1,721,299 research outputs found
Genes of pyruvate catabolism and hydrogen synthesis in Clostridium thermocellum ATCC 27405
No full textClostridium thermocellum is a gram-positive, acetogenic, thermophilic, anaerobic bacterium that degrades cellulose and carries out mixed product fermentation, catabolising glucose to acetate, lactate, and ethanol under various growth conditions, with the concomitant release of H, and CO2. We have begun to investigate H2-production by C. thermocellum ATCC 27405 cultured in media containing different carbon sources, including glucose, cellobiose, crystalline cellulose, a-cellulose, paper, and delignified wood fibres. We have detected formate synthesis by C. thermocellum ATCC 27405 cultured on both cellobiose and a-cellulose. While formate synthesis has been reported for one strain of Clostridium thermocellum (strain I-1-B), numerous fermentation studies of C. thermocellum 27405 have failed to detect the presence of formate. Formate production was detected throughout growth, and pyruvate:formate lyase (PFL) enzyme activity was detected in late log and stationary phase in extracts of C. thermocellum cultured on cellobiose. Formate synthesis competes with the production of hydrogen (H2) as a fermentation end-product, and thus negatively impacts H2 yields. Bioinformatic analyses of the C. thermocellum genome identified genes encoding key enzymes in pyruvate catabolism pathways, including two putative lactate dehydrogenases (LDH), one PFL. four pyruvate:formate lyase activating enzymes, and at least three putative pyruvate:ferredoxin oxidoreductase (POR) or POR-like enzymes. Our data suggests that hydrogen may be generated through the action of either a Ferredoxin (Fd)-dependent NiFe hydrogenase, often referred to as "Energy-converting Hydrogenases" (Ech), or via NAD(P)H-dependent Fe-only hydrogenases which would permit H2 production from NADH generated during the glyeeraldehyde-3-phosphate dehydrogenase reaction. Furthermore, our findings show the presence of multiple genes putatively encoding NADH:Fd oxidoreductase; suggesting a possible mechanism in which electrons could be transferred from NADH to ferredoxin. The elucidation of pyruvate catabolism pathways and mechanisms of H2 synthesis is the first step in developing strategies to increase hydrogen yields from biomass. My studies have outlined the likely pathways leading to hydrogen synthesis in C, thermocellum ATCC 27405. The actual functional roles of these gene products during pyruvate catabolism and in H2 synthesis remain to be elucidated and will need to be confirmed using both expression analysis and protein characterization
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
A New Restriction Endonuclease, CthII, from Clostridium thermocellum ATCC 27405
No full textClostridium thermocellum(ATCC 27405) 2g(wet weight)으로부터 새로운 type II 제한효소인 CthII를 분리하여, 그 효소의 생화학적 특성을 연구하였다. CthII는 DEAE-Cellulose와 Phosphocellulose chromatography를 통하여 분리하였다. 두번째 column인 Phosphocellulose column을 통해 나온 fraction들 중 이미 발견된 CthI peak보다 나중의 fraction들에서 이 효소가 발견되었다. CthII는 5-CC (A/T) GG-3를 인식하며 화살표로 표시한 위치를 절단하는 효소임이 밝혀졌다. 이 효소의 반응을 위한 최적온도는 55℃였으며, Mg2＋ 존재하에 pH 8과 pH 9 사이에서 최대활성을 보여주었다. 또한 이 효소는 NaCI이 없는 상태에서 정상적인 효소 활성을 보여주었고, NaCl 농도가 100 mM 이상일 때는 그 효소 활성이 감소됨을 관찰하였다. CthII는 BstNI과 ZanI과 같이 dcm-methylated DNA(5-(A/T)GG-3)도 정상적으로 절단할 수 있는 특성도 있음이 발견되었다
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
- …
