1,724,379 research outputs found
Mir-3928 in Saliva as Potential Biomarker for Head and Neck Squamous Cell Carcinoma
No full textObjectives: To determine whether salivary miR-3928 can be used for early head and neck cancer diagnostic purpose.
Methods: The expression level of human salivary miR-3928 was investigated in 230 supernatant saliva samples. These samples were collected from head and neck squamous cell carcinoma (HNSCC) patients (n = 150) and healthy individuals (n = 80). Quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect the expression level of the miRNA.
Results: Total RNA was isolated in average 160 ng/µl from 500 µl of supernatant saliva. Overall, miR-3928 expression was revealed to be correlated with HNC. We found that salivary miR-3928 was significantly down regulated in supernatant saliva of HNC patients (P< 0.05).
Conclusions: This study reported that down-regulate of miR-3928 could be used as salivary biomarker for HNSCC detection.No Full Tex
Union Pacific (UP) 3928
No full textA photograph postcard showing Union Pacific (UP) 3928, 4-6-6-4, Green River, WY
Down-Regulation of miR-3928 Promoted Osteosarcoma Growth
No full textBackground: Osteosarcoma is the most common primary bone malignancy in children and young adults. Most failures of osteosarcoma treatment were due to resistance to chemotherapy. Development of new therapy required elucidation underlying molecular mechanism. Many miRNAs have been proved to be involved in the pathogenesis of osteosarcoma. Methods: MiR-3928 expression level was assayed by qRT-PCR. MiRNA mimics or ASO were transfected for up-regulation or down-regulation of miR-3928 expression. Cell proliferation was assayed by formazan test. Apoptosis and cell cycle were assayed by FACS. MiR-3928 targeted genes were predicated by bioinformatics algorithm (TargetScanHuman). The correlation between targeted gene and miR-3928 was analyzed by Pearson's correlation coefficient analysis. Results: MiR-3928 was down-regulated in osteosarcoma tissues. Over-expression of miR-3928 inhibited tumor growth, induced cell apoptosis, increased the percent of cells in G1 phrase and decreased the percent of cells in S phrase. Down-regulation of miR-3928 promoted cell proliferation. ERBB3, IL-6R and CDK6 may be the targeted genes of miR-3928. Conclusions: Down-expression of miR-3928 in osteosarcoma promoted tumor growth by targeting ERBB3, IL-6R and CDK6. MiR-3928 may be a potential therapy target worth further investigation
Block Card 3928 Catawba Street
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 3928 Catawba Street (Toledo, Ohio) | Bungalow Style | North Toledo (Toledo, Ohio) | Creekside Addition (Toledo, Ohio
Block Card 3928 Burton Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 3928 Burton Avenue (Toledo, Ohio) | Gabled Ell | Vernacular Victorian Style | Almeda Heights Second Addition (Toledo, Ohio) | Willys Park area (Toledo, Ohio) | West Toledo (Toledo, Ohio
MicroRNA 3928 Suppresses Glioblastoma through Downregulation of Several Oncogenes and Upregulation of p53
No full textGlioblastoma (GBM) is the most frequent and lethal primary malignant brain tumor. Despite decades of research, therapeutic advances that significantly prolong life are non-existent. In recent years, microRNAs (miRNAs) have been a focus of study in the pathobiology of cancer because of their ability to simultaneously regulate multiple genes. The aim of this study was to determine the functional and mechanistic effects of miR-3928 in GBM both in vitro and in vivo. To the best of our knowledge, this is the first article investigating the role of miR-3928 in GBM. We measured endogenous miR-3928 expression levels in a panel of patient-derived GBM tissue samples and cell lines. We found that GBM tissue samples and cell lines express lower levels of miR-3928 than normal brain cortex and astrocytes, respectively. Therefore, we hypothesized that miR-3928 is a tumor suppressive microRNA. We verified this hypothesis by showing that exogenous expression of miR-3928 has a strong inhibitory effect on both cell growth and invasiveness of GBM cells. Stable ex vivo overexpression of miR-3928 in GBM cells led to a reduction in tumor size in nude mice xenografts. We identified many targets (MDM2, CD44, DDX3X, HMGA2, CCND1, BRAF, ATOH8, and BMI1) of miR-3928. Interestingly, inhibition of the oncogene MDM2 also led to an upregulation of wild-type p53 expression and phosphorylation. In conclusion, we find that miR-3928, through the downregulation of several oncogenes and upregulation and activation of wild-type p53, is a strong tumor suppressor in GBM. Furthermore, the fact that miR-3928 can target many important dysregulated proteins in GBM suggests it might be a “master” regulatory microRNA that could be therapeutically exploited
Block Card 3928 Crary Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Dwelling | 3928 Crary Drive (Toledo, Ohio) | Cape Cod Style | Keystone Addition (Toledo, Ohio) | Deveaux Area (Toledo, Ohio) | West Toledo area (Toledo, Ohio
Beyond recurrent costs: an institutional analysis of the unsustainability of donor-supported reforms in agricultural extension
Full text linkInternational donors have spent billions of dollars over the past four decades in developing and/or reforming the agricultural extension service delivery arrangements in developing countries. However, many of these reforms, supported through short-term projects, became unsustainable once aid funding had ceased. The unavailability of recurrent funding has predominantly been highlighted in the literature as the key reason for this undesirable outcome, while little has been written about institutional factors. The purpose of this article is to examine the usefulness of taking an institutional perspective in explaining the unsustainability of donor-supported extension reforms and derive lessons for improvement. Using a framework drawn from the school of institutionalism in a Bangladeshi case study, we have found that a reform becomes unsustainable because of poor demands for extension information and advice; missing, weak, incongruent, and perverse institutional frameworks governing the exchange of extension goods (services); and a lack of institutional learning and change during the reform process. Accordingly, we have argued that strategies for sustainable extension reforms should move beyond financial considerations and include such measures as making extension goods (services) more tangible and monetary in nature, commissioning in-depth studies to learn about local institutions, crafting new institutions and/or reforming the weak and perverse institutions prevailing in developing countries. We emphasize the need to address three categories of institutions – regulative, normative, and cultural-cognitive – and call for an alignment among them. We further argue that, in order to be sustainable, a reform should take a systemic approach in institutional capacity building and, for this to be possible, adopt a long-term program approach, as opposed to a short-term project approach
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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