataset/505a4a0e-9430-4287-a2d3-56e3f659ff1c</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/505a4a0e-9430-4287-a2d3-56e3f659ff1c">https://gbif.org/dataset/505a4a0e-9430-4287-a2d3-56e3f659ff1c</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: UCSB - University of California Santa Barbara Herbarium.
No full textNatural history specimen data linked to collectors and determiners held within, "UCSB - University of California Santa Barbara Herbarium". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/62cccd4d-eafc-4287-a070-5701aa6ef4a2">https://bionomia.net/dataset/62cccd4d-eafc-4287-a070-5701aa6ef4a2</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/62cccd4d-eafc-4287-a070-5701aa6ef4a2">https://gbif.org/dataset/62cccd4d-eafc-4287-a070-5701aa6ef4a2</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: Herbario Universidad de Caldas - FAUC.
No full textNatural history specimen data linked to collectors and determiners held within, "Herbario Universidad de Caldas - FAUC". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/2f7208c8-b31d-4287-84b4-fc10ab376302">https://bionomia.net/dataset/2f7208c8-b31d-4287-84b4-fc10ab376302</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/2f7208c8-b31d-4287-84b4-fc10ab376302">https://gbif.org/dataset/2f7208c8-b31d-4287-84b4-fc10ab376302</a>. Formatted as a Frictionless Data package
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