1,746,684 research outputs found
Commento agli artt. 2114-2117
No full textL'A. commenta gli artt. 2114-2117 c.c. alla luce della più recente giurisprudenz
Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures
Get PDFVesivirus 2117 is an adventitious agent that in 2009, was identified as a contaminant of CHO cells propagated in bioreactors at a pharmaceutical manufacturing plant belonging to Genzyme. The consequent interruption in supply of Fabrazyme and Cerezyme (drugs used to treat Fabry and Gaucher disease respectively), caused significant economic losses. Vesivirus 2117 is a member of the Caliciviridae; a family of small icosahedral viruses encoding a positive sense RNA genome. We have used cryo-electron microscopy and three dimensional image reconstruction to calculate a structure of vesivirus 2117 virus like particles as well as feline calicivirus and a chimeric sapovirus. We present a structural comparison of several members of the Caliciviridae, showing that the distal P domain of vesivirus 2117 is morphologically distinct from that seen in other known vesivirus structures. Furthermore, at intermediate resolutions we found a high level of structural similarity between vesivirus 2117 and Caliciviridae from other genera, such as sapovirus and rabbit haemorrhagic disease virus. Phylogenetic analysis confirms vesivirus 2117 as a vesivirus closely related to canine vesiviruses. We postulate that morphological differences in virion structure seen between vesivirus clades may reflect differences in receptor usage
Serological and molecular investigation of 2117-like vesiviruses in cats
No full textVesivirus 2117 was first discovered as a contaminant in Chinese hamster ovary (CHO) cell cultures used for human drug production. Similar vesiviruses (VeVs) have been detected recently in dogs. In order to address the hypothesis that cats may also be exposed to 2117-like VeVs, in this study, we screened 236 feline sera using an enzyme-linked immunosorbent assay (ELISA) based on a recombinant VP1 protein from the canine VeV Bari/212/07/ITA. IgG antibodies against the 2117-like VeV were detected in 37.3% of the sera tested. Also, by screening cat faecal specimens, the RNA of a 2117-like VeV was detected in a clinically healthy cat
Serological and molecular investigation of 2117?like vesivirusesin cats
No full textAbstract Vesivirus 2117 was first discovered as a contaminantin Chinese hamster ovary (CHO) cell culturesused for human drug production. Similar vesiviruses (VeVs)have been detected recently in dogs. In order to address thehypothesis that cats may also be exposed to 2117-like VeVs,in this study, we screened 236 feline sera using an enzymelinkedimmunosorbent assay (ELISA) based on a recombinantVP1 protein from the canine VeV Bari/212/07/ITA. IgGantibodies against the 2117-like VeV were detected in 37.3%of the sera tested. Also, by screening cat faecal specimens,the RNA of a 2117-like VeV was detected in a clinicallyhealthy cat
McClary, Herbert Brent, 1881-1952 (SC 2117)
Get PDFFinding aid and scans (Click on Additional Files below) for Manuscripts Small Collection 2117. Brief history of Auburn, Logan County, Kentucky, and related correspondence
Seroprevalence for 2117-like vesiviruses in Italian household dogs
Get PDFIn 2003, a novel calicivirus, the vesivirus (VeV) strain 2117, was identified incidentally as a contaminant in Chinese Hamster Ovary (CHO) cell cultures by a German pharmaceutical company. Similar contaminations have been documented in three additional episodes, in bio-reactors used for production of recombinant drugs. More, recently 2117-like VeVs have also been identified at high prevalence in the stools from asymptomatic kennel dogs and only sporadically in diarrhoeic and healthy household dogs. In this study, antibodies for 2117-like viruses were detected in 21.5% of sera from household dogs, indicating that they are common viruses in the canine host
Block Card 2117 Marlow Road
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Bungalow Style | Dwelling (Toledo, Ohio) | 2117 Marlow Road (Toledo, Ohio) | Dauer Gardens Addition (Toledo, Ohio) | Deveaux Area (Toledo, Ohio) | West Toledo (Toledo, Ohio
- …
