1,726,322 research outputs found
miR-2188 duplex and morpholino injections.
No full text<p>A) A miRNA duplex and a MO<sub>miR-2188</sub> were designed to over express and knockdown miR-2188 in zebrafish embryos, respectively. Duplex microinjections were performed in one cell stage embryos with rhodamine to visualize the injection process. Approximately 1000 pL of each duplex were injected. 2 µM of each duplex were chosen as the working concentration for the injections, as this duplex concentration did not induce high mortality rates or unspecific side effects. B) qPCR quantification of miR-2188 showed that miR-2188 duplex injection increased miR-2188 expression by 40 fold and MO<sub>miR-2188</sub> injection decreased miR-2188 expression by 12 fold. C) qPCR quantification of <i>Nrp2a</i> levels in 48 hpf embryos after over expression and knockdown of miR-2188 showed that this target was down regulated 1.6 fold after miR-2188 over expression and was up regulated 2 fold after miR-2188 knockdown.</p
Revision of Milichiella Giglio-Tos (Diptera, Milichiidae) 2188
No full textBrake, Irina (2009): Revision of Milichiella Giglio-Tos (Diptera, Milichiidae) 2188. Zootaxa 2188 (1): 1-166, DOI: 10.11646/zootaxa.2188.1.1, URL: https://biotaxa.org/Zootaxa/article/view/zootaxa.2188.1.
ISH of <i>Nrp2a</i> after miR-2188 deregulation.
No full text<p>A) After injection of miR-2188 duplex, <i>Nrp2a</i> detection decreased between the somite boundaries at 24 hpf. On the other hand, in miR-2188 morphants, <i>Nrp2a</i> detection was more prounounced throughout the trunk and tail region, between the somite boundaries, when compared with the control. B) At 48 hpf and after miR-2188 overexpression, <i>Nrp2a</i> was not detected in the blood vessels or in the PCV when compared with the control embryos. On the other hand, miR-2188 morphants express <i>Nrp2a</i> in the trunck and tail, especially between the somite boundaries, which is not detected in 48 hpf control embryos. All embryos are in lateral view, anterior to left. Arrows indicate hybridization signal. Microscopic pictures were taken with a 6,3x magnification.</p
2188. Hermannus Contractus, moine de Reichenau
No full text2188. Hermannus Contractus, moine de Reichenau. In: Molinier Auguste. Les Sources de l'histoire de France - Des origines aux guerres d'Italie (1494). II. Époque féodale, les Capétiens jusqu'en 1180. Paris : A. Picard et fils, 1902. pp. 306-307
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Mill Creek Baptist Church - Monroe County, Kentucky (SC 2188)
Full text linkFinding aid and scan (Click on Additional Files below) for Manuscripts Small Collection 2188. Record book of Mill Creek Baptist Church, Monroe County, Kentucky. Includes rules of decorum, minutes of meetings, and membership lists
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