1,731,810 research outputs found
New Real-Time PCR Assay Using Allelic Discrimination for Detection and Differentiation of Equine Herpesvirus-1 Strains with A 2254 and G 2254 Polymorphisms
No full textInternational audienceA single-nucleotide polymorphism (A 2254 or G 2254 ) in open reading frame 30 (ORF30) has been linked to the neuropathogenic phenotype of equine herpesvirus-1 (EHV-1). Identification of this polymorphism led to the development of a real-time PCR (rPCR) assay using allelic discrimination (E 2 ) to distinguish between potentially neuropathogenic and nonneuropathogenic EHV-1 strains (G. P. Allen, J. Vet. Diagn. Invest. 19:69–72, 2007). Although this rPCR assay can detect and genotype EHV-1 strains, subsequent studies demonstrated that it lacks the sensitivity for the routine detection of viral nucleic acid in clinical specimens. Therefore, a new allelic discrimination EHV-1 rPCR assay (E 1 ) was developed by redesigning primers and probes specific to ORF30. The E 1 and E 2 rPCR assays were evaluated using 76 archived EHV isolates and 433 clinical specimens from cases of suspected EHV-1 infection. Nucleotide sequence analysis of ORF30 was used to confirm the presence of EHV-1 and characterize the genotype (A 2254 or G 2254 ) in all archived isolates plus 168 of the clinical samples. The E 1 assay was 10 times more sensitive than E 2 , with a lower detection limit of 10 infectious virus particles. Furthermore, all A 2254 and G 2254 genotypes along with samples from three cases of dual infection (A 2254 +G 2254 ) were correctly identified by E 1 , whereas E 2 produced 20 false dual positive results with only one actual mixed A 2254 +G 2254 genotype confirmed. Based on these findings, E 1 offers greater sensitivity and accuracy for the detection and A/G 2254 genotyping of EHV-1, making this improved rPCR assay a valuable diagnostic tool for investigating outbreaks of EHV-1 infection
Block Card 2254 Drummond Road
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: English Cottage Style | 2254 Drummond Road (Toledo, Ohio) | Dwelling | Barton Place Addition (Toledo, Ohio) | Old Orchard Area (Toledo, Ohio
Block Card 2254 Carriage Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: attached garage | Ranch houses | 2254 Carriage Drive (Toledo, Ohio) | Dwelling | Country Squire Estates (Toledo, Ohio) | Reynolds Corners Area (Toledo, Ohio
Block Card 2254 Forrer Street
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Folk House Style | 2254 Forrer Street (Toledo, Ohio) | Dwelling | Morfoot's Addition (Toledo, Ohio) | Scott Park Area (Toledo, Ohio) | Gable front house
Briefing To The Security Council Pursuant To Resolution 2254(2015)
No full textBriefing To The Security Council Pursuant To Resolution 2254(2015
Block Card 2254 Kneer Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: 2254 Kneer Drive (Toledo, Ohio) | Heatherwoods (Toledo, Ohio) | South Toledo Area (Toledo, Ohio) | Ranch houses | Dwellin
Campbell, Arthur L. (SC 2254)
Get PDFFinding aid only for Manuscripts Small Collection 2254. Correspondence between Arthur L. Campbell and H.L. Mencken related to Campbell\u27s newspaper column How\u27s Your English Today and his requests for permission to quote Mencken in his column. Mencken\u27s last two letters were written by his secretary owing to his poor health
Block Card 2254 Goddard Road
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Split-Level Style | 2254 Goddard Road (Toledo, Ohio) | Dwelling | Barton Place Addition (Toledo, Ohio) | Old Orchard Area (Toledo, Ohio
Block Card 2254 Bucklew Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Dwelling | 2254 Bucklew Drive (Toledo, Ohio) | Ranch houses | Towne Acres (Toledo, Ohio) | Greenwood Area (Toledo, Ohio) | West Toledo Area (Toledo, Ohio
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