1,726,875 research outputs found
Additional file 1 of Treg cells-derived exosomes promote blood-spinal cord barrier repair and motor function recovery after spinal cord injury by delivering miR-2861
No full textAdditional file 1: Fig. S1. Validation of the knockout efficiency of Treg cells in the spinal cord of mice. A: The knockout efficiency of Treg cells was verified by immunofluorescence staining. B The knockout efficiency of Treg cells was verified by western blotting. Fig. S2. Dil-labeled exosomes are phagocytosed by bEND.3 cells. Fig. S3. Selection and validation of miR-2861. A: The intersection of 4 samples from the GEO database yielded 5 miRNAs containing miR-2861. B Relative expression of miR-2861 in exosome-treated bEND.3 cells (n = 3)
Astronomische Nachrichten, nos 2861-2868
No full textR. R. Astronomische Nachrichten, nos 2861-2868. In: Bulletin astronomique, tome 6, 1889. pp. 45-48
Block Card 2861 Manchester Boulevard
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 2861 Manchester Boulevard (Toledo, Ohio) | attached garage | Dwelling | Old Orchard Addition, Fifth Extension (Toledo, Ohio) | Westgate Area (Toledo, Ohio
CRISPR activation identifies a novel miR-2861 binding site that facilitates the osteogenesis of human mesenchymal stem cells
No full textAbstract We investigated the regulation of histone deacetylases (HDACs) by miR-2861 in the osteoblastic differentiation of human mesenchymal stem cells (MSCs) and miR-2861 binding site by CRISPR activation (CRISPRa). Transfection of miR-2861 into human MSCs was performed and the effect on osteoblast differentiation was analyzed. Using catalytically inactive Cas12a, the CRISPRa system induced targeted overexpression of endogenous miRNA and repressed the luciferase activities of reporters that contained functional miRNA target sites. The delivery of miR-2861 into MSCs enhanced osteoblast differentiation by decreased expressions of the HDAC1, 4 and 5 genes. The mechanism of HDAC5 repression by miR-2861 in humans has not been fully elucidated. To this end, the HDAC5 mRNA sequence was analyzed and a putative primate-specific miR-2861 binding site was identified in the 3’ untranslated region (3’-UTR). CRISPRa was applied to validate the putative binding site and an increase in endogenous miR-2861 was found to repress the expression of a reporter that contained the novel miR-2861 binding site. The delivery of miR-2861 to human MSCs enhanced osteoblast differentiation. In the 3’-UTR, the HDAC5 repression was mediated by the miR-2861 binding site, and miR-2861 promoted osteoblast differentiation via the inhibition of HDAC5 through a primate-specific miRNA binding site. Therefore, miRNAmiR-2861 with the CRISPRa methods might be a good biomaterial for osteogenesis augmentation
Linked collectors and determiners for: A new stiletto fly genus from South America (Diptera: Therevidae: Agapophytinae).
No full textNatural history specimen data linked to collectors and determiners held within, "A new stiletto fly genus from South America (Diptera: Therevidae: Agapophytinae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/e3adbec8-2861-49f8-b7a2-39f1bdcbb493">https://bionomia.net/dataset/e3adbec8-2861-49f8-b7a2-39f1bdcbb493</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/e3adbec8-2861-49f8-b7a2-39f1bdcbb493">https://gbif.org/dataset/e3adbec8-2861-49f8-b7a2-39f1bdcbb493</a>. Formatted as a Frictionless Data package
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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