1,724,986 research outputs found
GSK-3β was a candidate target of miRNA-3646.
No full texta Four complementary sites of miR-3646 in GSK-3β 3’UTR were predicted by bioinformatics analysis. b Relative mRNA and protein expression of GSK-3β in MDA-MB-231/S and MDA-MB-231/Doc cells. c Relative mRNA and protein expression of GSK-3β in MDA-MB-231/S cells transfected with miR-3646 mimics, NC and blank control. d Relative mRNA and protein expression of GSK-3β in MDA-MB-231/Doc cells transfected with miR-3646 inhibitors, NC and blank control.</p
Effect of miR-3646 mimics and miR-3646 inhibitors on the sensitivity of breast cancer cell lines to Doc.
No full texta,c The IC50 value of Doc was determined after MDA-MB-231/S or MCF-7/S cells were transfected with miR-3646 mimics, NC or blank control for 48h using MTT assay (*Pb,d After MDA-MB-231/Doc or MCF-7/S cells were transfected with miR-3646 inhibitors, NC or blank control for 48h, theIC50 value of Doc was determined by MTT assay (*P<0.05).</p
LncRNA DANCR reduces anoxia-induced rat myocardial cell line H9c2 injury by targeting miR-3646
No full textObjective To study the role of long non-coding RNA (lncRNA) differentiation antagonizing non-coding RNA (DANCR) in myocardial ischemia and the targeted regulation of microRNA-3646 (miR-3646). Methods H9c2 cells(rat cardiomyocytes cell line)were separated into 6 groups: control group, anoxia group, pc-DANCR (DANCR over-expression) group, pc-NC (DANCR over-expression control) group, miR-3646 mimic (miR-3646 mimicry) group, mimic NC (miR-3646 mimic control) group, and pc-DANCR+miR-3646 mimic group; RT-qPCR method was implemented to measure the expression of lncRNA DANCR and miR-3646 to determine the transfection effect; Flow cytometry was performed to measure the rate of apoptosis; CCK-8 assay was implemented to cell measure survival; Electron microscopy was applied to observe the structural injury of H9c2 cells; Western blot was performed to measure the expression of activated cleaved caspase-3 (cleaved caspase-3) and heme oxygenase-1 (Ho-1). Dual-luciferase experiments were performed to verify the targeting relationship of miR-3646 with lncRNA DANCR and Ho-1. RNA interference Ho-1 (ShHo-1) and pc-DANCR were co-transfected into H9c2 cells, and the cell viability was measured. Results Compared with the control group, the injury and apoptosis of H9c2 cells in the anoxia group were serious, the survival rate and the expression of lncRNA DANCR were decreased, and the expression of miR-3646 was increased (PPPPConclusions LncRNA DANCR can down-regulate the expression of miR-3646 and promote the expression of Ho-1 to alleviate anoxia-induced H9c2 cells injury and improve cell survival
Block Card 3646 Torrance Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 3646 Torrance Drive (Toledo, Ohio) | Colonial Revival Style | Almeda Heights (Toledo, Ohio) | Willys Park area (Toledo, Ohio) | West Toledo (Toledo, Ohio
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Characteristics of mutant 3646.
No full text<p>(A) Phenotype of six week old Wassilewskija (WS) and activation tag mutant 3646; (B) Location of the T-DNA insert (inverted triangle) containing the transposon (grey square) between At5g65040 (<i>IRM1</i>) and At5g65050. Black triangles at the end of the genes indicate the gene orientation. The distance from a gene to the T-DNA is indicated below the horizontal line. Short lines above the genes represent the position of primers used for quantitative RT-PCR analysis. Diagram is not drawn to scale; (C) Quantitative RT-PCR analysis of the two genes flanking the T-DNA. Values are the means ± standard deviation of three biological replicates. The star indicates a significant difference between bars within a pair (Independent-samples <i>t</i>-test, <i>P</i><0.05).</p
MiR-3646 accelerates inflammatory response of Ang II-induced hVSMCs via CYP2J2/EETs axis in hypertension model
No full textBackground Inflammatory response of human vascular smooth muscle cells (hVSMCs) is a driving factor in hypertension progression. It has been reported that miR-3646 was significantly up-regulated in serum samples from patients with coronary artery disease and acute myocardial infarction mice. However, its role and underlying molecular mechanism related to inflammatory response of angiotensin II (Ang II)-induced hVSMCs remain unclear. Objective We aimed to explore the potential molecular mechanisms related to inflammatory response of angiotensin II (Ang II)-induced hVSMCs. Methods Ang II–induced hypertension model was established after hVSMCs treated with 1 μM Ang II at 24 h. The interaction between microRNA 3646 (miR-3646) and cytochrome P450 2J2 (CYP2J2) was assessed by dual-luciferase reporter gene assay. MTS assay, Lipid Peroxidation MDA Assay Kit, ELISA, Western blot, and qRT-PCR were performed to examine viability, malondialdehyde (MDA) level, inflammatory cytokine levels, and the level of genes and proteins. Results Our findings illustrated that miR-3646 was up-regulated but CYP2J2 was down-regulated in Ang II–induced hVSMCs. Mechanically, miR-3646 negatively targeted to CYP2J2 in Ang II–induced hVSMCs. These findings indicated that miR-3646 regulated inflammatory response of Ang II–induced hVSMCs via targeting CYP2J2. Moreover, functional researches showed that CYP2J2 overexpression alleviated inflammatory response of Ang II–induced hVSMCs via epoxyeicosatrienoic acids/peroxisome proliferator-activated receptor-γ (EETs/PPARγ) axis, and miR-3646 aggravated inflammatory response of Ang II–induced hVSMCs via mediating CYP2J2/EETs axis. Conclusion MiR-3646 accelerated inflammatory response of Ang II–induced hVSMCs via CYP2J2/EETs axis. Our findings illustrated the specific molecular mechanism of miR-3646 regulating hypertension
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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