1,721,249 research outputs found
Y-27632 and Y-33075 treatments increase migration in human TWNT-4 and primary murine HSCs.
No full text(A) Effect of ROCK inhibitors Y-27632 and Y-33075 on wound closure in activated human TWNT-4 cells measured at 4, 8 and 24 hours (n = 3). (B) Representative images of the wound healing assay in TWNT-4 cells at 4, 8 and 24 hours after treatment with the ROCK inhibitors. The scale bars apply for all images and the length represents 200μm. (C) Effect of ROCK inhibitors Y-27632 and Y-33075 on wound closure in primary culture-activated FVB/NJ cells measured at 4, 8 and 24 hours (n = 3). (D) Representative images of the wound healing assay taken 4, 8 and 24 hours after treatment of FVB/NJ cells with ROCK inhibitors (scale bars, 200μm). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.</p
Y-27632 and Y-33075 treatments reduce contraction in human TWNT-4 and primary murine HSCs.
No full text(A) Schematic depiction of the RhoA-ROCK pathway including mechanism of action of the ROCK inhibitors Y-27632 and Y-33075. (B) Chemical structure of ROCK inhibitors Y-27632 and Y33075. (C) Effect of Y-27632 and Y-33075 on collagen gel contraction of activated human TWNT-4 cells (n = 3). (D) Representative images of the contraction assay in TWNT-4 cells after incubation with the ROCK inhibitors for 24 hours. (E) Effect of Y-27632 and Y-33075 on collagen gel contraction of culture-activated primary murine FVB/NJ cells (n = 3). (F) Representative images of gel contraction in FVB/NJ cells 24 hours after treatment with the ROCK inhibitors. (G) Protein expression levels of phospho-MLC and MLC in TWNT-4 after 24 hour incubation with the ROCK inhibitors. (H) Ratio of phospho-MLC to MLC in TWNT-4 cell lysates after treatment with ROCK inhibitors for 24 hours (n = 2). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.</p
The non-selective Rho-kinase inhibitors Y-27632 and Y-33075 decrease contraction but increase migration in murine and human hepatic stellate cells
No full textBACKGROUND: The Rho-kinase ROCK II plays a major role in the activation of hepatic stellate cells (HSC), which are the key profibrotic and contractile cells contributing to the development of chronic liver disease. Inhibition of ROCK II ultimately blocks the phosphorylation of the myosin light chain (MLC) and thus inhibits stress fibre assembly and cell contraction. We investigated the effects of the ROCK inhibitors Y-33075 as well as Y-27632 in murine and human hepatic stellate cells. METHODS: Primary isolated HSC from FVB/NJ mice and the immortalized human HSC line TWNT-4 were culture-activated and incubated with Y-27632 and Y-33075 (10nM to 10μM) for 24h. Protein expression levels were analyzed by Western Blots and transcriptional levels of pro-fibrotic markers and proliferative markers were evaluated using real-time qPCR. Migration was investigated by wound-healing assay. Proliferation was assessed by BrdU assay. Contraction of HSC was measured using 3D collagen matrices after incubation with Y-27632 or Y-33075 in different doses. RESULTS: Both Rho-kinase inhibitors, Y-27632 and Y-33075, reduced contraction, fibrogenesis and proliferation in activated primary mouse HSC (FVB/NJ) and human HSC line (TWNT-4) significantly. Y-33075 demonstrated a 10-times increased potency compared to Y-27632. Surprisingly, both inhibitors mediated a substantial and unexpected increase in migration of HSC in FVB/NJ. CONCLUSION: ROCK inhibition by the tested compounds decreased contraction but increased migration. Y-33075 proved more potent than Y27632 in the inhibition of contraction of HSCs and should be further evaluated in chronic liver disease
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Y-27632 and Y-33075 treatments reduce fibrosis in human TWNT-4 and primary mouse HSCs.
No full text(A) Western blots of Col1a1, p-Moesin, Moesin and GAPDH using TWNT-4 cell lysates after 24 hour incubation with the ROCK inhibitors. (B) Quantification of protein expression of Col1a1 after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (C) Quantification of protein expression of Moesin after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (D) Quantification of protein expression of pMoesin after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (E) Western Blots of Col1a1, p-Moesin, Moesin and GAPDH using cell lysates of primary FVB/NJ cells after incubation with ROCK inhibitors for 24 hours. (F). Quantification of protein expression of Col1a1 after treatment of FVB/NJ cells with ROCK inhibitors for 24 hours (n = 3). (G) Quantification of protein expression of Moesin after treatment of FVB/NJ cells with ROCK inhibitors for 24 hours (n = 3). (H) Quantification of protein expression of p-Moesin after treatment of FVB/NJ cells with ROCK inhibitors for 24 hours (n = 3). (I) TWNT-4 mRNA expression levels of the pro-fibrotic marker Col1a1 and Tgfβ (corrected to 18s as a housekeeping gene) after 24h incubation with ROCK inhibitors Y-27632 and Y-33075 (n = 3). (J) FVB/NJ mRNA expression levels of the pro-fibrotic marker Col1a1 and Tgfβ (corrected to 18s as a housekeeping gene) after 24h incubation with ROCK inhibitors Y-27632 and Y-33075 (n = 3). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.</p
La gran recesión y los sistemas autonómicos de protección social
No full textCon autorización de la editorial para este capítulo.Queremos agradecer el apoyo a este trabajo del Ministerio de Economía y Competitividad a través de la financiación del proyecto "Capacidad y Estrategias de Reforma frente a la Crisis Fiscal en los Sectores Públicos y las Políticas de Bienestar Autonómicas: Condicionantes y Trayectorias de Cambio" (CRISAUT), CSO2012-33075.Peer reviewe
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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