1,721,375 research outputs found
Monitoring the plant epiphyte Methylobacterium extorquens DSM 21961 by real-time PCR and its influence on the strawberry flavor
No full textBesides its influence on plant growth and health, plant-associated bacteria exert an impact on fruit quality. Methylotrophic bacteria can enhance the biosynthesis of strawberry flavor compounds, especially the two furanoid compounds 2,5-dimethyl-4-hydroxy-2H-furanone (DMHF) and 2,5-dimethyl-4-methoxy-2H-furanone in vitro. Here, we report the selection and characterization of Methylobacterium extorquens DSM 21961, a strain that was able to enhance the furanone content ad planta under greenhouse conditions. For monitoring the colonization of strawberry plants, a strain-specific quantification system for M. extorquens DSM 21961 was developed. Specificity, linear range and quantitative limit of the system were shown, and successful application was demonstrated in a monitoring experiment of M. extorquens DSM 21961 on strawberry leaves under greenhouse conditions. Furthermore, the quantification of DMHF in strawberry fruits via GC indicated an increased biosynthesis of this compound in strawberry plants. The colonization behavior analyzed by confocal laser scanning microscopy using GFP-tagged cells revealed high colonization of the upper and the lower leaf surfaces, with a specific accumulation of bacterial cells on trichomes. The results support a biotechnological application of this promising flavor-stimulating agentOutre son influence sur la croissance et la sant\ue9 des v\ue9g\ue9taux, les bact\ue9ries associ\ue9es aux v\ue9g\ue9taux influent sur la qualit\ue9 des fruits. Les bact\ue9ries m\ue9thylotrophes peuvent stimuler la biosynth\ue8se de compos\ue9s \ue0 saveur de fraise in vitro, surtout les deux compos\ue9s furano\uefdes 2,5 dim\ue9thyl 4 hydroxy 2H furanone (DMHF) et 2,5 dim\ue9thyl 4 m\ue9thoxy 2H furanone. Dans le pr\ue9sent article, nous faisons \ue9tat du choix et de la caract\ue9risation de la bact\ue9rie Methylobacterium extorquens DSM 21961, une souche capable d\u2019accro\ueetre le contenu en furanone dans la plante cultiv\ue9e en serre. Pour suivre la colonisation des fraisiers, on a mis au point un syst\ue8me de quantification propre \ue0 la souche M. extorquens DSM 21961. Ces travaux ont permis de montrer la sp\ue9cificit\ue9, la lin\ue9arit\ue9 et la limite de quantification du syst\ue8me et de d\ue9montrer une application r\ue9ussie dans le cas d\u2019une exp\ue9rience de suivi de M. extorquens DSM 21961 sur des feuilles de fraisiers cultiv\ue9s en serre. De plus, le dosage du DMHF dans les fraises par chromatographie en phase gazeuse indique une biosynth\ue8se accrue de ce compos\ue9 dans les fraisiers. Le comportement de colonisation, analys\ue9 par microscopie confocale \ue0 balayage laser au moyen de cellules marqu\ue9es d\u2019une prot\ue9ine \ue0 fluorescence verte, r\ue9v\ue8le une forte colonisation sur les faces sup\ue9rieure et inf\ue9rieure des feuilles et une accumulation de bact\ue9ries sp\ue9cifiquement sur les trichomes. Ces r\ue9sultats appuient une application biotechnologique de cet agent rehausseur de saveur prometteur.Peer reviewed: YesNRC publication: Ye
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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