1,726,142 research outputs found
Functional characterization of the adhesive and invasive properties of <i>S. aureus</i> 4074, 4074<i>aaa</i>, and 4074<i>aaa</i>(pRCK<i>aaa</i>).
No full text<p>A: Complementation of the 4074<i>aaa</i> mutant. SDS-PAGE (I; 10% separation gel) and zymographic analysis (II) of surface-associated proteins (10 µl) of <i>S. aureus</i> 4074 (lane 1), the 4074<i>aaa</i> mutant (lane 2), and the complemented mutant <i>S. aureus</i> 4074<i>aaa</i> (pRCK<i>aaa</i>) (lane 3). The ∼34 kDa band with lytic activity corresponding to Aaa and its ∼28 kDa putative degradation product were missing from the 4074<i>aaa</i> mutant (lane 2) and present in the wild type (lane 1) and the complemented mutant (lane 3). The arrows indicate Aaa-associated bacteriolytic activity. The sizes of marker proteins (kDa) are indicated on the left. B: Adherence of <i>S. aureus</i> 4074, 4074<i>aaa</i>, and 4074<i>aaa</i> (pRCK<i>aaa</i>) to immobilized Fg, Fn, Vn, and EA.hy 26 cells was assessed by ELISA adherence assays. Binding of the 4074<i>aaa</i> mutant to Vn was significantly decreased in comparison to the wild type. In contrast, binding of the 4074<i>aaa</i> mutant to Fg, Fn, and EA.hy 926 cells was not altered. As a negative control, binding to BSA was assessed. Results are shown as the mean of three independent experiments. Statistical significance is marked by asterisks. C: As a more sensitive method, flow cytometry was applied to determine the binding of FITC-labeled, soluble plasma proteins to <i>S. aureus</i> 4074, 4074<i>aaa</i>, and 4074<i>aaa</i> (pRCK<i>aaa</i>). Again, significantly reduced binding of the 4074<i>aaa</i> mutant was only observed to Vn, whereas its binding to Fg and Fn was unchanged. As a negative control, binding to BSA was assessed. The results are shown as the mean of three independent experiments and statistical significance is marked by asterisks. D: The internalization of strains 4074, 4074<i>aaa</i>, and 4074<i>aaa</i> (pRCK<i>aaa</i>) by EA.hy 926 cells was assessed by flow cytometry and computed in relation to the highly invasive strain <i>S. aureus</i> Cowan 1, which was set to 100% internalization. The internalization of the 4074<i>aaa</i> mutant was in the same range as that of the 4074 wild type and the complemented mutant indicating that Aaa does not play a role in internalization. Results are shown as the mean of three independent experiments.</p
Syllabus - REL/HIST 4074 (Fall 2012)
No full textSyllabus for REL/HIST 4074: Memory & Legend. Taught by Prof. Matthew Gabriele (Dept. of Religion and Culture)
Linked collectors and determiners for: URM - Herbário Pe. Camille Torrand.
No full textNatural history specimen data linked to collectors and determiners held within, "URM - Herbário Pe. Camille Torrand". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/23b714b1-fae6-4074-bdd0-5e17ae3b5f11">https://bionomia.net/dataset/23b714b1-fae6-4074-bdd0-5e17ae3b5f11</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/23b714b1-fae6-4074-bdd0-5e17ae3b5f11">https://gbif.org/dataset/23b714b1-fae6-4074-bdd0-5e17ae3b5f11</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: URM - Herbário Pe. Camille Torrand.
No full textNatural history specimen data linked to collectors and determiners held within, "URM - Herbário Pe. Camille Torrand". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/23b714b1-fae6-4074-bdd0-5e17ae3b5f11">https://bionomia.net/dataset/23b714b1-fae6-4074-bdd0-5e17ae3b5f11</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/23b714b1-fae6-4074-bdd0-5e17ae3b5f11">https://gbif.org/dataset/23b714b1-fae6-4074-bdd0-5e17ae3b5f11</a>. Formatted as a Frictionless Data package
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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