1,721,809 research outputs found
Bafilomycin A1 treatment allows 19606 to replicate in macrophages.
No full text(A) J774A.1 macrophages were infected with GFP-19606 and treated with the proton pump V-ATPase inhibitor bafilomycin A1. Total numbers of intracellular CFU were determined at different times pi in treated and non-treated cells. Statistical analyses were performed by two-way ANOVA-test, ** < 0.0021, **** < 0.0001. (B) Representative images of cells infected with GFP-19606 (green) and incubated with or without bafilomycin A1 at 6 hpi are shown. Cell nuclei were stained with DAPI (blue) and LAMP1 with specific antibody (red). Bars: 20 μm.</p
Efecto antibacteriano in vitro de los aceites escenciales de Cinnamomum verum (CANELA) y Citrus limon (LIMÓN) sobre, Acinetobacter baumannii ATCC 19606 y Klebsiella pneumoniae ATCC 700603 – Jaén, 2023
Get PDFLa resistencia antibacteriana, generalmente se ocasiona por el uso continuo de agentes antibacterianos que producen agentes mutagénicos, ocasionando un aumento de variabilidad celular. Teniendo como objetivo principal determinar el efecto antibacteriano in vitro de los aceites esenciales de Cinnamomum verum (canela) y Citrus limon (limón) sobre Acinetobacter baumannii ATCC 19606 y Klebsiella pneumoniae ATCC 700603; los aceites esenciales fueron obtenidos por el método de hidrodestilación, utilizando el método de dilución con alcohol para las concentraciones de 100%; 75%; 50% y 25%; las pruebas de sensibilidad fueron determinadas por la técnica de discos de difusión; el estudio, fue conformado por 120 unidades experimentales que utilizaron el análisis de varianza (ANOVA) y el test de Tukey para evaluar las diferencias estadísticas y significancia estadística. Como resultado: los halos de inhibición de las concentraciones al 100% de los aceites esenciales fueron: canela 33.3 mm, limón 36.67 mm contra Acinetobacter baumannii ATCC 19606 y canela 32 mm, limón 32.67 mm contra Klebsiella pneumoniae ATCC 700603. Concluyendo que el efecto antibacteriano de los aceites esenciales de Cinnamomum verum (canela) y Citrus limon (limón), es potencialmente significativos, por manifestar efecto inhibitorio semejante a los controles positivos (Meropenem y Tazobactam) en concentraciones de 100%
398 and 19606 ACVs colocalize with the late marker LAMP1.
No full text(A) Single channel images of the inset micrograph shown in panel 2B. Bars: 10 μm. (B) J774A.1 macrophages were infected with strains GFP-398 or GFP-19606 and fixed 4 hpi. The samples were stained to observe cell nuclei (blue), GFP-A. baumannii (green) and LAMP1 (red). Representative confocal images of the infections are shown. White arrows indicate ACVs that colocalize with the marker LAMP1. Bars: 20 μm. Insets (40 μm) are a higher magnification of region indicated in the corresponding image with a white box. (C) Quantification of LAMP1+ 398 or 19606 ACVs. At least 200 infected cells were analyzed. The results are expressed as means ± SEM of three independent experiments. (TIF)</p
Predicted Lipid A biosynethic pathway in <i>A</i>. <i>baumannii</i> ATCC 19606.
No full textPredicted Lipid A biosynethic pathway in A. baumannii ATCC 19606.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Schematic of predicted lipid A and FASII biosynthetic pathway in <i>A</i>. <i>baumannii</i> ATCC 19606.
No full textSchematic of predicted lipid A and FASII biosynthetic pathway in A. baumannii ATCC 19606.</p
19606 decreases the luminal pH of the ACV during infection.
No full text(A) J774A.1 cells infected with mCherry-19606 (red) were incubated with LysoSensor (green) 15 minutes before the indicated time points. Samples were analyzed by in vivo confocal microscopy. Bars: 20 μm. (B) Analysis of the Mean Fluorescence Intensity (MFI) signal of LysoSensor per ACV at different times pi. Dotted lines show the median. (C) Percentage of ACVs that colocalize with LysoSensor at 2, 4, and 6 hpi. Statistical analyses were performed using one way ANOVA-test, **** (TIF)</p
Genomic regions of all ESGC along the genome of ATCC 19606.
No full textGraphical representations of the genomic regions for each ESGC with RD > 0 (see Methods) along the genome of ATCC 19606 with abbreviated abundance profiles and functional annotations. (PDF)</p
Pfam domains annotated in type IVa pilus proteins of <i>A</i>. <i>baumannii</i> ATCC 19606.
No full textPfam domains annotated in type IVa pilus proteins of A. baumannii ATCC 19606.</p
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