1,721,760 research outputs found
CIL:13883
No full textConstitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (this image) and anaphase cells (CIL#13886). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-CNM67–TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose. Image shows localization of Cnm67-Tem1 (eGFP, green) in metaphase after cells were transferred to medium with 2% glucose. Tem1 normally localizes preferentially to the SPB that enters the daughter cell during anaphase (CIL# 13882, 13885). Cnm67 is an integral component of the outer plaque of the SPB and the Cnm67-Tem1 fusion is found to be symmetric from SPB duplication until the end of mitosis. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1A, middle panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887
Antibiotic MICs <i>K</i>. <i>pneumoniae</i> ATCC 13883 and porin mutants.
No full textAntibiotic MICs K. pneumoniae ATCC 13883 and porin mutants.</p
SDS-PAGE of outer membrane proteins. M: Marker; 13883: ATCC13883; B1: strain with OmpK36 deficiency.
No full text<p>SDS-PAGE of outer membrane proteins. M: Marker; 13883: ATCC13883; B1: strain with OmpK36 deficiency.</p
<i>In vivo</i> competition experiments in <i>K</i>. <i>pneumoniae</i> ATCC 13883 and porin mutants.
No full textThe relative fitness of porin mutants in comparison with parental strain (ATCC 13883) or between porin mutants was determined by competition experiments in co-cultures. The values for each mouse are represented individually. Violet diamond, ATCC 13883 wild type strains. Orange square, ΔOmpK35 mutant. Red square, ΔOmpK36 mutant. Green square, OmpK36GD mutant. Blue circle, ΔOmpK35ΔOmpK36 mutant. Pink circle, ΔOmpK35OmpK36GD mutant.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Antibiotic Potential Of Sugarcane Endophytic Fungal In Klebsiella Pneumoniae ATCC 13883 And ESBL
No full textPurpose: This study aims to determine the antibiotic potential of sugarcane endophytic fungal isolates (Saccharum officinarum)
against Klebsiella pneumoniae ATCC 13883 and ESBL.
Methodology: This study was carried out at the Microbiology Laboratory of Muhammadiyah University of Surakarta's Faculty of
Pharmacy as a laboratory experiment using a posttest only control group design. Cultured on Mc Conkey medium and incubated at
37°C for 24-48 hours, the samples used were Klebsiella pneumoniae ATCC 13883 and ESBL. Finding out which sugarcane
endophytic fungus isolates are antibiotic active by measuring the inhibition zone diameter in millimeters (mm). Five groups were
formed from the total number of samples. One group served as a control, while the other two groups received antibiotics in the form of
Ciprofloxacin or dimethylsulfoxide (DMSO). The third group tested the effects of sugarcane endophytic fungal isolates (Saccharum
officinarum) at 100%, 50%, and 25% crude antibiotic concentrations. Following a Mann-Whitney post hoc test, the data was subjected
to a number of statistical tests, including a normality test with the Shapiro-Wilk, a homogeneity test with the Levene, and a Kruskall�Wallis test.
Results: The results of this study found that there is an antibiotic effect of sugarcane endophytic fungus isolates (Saccharum
officinarum) against Klebsiella pneumoniae ATCC 13883 and ESBL.
Applications/Originality/Value: Addressing the global health challenge of antibiotic resistance by exploring natural antibacterials,
namely sugarcane endophytic fungal isolates, which are considered more effective in killing bacteri
Lung infection experiments in <i>K</i>. <i>pneumoniae</i> ATCC 13883 and 10.85 as well as their isogenic porin mutants ΔOpmK35/OmpK36GD.
No full textSurvival curves after intranasal infection are represented in panels A1 (for ATCC 13883) and A2 (for 10.85). Organ burden after 24, 48 or 72h is represented in panels B1 to D1 for ATCC 13883 and B2 to D2 for 10.85. Violet diamond, ATCC 13883 or 10.85 wild type strains. Pink circle, ΔOmpK35OmpK36GD mutant. For survival studies the results were analysed using Long-rank (Mantel-Cox) test and Gehan-Breslow-Wilcoxon test. To compare bacterial load in organs during lung infection, results were compared using Mann-Whitney unpaired t tests. The analyses were performed using Prism7 (GraphPad Software). The differences between the wild type and the porin mutants were not statistically significant (P < 0.05) in any case.</p
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
